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EC number: 201-964-7 | CAS number: 90-05-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Despite the authors stated, this study was not in accordance with the OECD guideline since filter paper was used instead of soil. No information was available about the test substance.
Data source
Reference
- Reference Type:
- publication
- Title:
- Mechanism-based quantitative structure-phytotoxicity relationships comparative inhibition of substituted phenols on root elongation of Cucumis sativus
- Author:
- Wang X., Wang Y., Chunsheng L. and Wang S.H.
- Year:
- 2 002
- Bibliographic source:
- Arch. Environ. Contam. Toxicol., 42 : 29-35
Materials and methods
- Principles of method if other than guideline:
- Root elongation inhibition method in accordance with the OECD guideline.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Guaiacol
- EC Number:
- 201-964-7
- EC Name:
- Guaiacol
- Cas Number:
- 90-05-1
- Molecular formula:
- C7H8O2
- IUPAC Name:
- Phenol
- Details on test material:
- The test substance of sufficient purity was provided by the Department of Chemistry, Nanjing University.
Constituent 1
Test organisms
- Species:
- Cucumis sativus
- Details on test organisms:
- The seeds of Cucumis sativus were employed for test and were purchased commercially. The seeds were sterilized with 0.1% NaClO solution for 20 min., soaked for 10 min. and washed three times with deionised water prior to use.
Study design
- Test type:
- seed germination/root elongation toxicity test
- Study type:
- laboratory study
- Substrate type:
- filter paper
- Total exposure duration:
- 2 d
Test conditions
- Details on test conditions:
- The stock solution of test substance has been prepared by adding the accurately weighed chemical to dilution water (deionised water) and stirring for a minimum of 20 hours.
Range-finding tests were performed to determine the range of the test solutions and the dilution factors.
The test were conducted using 100x15 mm disposable Petri dishes and Whatman No.1 filter paper.
Fifteen pre-treated, undamaged, and plump seeds of almost identical size were placed evenly on the filter paper in each dish that was filled with 5 mL test solution or deionised water in the control.
The dishes were sealed with Parafilm during the period of incubation to avoid the test substance evaporation.
Six concentrations in geometric series were set, ranging from no effect to 100% inhibition concentration, and four replicates were set for each concentration.
The pH value of each solution was measured by using PHS23-pH meter.
Solutions were renewed every 12 hours to achieve semi-static exposure.
Deionised water without test compounds, served as control.
After 48 hours of incubation in the dark at 25 +/- 1°C, the root elongation of each seed was measured to 1 mm and RC50 (in mg/L) was calculated by the log-linear regression analysis in STATISTICA software.
The Quantitative Structure-Activity Relationship was carried out in parallel to estimate the Log 1/RC50 with physicochemical descriptors of guaiacol (solubility, Log Kow, pH and pKa).
Results and discussion
Effect concentrations
- Species:
- Cucumis sativus
- Duration:
- 2 d
- Dose descriptor:
- EC50
- Effect conc.:
- 201.33 other: mg/l
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Root elongation inhibition.
- Details on results:
- - Element value : The EC50 was measured at 25°C and pH 6.21.
- Biological observation in the control test : The germination rate root elongation in the control test was investigated to observe the reproducibility and stability of the germination rate and root elongation of Cucumis sativus.
The average germination rate was 81.26 +/- 4.45% and the average root length was 21.70 +/- 2.3 mm for the control test on 2 days.
- Biological observation with the test substance : No information was available.
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