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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Based on the available data, it can be concluded that the available data on genotoxicity from the source substances MDEA-Esterquat C16-18 and C18 unsatd., MDIPA-Esterquat C16-18 and C18 unsatd., and MDIPA-Esterquat C18 unsatd. can be applied to the target substance MDEA-Esterquat C18 unsatd. There is no evidence of mutagenic potential.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that source and target substances have similar toxicological and ecotoxicological properties because they share structural similarities with common functional groups: quaternary amines, esters, and fatty acid chains varying in their length and degree of (un)saturation. Moreover, the fatty acid chains are chemically simple structures which have no structural alerts for toxicity, and which are closely related to substances of known low toxicity (i.e. stearic acid, oleic acid, linoleic acid, linolenic acid). Furthermore, the substances can be expected to have comparable breakdown products (MDEA or MDIPA and long chain fatty acids).
This read-across hypothesis corresponds to scenario 2 - different compounds have qualitatively and quantitatively the same type of effects - of the read-across assessment framework i.e. properties of the target substance MDEA-Esterquat C18 unsatd. are predicted to be similar to those of the source substances MDIPA Esterquat C18 unsatd., MDEA-Esterquat C16-18 and C18 unsatd. and MDIPA-Esterquat C16-18 and C18 unsatd.
Therefore, read-across from the available physicochemistry, toxicity and ecotoxicity studies with the source substances MDIPA Esterquat C18 unsatd., MDEA-Esterquat C16-18 and C18 unsatd. and MDIPA-Esterquat C16-18 and C18 unsatd. are considered as an appropriate adaptation to the standard information requirements of the REACH Regulation for the target substance MDEA-Esterquat C18 unsatd., in accordance with the provisions of Annex XI, 1.5 of the REACH Regulation.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
please refer to read-across justification attached to Iuclid section 13
3. ANALOGUE APPROACH JUSTIFICATION
please refer to read-across justification attached to Iuclid section 13
4. DATA MATRIX
please refer to read-across justification attached to Iuclid section 13 - Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across: supporting information
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not applicable
- Positive controls validity:
- not applicable
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not applicable
- Positive controls validity:
- not applicable
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not applicable
- Positive controls validity:
- not applicable
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not applicable
- Positive controls validity:
- not applicable
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not applicable
- Positive controls validity:
- not applicable
- Conclusions:
- negative
Based on read-across from the source substances MDIPA Esterquat C18 unsatd., MDIPA Esterquat C16-18 and C18 unsatd. and MDEA-Esterquat C16-18 and C18 unsatd., the target substance MDEA-Esterquat C18 unsatd. is not mutagenic in the bacterial reverse mutation assay.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
No experimental data are available for the target substance MDEA-Esterquat C18 unsatd. However, bacterial reverse mutation assays are available for the structurally related source substances MDEA-Esterquat C16-18 and C18 unsatd., MDIPA-Esterquat C16-18 and C18 unsatd., and MDIPA-Esterquat C18 unsatd.
A justification for read-across is attached to Iuclid section 13.
The mutagenic potential of MDEA-Esterquat C16-18 and C18 unsatd. was evaluated in the Bacterial Mutation Test (GLP Ames). The test substance was tested in the bacterial reverse mutation assay using Salmonella typhimurium tester strains TA98, TA100, TA1535, and TA1537 and Escherichia coli tester strain WP2 uvrA (pKM101) in the presence and absence of Aroclor-induced rat liver S9. The assay was performed in two phases, using the plate incorporation method. The first phase, the initial mutagenicity assay, was used to establish the dose-range for the confirmatory mutagenicity assay and to provide a preliminary mutagenicity evaluation. The second phase, the confirmatory mutagenicity assay, was used to evaluate and confirm the mutagenic potential of the test substance.
In the initial mutagenicity assay, the maximum dose tested was 5000 ug/plate; this dose was achieved using a concentration of 50 mg/ml and a 100 ul plating aliquot. In the initial mutagenicity assay, the dose levels tested were 1.5, 5.0, 15, 50, 150, 500, 1500, and 5000 ug/plate. No positive mutagenic responses were observed. Precipitate was observed beginning at 1500 or at 5000 ug/plate. Toxicity was observed in the absence of S9 activation beginning at 1500 or at 5000 ug/plate with tester strains TA98, TA100 and TA1537.
In the confirmatory mutagenicity assay, no positive mutagenic responses were observed with any of the tester strains in the presence or absence of S9 activation. The dose levels tested were 15, 50, 150, 500, 1500 and 5000 ug/plate. Precipitate was observed beginning at 1500 or at 5000 ug/plate. Toxicity was observed beginning at 1500 or at 5000 ug/plate with tester strains TA98, TA100, TA1537 and WP2 uvrA (pKM101) in the absence of S9 and with tester stains TA100 and TA1535 in the presence of S9 activation.
Under the conditions of this study, the test substance was concluded to be negative with Salmonella typhimurium tester strains TA98, TA100, TA1535 and TA1537 and Escherichia coli tester strain WP2 uvrA (pKM101) in the presence and absence of Aroclor-induced rat liver S9.
In a reverse gene mutation assay in bacteria according to OECD guideline 471, strains TA 1535, TA 1537, TA 98, TA 100 of S. typhimurium and E. coli WP2 were exposed to MDIPA-Esterquat C16-18 and C18 unsatd. (100% a.i.) in ethanol at concentrations of 0, 3, 10, 33, 100, 333, 1000, 3330 and 5000 μg/plate in the first experiment and 0, 10, 33, 100, 333 and 1000 µg/plate (Salmonella strains) and 0, 100, 333, 1000, 3330 and 5000 µg/plate (E. coli strain) in the second experiment in the presence and absence of mammalian metabolic activation (S9 mix).
MDIPA-Esterquat C16-18 and C18 unsatd. was tested up to cytotoxic concentrations. There was no evidence of induced mutant colonies over background.
In a reverse gene mutation assay in bacteria according to OECD guideline 471, strains TA98, TA100, TA1535, and TA1537 of Salmonella typhimurium and Escherichia coli WP2 uvrA were exposed to MDIPA Esterquat C18 unsatd. (100% a.i.) in acetone at concentrations of 0 (control), 62, 185, 556, 1667 and 5000 µg/plate in the first experiment and 0 (control), 22, 67, 200, 600 and 1800 µg/plate in the second experiment in the presence and absence of mammalian metabolic activation (rat liver S9 mix). The assay was performed using the plate incorporation method.
MDIPA Esterquat C18 unsatd. was tested up to cytotoxic concentrations. Cytotoxic effects were noted starting at 1667 µg/plate in strains TA 1535 without metabolic activation, TA 1537, TA 98 and TA 100 with and without metabolic activation and at 5000 µg/plate in TA 1535 with metabolic activation; no cytotoxic effects were seen in E. coli WP 2 uvrA.
Precipitation was observed when the test substance was pipetted into the top agar and later on the agar plates beginning at 556 µg/plate with and without metabolic activation.
There was no evidence of induced mutant colonies over background. Under the conditions of the study, the test substance was negative for mutagenic potential.
Conclusion
Based on the available data, it can be concluded that the available data on genotoxicity from the source substances MDEA-Esterquat C16-18 and C18 unsatd., MDIPA-Esterquat C16-18 and C18 unsatd., and MDIPA-Esterquat C18 unsatd. can be applied to the target substance MDEA-Esterquat C18 unsatd. There is no evidence of mutagenic potential.
Justification for classification or non-classification
Based on relevant, reliable and adequate data MDIPA Esterquat C18 unsatd. does not need to be classified and labelled according to the CLP Regulation (EC) No 1272/2008 with respect to mutagenicity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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