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Diss Factsheets
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EC number: 403-830-5 | CAS number: 89331-94-2 B 290; BK 400; CK 34; DIBUTYL-N-102; DX-20; FAT NR. 40391/A; FLUORAN BLACK BD 869; FLUORAN SCHWARZ BD 869; NOIR FLUORANE BD 869; ODB-2; PSD-290; SENOR-2; TG-31; TH-108; WINCON-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988-10-31 to 1988-11-02
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984, Part I
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Remarks:
- 1 % Tween 80
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Preliminary Solution in 1 % Tween 80-tetrahydrofuran. 100 mg test substance was dissolved in auxiliary solvent and the volume made up to 10 mL. 100 µL of this stock solution was added to 1 litre diluent to give the required test concentration.
- Differential loading: 20 mL test solution/organism
- Controls: untreated control, solvent control
- Chemical name of vehicle: organic solvent - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water Flea
- Strain: Daphnia magma STRAUS
- Source: laboratory culture by IRCHA, France
- Feeding during test: Yes
- Food type: fry fish food (Liquifry) and a suspension of mixed algae (predominantly Chlorella spp.)
- Frequency: daily
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Gravid adults were isolated 24 h prior to test initiation. Young daphnids produced overnight were used for testing. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 350 mg/L (as CaCO3)
- Test temperature:
- 20 ± 2 °C
- pH:
- 8.0
- Dissolved oxygen:
- 8.6 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Jars
- Type: open
- Material, fill volume: glass, 200 mL
- Aeration: None
- Renewal rate of test solution: None
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated and aged laboratory tap water.
OTHER TEST CONDITIONS
- Photoperiod: light/dark 16/8 h
EFFECT PARAMETERS MEASURED: Mobility
VEHICLE CONTROL PERFORMED: yes
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 1 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: None obeserved
- Mortality of control: None - Validity criteria fulfilled:
- not specified
- Conclusions:
- In an acute toxicity test with daphnia magna according to OECD guideline 202 an EC50 of above 1.0 mg/L was observed.
- Executive summary:
Acute aquatic toxicity in daphnia was determined according to OECD guideline 202, Part I (Huntingdon, 1989). Daphnia magna were exposed to the test substance for 48 hours. Due to the limited solubility of the test substance in water, 1 mg/L was the highest tested concentration that could be prepared. After 48 treatment, no mortality or impaired mobility was observed in control or treatment group. A 48-hour EC50 value of above 1 mg/L was determined.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- as cited in 84/449/EEC
- GLP compliance:
- yes
- Vehicle:
- yes
- Details on test solutions:
- Concentration of vehicle in test medium: Dimethylformamide containing 1% Tween 80 was used as an auxiliary solvent at a level of 0.1 g/L- Test organisms (species):
- other: Daphnia similis
- Hardness:
- 80 mg CaCO3/L
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 49 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In an acute daphnia test an EC50 of 49 mg/L was observed after 24 h.
- Executive summary:
Short term aquatic toxicity was determined according to EU method C.2 (NIPPON SODA, ECHA RSS, 2017). Daphnia similis was exposed to the test substance for 24 hours. An EC50 of 49 mg/L was determined.
Referenceopen allclose all
Description of key information
In the acute toxicity key study with daphnia magna an EC50 above 1.0 mg/L of the test substance was determined.
Key value for chemical safety assessment
Additional information
Key study
Acute aquatic toxicity in daphnia was determined according to OECD guideline 202, Part I (Huntingdon, 1989). Daphnia magna were exposed to the test substance for 48 hours. Due to the limited solubility of the test substance in water, 1 mg/L was the highest tested concentration that could be prepared. after 48 -hour treatment, no mortality or impaired mobility was observed in control or treatment group. A 48-hour EC50 value of above 1.00 mg/L was determined.
In accordance with Article 25(3) of the REACH Regulation the robust study summaries submitted 12 years previously, are additionally used for the purpose of the registration.
Supporting study
Short term aquatic toxicity was determined according to EU method C.2 (NIPPON SODA, ECHA RSS, 2017). Daphnia similis was exposed to the test substance for 24 hours. An EC50 of 49 mg/L was determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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