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EC number: 201-964-7 | CAS number: 90-05-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Particle size distribution (Granulometry)
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
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- Long-term toxicity to aquatic invertebrates
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- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The purity of the test substance was known. The authors cited the Ames et al. (1975) methods paper with a detailed protocol following the cited procedure. The results were described in details.
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity testing in the Salmonella typhimurium assay of phenolic compounds and phenolic fractions obtained from smokehouse smoke condensates
- Author:
- Pool B.L., Lin P.Z.
- Year:
- 1 982
- Bibliographic source:
- Food and Chemical Toxicology, 20, 383-391.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Guaiacol
- EC Number:
- 201-964-7
- EC Name:
- Guaiacol
- Cas Number:
- 90-05-1
- Molecular formula:
- C7H8O2
- IUPAC Name:
- Phenol
- Details on test material:
- Guaiacol was purchased from Kluka, Buchs and was 98% pure or of analytical grade.
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 pre-treated Sprague-Dawley rats.
- Test concentrations with justification for top dose:
- 0, 0.5, 5, 50, 500, 5000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: see details below
- Details on test system and experimental conditions:
- POSITIVE CONTROL(S) SUBSTANCE:
2-Nitrofluorene: 3 µg/plate for TA 98 and TA 1538 without metabolic activation
Sodium azide: 2 µg/plate for TA 1535 and TA 100 without metabolic activation
9-Aminoacridine: 60 µg/plate for TA 1537 without metabolic activation
2-Aminoanthracene: 5 µg/plate for all strains with metabolic activation
DETAILS ON TEST SYSTEM AND CONDITIONS:
S. Typhimurium strains TA 1535, TA 1537, TA 1538, TA 98, and TA 100 were kindly supplied by Prof. B.N. Ames (University of Berkeley, Calif., USA).
The test for their reversion to histidine prototrophy was performed using the plate incorporation assay as previously described (Ames, McCann & Yamasaki, 1975). Each compound was dissolved in DMSO and tested in 5 concentrations in the presence of buffer ph 7.4 or S9 mix.
METHOD OF APPLICATION: in agar (plate incorporation)
Exposure duration: 48 hours - Evaluation criteria:
- The number of histidine revertants were scored and compared to the number of spontaneously arising revertants.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- the concentration of 5000 µg/plate resulted in toxicity which was apparent as a thinning of the background lawn.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table of results:
Concentration [µg/plate] |
No. of revertants per plate |
No. of revertants per plate |
Cytotoxicity |
|
(yes/no) |
||||
|
— MA |
+ MA |
|
|
TA 100 |
0 |
67 |
76 |
no |
0,5 |
66 |
94 |
no |
|
5 |
71 |
93 |
no |
|
50 |
65 |
81 |
no |
|
500 |
67 |
95 |
no |
|
5000 |
57 |
64 |
no |
|
Sodium azide / 2-Aminoanthracene |
502 |
723 |
no |
|
TA 1535 |
0 |
24 |
9 |
no |
0,5 |
21 |
16 |
no |
|
5 |
26 |
13 |
no |
|
50 |
22 |
19 |
no |
|
500 |
15 |
16 |
no |
|
5000 |
25 |
9 |
no |
|
Sodium azide / 2-Aminoanthracene |
526 |
306 |
no |
|
TA 1537 |
0 |
5 |
14 |
no |
0,5 |
7 |
12 |
no |
|
5 |
7 |
16 |
no |
|
50 |
4 |
13 |
no |
|
500 |
4 |
9 |
no |
|
5000 |
0 |
13 |
yes (-S9) |
|
9-Aminoacridine / 2-Aminoanthracene |
810 |
269 |
no |
|
TA 98 |
0 |
19 |
19 |
no |
0,5 |
20 |
34 |
no |
|
5 |
18 |
36 |
no |
|
50 |
23 |
14 |
no |
|
500 |
22 |
16 |
no |
|
5000 |
16 |
5 |
yes (+S9) |
|
2-Nitrofluorene/ 2-Aminoanthracene |
275 |
1093 |
no |
|
TA 1538 |
0 |
5 |
10 |
no |
0,5 |
13 |
12 |
no |
|
5 |
9 |
11 |
no |
|
50 |
10 |
11 |
no |
|
500 |
14 |
9 |
no |
|
5000 |
13 |
10 |
no |
|
2-Nitrofluorene/ 2-Aminoanthracene |
284 |
1027 |
no |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
- INTERPRETATION OF RESULTS:
Regarding results and applying actual criteria evaluation, the test item presented no mutagenic activity with and without S9 mix in
Salmonella Typhimurium in all strains.
Reproductibility between the experiment and positive control results in accordance with positive criteria, validated the study. - Executive summary:
In a reverse gene mutation assay in bacteria (Pool et al., 1982), strains TA 98, TA 100, TA 1535, TA 1537 and TA 1538 of S. typhimurium were exposed to guaiacol at concentrations of 0 to 5000 µg/plate in the presence and absence of mammalian metabolic activation.
Guaiacol was tested up to cytotoxic concentrations and was negative.
Cytotoxicity was observed at 5000 µg/plate.
The positive controls induced the appropriate responses in the corresponding strains. There was no evidence of induced mutant colonies over background.
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