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EC number: 202-025-4 | CAS number: 90-93-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 March 2018 - 30 March 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 442D (In Vitro Skin Sensitisation: ARE-Nrf2 Luciferase Test Method)
- Version / remarks:
- adopted February, 2015
- Deviations:
- no
- GLP compliance:
- yes
- Type of study:
- activation of keratinocytes
Test material
- Reference substance name:
- 4,4'-bis(diethylamino)benzophenone
- EC Number:
- 202-025-4
- EC Name:
- 4,4'-bis(diethylamino)benzophenone
- Cas Number:
- 90-93-7
- Molecular formula:
- C21H28N2O
- IUPAC Name:
- 4-[4-(diethylamino)benzoyl]-N,N-diethylaniline
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch No.of test material: 171116
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature protected from light
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dissolved in DMSO
Results and discussion
- Positive control results:
- EC1.5 of the positive control was between 5 and 125 µM (61 µM and 98 µM in experiment 1 and 2, respectively). A dose response was observed and the induction at 250 µM was higher than 2-fold (2.75-fold and 2.72-fold in experiment 1 and 2, respectively).
In vitro / in chemico
Resultsopen allclose all
- Key result
- Run / experiment:
- other: Experiment 1
- Parameter:
- other: Imax
- Value:
- 5.98
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of skin sensitisation
- Key result
- Run / experiment:
- other: Experiment 2
- Parameter:
- other: Imax
- Value:
- 5.05
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of skin sensitisation
- Other effects / acceptance of results:
- The luciferase activity induction obtained with the positive control, Ethylene dimethacrylate glycol, was above the threshold of 1.5-fold in at least one concentration.
The EC1.5 of the positive control was between 5 and 125 µM (61 µM and 98 µM in experiment 1 and 2, respectively). A dose response was observed and the induction at 250 µM was higher than 2-fold (2.75-fold and 2.72-fold in experiment 1 and 2, respectively).
The average coefficient of variation of the luminescence reading for the vehicle (negative) control DMSO was below 20% (8.8% and 14% in experiment 1 and 2, respectively).
Any other information on results incl. tables
Overview EC1.5, Imax, IC30and IC50Values
EC1.5(µM) | Imax | IC30(µM) | IC50(µM) | |
Test item Experiment 1 | <0.98 | 5.98 | 3.6 | 5.4 |
Test item Experiment 2 | <0.49 | 5.05 | 4.1 | 6.8 |
Pos Control Experiment 1 | 61 | 2.75 | N/A | N/A |
Pos Control Experiment 2 | 98 | 2.72 | N/A | N/A |
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (skin sensitising) based on GHS criteria
- Conclusions:
- In conclusion, the substance is classified as positive (activation of the antioxidant/electrophile responsive element (ARE)-dependent pathway in keratinocytes) under the experimental conditions described.
- Executive summary:
The ability of the substance to activate the antioxidant/electrophile responsive element (ARE)-dependent pathway was evaluated in the in vitro KeratinoSens assay. Two independent experiments were performed. 4,4’-Bis(diethylamino)benzophenone showed toxicity (IC30values of 3.6µM and 4.1µM and IC50values of 5.4µM and 6.8 µM in experiment 1 and 2, respectively). A biologically relevant, dose-related induction of the luciferase activity (EC1.5values of < 0.98µM and < 0.49µM in experiment 1 and 2, respectively) was measured in both experiments. The maximum luciferase activity induction (Imax) was 5.98-fold and 5.05-fold in experiment 1 and 2 respectively. The substance is positive in the KeratinoSensTMassay since positive results (>1.5-fold induction) were observed at test concentrations < 1000µM with a cell viability of >70% compared to the vehicle control.
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