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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
No data
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Direct plate incorporation method, except the second test with S9 mix (with activation), according to the preincubation method (1 hour, 37°c))
Deviations:
not specified
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
419-690-3
EC Name:
-
Cas Number:
88020-55-7
Molecular formula:
[(C12H25)3NH]4Mo8O26
IUPAC Name:
tridodecylamine hydrate molybdenum

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Additional strain / cell type characteristics:
other: 5 strains of bacteria S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA102
Metabolic activation:
with and without
Metabolic activation system:
S9 mix, prepared from a liver microsomiale fraction (S9) of rats induced by with AROCHLOR 1254.
Test concentrations with justification for top dose:
* without S9 mix : 312.5, 625, 1250, 2500, 5000µg/plate for both tests
* with S9 mix :
TA 1537, TA 98 and TA 102 : 312.5, 625, 1250, 2500, 5000µg/plate for both tests
TA 1535 and TA 100:
62.5, 125, 250, 500, 1000µg/plate for the first test
156.25, 312.5, 625, 1250, 2500µg/plate for the second test
Vehicle / solvent:
Solvent: Acetone
Controlsopen allclose all
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
sodium azide
Remarks:
Without S9 mix
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
9-aminoacridine
Remarks:
Without S9 mix
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
2-nitrofluorene
Remarks:
Without S9 mix
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
mitomycin C
Remarks:
Without S9 mix
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: 2µg/plate of 2-anthramine : TA 1535, TA 1537, TA98 and TA 100 - 30µg/plate of Danthron : TA 102
Remarks:
With S9 mix
Details on test system and experimental conditions:
Concentration of the test substance resulting in precipitation: 1000 µg/plate

Results and discussion

Any other information on results incl. tables

The test substance did not induce any significant increase in the number of revertants, with or without S9 mix, in any of the 5 strains.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

The NORCAT M dit not show any mutagenic activity in this baterial reverse mutation assay on S.Typhimurium.