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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Clarke (2004): The test material attained 28 % degradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of the test guidelines.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The ready biodegradability of the test material was assessed in a CO2 Evolution Test. The test material, at a concentration of 10 mg C/L, was exposed to activated sewage sludge micro-organisms with culture medium in sealed culture vessels in the dark at 21 °C for 28 days. The degradation of the test material was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the standard material, sodium benzoate, together with a toxicity control were used for validation purposes.

The test was conducted in accordance with the OECD 301B, EU Method C.4-C and EPA OPTS 835.3100 guidelines.

The test material attained 28 % degradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of the study guidelines.