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EC number: 473-390-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 April 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conducted under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 473-390-7
- EC Name:
- -
- Cas Number:
- 1093615-61-2
- Molecular formula:
- C7F15NO
- IUPAC Name:
- 2,2,3,3,5,5,6,6-octafluoro-4-(1,1,1,2,3,3,3-heptafluoropropan-2-yl)morpholine; 2,2,3,3,5,5,6,6-octafluoro-4-(1,1,2,2,3,3,3-heptafluoropropyl)morpholine
- Reference substance name:
- MTDID 7145
- IUPAC Name:
- MTDID 7145
- Details on test material:
- - Name of test material (as cited in study report): MTDID 7145
- Substance type: Multiconstituent substance
- Physical state: Clear, colourless liquid
- Analytical purity: 94.5%
- Lot/batch no.: 142072:43
- Expiration date of the lot/batch: 30 Dec 2007
- Stability under test conditions: Stable
- Storage condition of test material: At room temperature in the dark
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Because of low solubility, FC-770 was added to reverse osmosis-purified water at a loading rate of 2000 mg/L, with 24 hours' stirring in closed 1-L test bottles. Note that during weighing some of the test substance was lost due to evaporation. Therefore, the initially weighed amount of test substance was slightly higher, i.e. approximately 520 mg. For the test, synthetic sewage feed (16 mL) and prepared, activated sludge (200 mL) were added to bring the loading rate to 1000 mg/L FC-770.
-Control: blanks, 3,5-Dichlorophenol as reference substance
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: Sludge was collected from municipal sewage treatment plant: 'Waterschap de Maaskant', 's-Hertogenbosch, the Netherlands.The sludge was coarsely sieved, washed and diluted with ISO medium. A small amount of the sludge was weighed and dried at ca. 105°C to determine the amount of suspended solids (3.9 g/L of sludge, as used for the test). The sludge was collected one day before use. Before use the pH was checked (measured value: 6.1).
ISO medium (in reverse osmosis [RO]-purified tap water)
211.5 mg/L CaCl2∙2(H2O)
88.7 mg/L MgSO4∙7(H2O)
46.7 mg/L NaHCO3
4.2 mg/L KCl
ISO medium was used only for sludge preparation, not during the test.
- Method of cultivation: Because the sludge was used one day after collection, 50 mL of synthetic sewage feed (16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCI, 0.4 g CaCI2∙2(H2O), 0.2 g MgSO4∙7(H20), 2.8 g K2HPO4 dissolved in 1 L RO water and filtered. The pH was 7.2) was added to each litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not reported
- Test temperature:
- 18.2 - 20.4 °C
- pH:
- 6.9 - 7.5
- Dissolved oxygen:
- Continuous aeration throughout exposure period. Measured dissolved oxygen [DO] values at start of observation period were 7.4-9.1 mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentration 1000 mg/L during exposure period. No analytical monitoring
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Exposure (3 hours) in aerated 1-L bottles. Observation/DO measurement in sealed, 300 mL oxygen bottles.
- Type (delete if not applicable): Open during exposure. Closed during observation.
- Material, size, headspace, fill volume: 250 mL test solution, 16 mL synthetic sewage, and 200 mL sludge suspension plus sufficient RO water to bring total volume to 500 mL. Well mixed sample of the contents was poured into a 300 mL oxygen bottle, and the flask was sealed with an oxygen electrode connected to a recorder, forcing the air out of the vessel (zero headspace).
- Aeration: Continuous aeration with pipette, and stirring
- No. of vessels per concentration (replicates): Two for FC-770, one for reference substance 3,5-dichlorophenol
- No. of vessels per control (replicates):Two
- Biomass loading rate: 1.56 g/L Mixed Liquor Sludge Solids
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ion-exchange cartridges
- Culture medium different from test medium: Approximately 50 mL/L synthetic sewage added per day to sludge suspension during holding (maximum 4 days).
- Intervals of water quality measurement: Not specified. Water quality data not provided
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Light intensity: not specified
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Dissolved oxygen concentration
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Single concentration tested
- Justification for using less concentrations than requested by guideline: Limit test, FC-770 not soluble in water. - Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: No reduction in respiration rate was recorded at nominal 1000 mg/L FC-770.
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: yes
- Acceptability of controls: The difference between the control respiration rates was 13 % (criterion, <15 % variability). - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: The EC50 of 3,5-dichlorophenol was 5.4 mg/L (in the accepted range of 5-30 mg/L)
Any other information on results incl. tables
Table, Oxygen concentration, oxygen consumption, percentage inhibition and pH for Controls (C), 3,5-dichlorophenol (R), and FC-770 (T) |
|||||
Replicate |
Concentration of substance (mg/L) |
Initial oxygen conc. (mg/L) |
Oxygen consumption rate (mg O2/L/h) |
Respiration inhibition (%) |
pH |
C1 |
0 |
7.6 |
39 |
6.9 |
|
C2 |
0 |
6.8 |
42 |
7.0 |
|
Mean C1+C2 |
40.5 |
||||
R1 |
1.0 |
7.4 |
36 |
11 |
7.1 |
R2 |
3.2 |
7.9 |
26 |
36 |
7.5 |
R3 |
10 |
8.7 |
9 |
78 |
7.3 |
R4 |
32 |
9.1 |
3 |
93 |
7.2 |
C2 |
0 |
6.8 |
42 |
7.0 |
|
C3 |
0 |
7.9 |
37 |
7.0 |
|
Mean C2+C3 |
39.5 |
||||
T1 |
1000 |
7.0 |
42 |
-6.3 |
6.9 |
T2 |
1000 |
7.8 |
41 |
-3.8 |
6.9 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Control respiration rate variability < 15%. Reference substance 3-h EC50 5.4 mg/L (within 5-30 mg/L)
- Conclusions:
- FC-770 is not inhibitory to activated sludge respiration (3-h EC50 >1000 mg/L)
- Executive summary:
The toxicity of FC-770 to activated sludge organisms was assessed in an assay performed according to OECD TG209. The test solution was made by stirring FC-770 24 hours in a closed vessel at a loading rate of 2000 mg/L, with subsequence dilution to 1000 mg/L for the test. Undissolved FC-770 remained in the test bottles. The exposure period was for three hours with continuous aeration. No inhibition was observed upon exposure to FC-770 at the loading rate. The 3-hour EC50 to sludge is therefore >1000 mg/L. No NOEC is assigned due to volatility of FC-770.
The study was conducted in accord with GLP criteria and satisfies the guideline requirements for test method OECD209, activated sludge respiration inhibition test. It is classified as acceptable.
Results Synopsis
Test Type: Static
EC50: >1000 mg/L 95% C.I.: not applicable
NOEC: not applicable Probit Slope: not applicable
Endpoint(s) Effected: Respiration inhibition
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