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EC number: 259-393-4 | CAS number: 54914-37-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-05-19 to 2011-06-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): A fresh sample of activated sludge from the aeration basin of a municipal sewage treatment plant (Moers, Germany) was collected and aerated until usage.
- Storage condition/length: The inoculum was not fed during the aeration period. The duration of the aeration period was 1 day.
- Preparation of inoculum for exposure: After determination of the suspended solids (dry weight) a sample containing approx.
1875 mg suspended solids was filtered by means of a water jet pump through a filter paper (Whatman GmbH, black ribbon 589(1)). The sludge was washed twice with mineral medium. Afterwards, it was suspended in 250 mL mineral medium and stirred until it was homogeneous.
- Concentration of sludge: 4 mL of this suspension were used to inoculate 1 litre of mineral medium. This amount of inoculum corresponded to 30 mg/L suspended solids. - Duration of test (contact time):
- >= 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to OECD 301
- Test temperature: 22 ± 1 °C
- pH: 7.69 - 8.41
- pH adjusted: no
- Suspended solids concentration: 30 mg/L
TEST SYSTEM
- Culturing apparatus: Sensomat system (Liebherr, Aqualytic, Langen)
- Number of culture flasks/concentration: 3
- Measuring equipment: Sensomat system (Liebherr, Aqualytic, Langen)
- Details of trap for CO2 and volatile organics if used: Before test vessels were closed a CO2 trap consisting of a sodium hydroxide pellet was assembled in the vessel’s head space.
SAMPLING
- Sampling frequency: 360 times during the 28 day experimental period
- Sampling method: measurement of pressure decrease
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 vessels
- Abiotic sterile control: 2 vessels
- Toxicity control: 2 vessels
- Other: 2 vessels with reference substance, 3 vessels with test substance - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 34
- Sampling time:
- 28 d
- Details on results:
- At the end of the test period a biodegradation rate of 34 % of the ThODNH4 was measured for the test substance.
Because the biodegradation rate was < 60 % of the ThODNH4 after 28 days,
the test substance cannot be considered to be readily biodegradable under test conditions.
The test substance showed no toxicity towards microorganisms of the activated sludge, because 54 % degradation was recorded within 14 days in the toxicity control preparations containing both the test substance and the positive control substance in the same concentrations. - Results with reference substance:
- As reference substance (procedure control) sodium benzoate in a concentration of 100 mg/L was used. After 14 days the reference substance was sufficiently degraded to 88 % of the ThODNH4, thus confirming the suitability of the used activated sludge inoculum and fulfilling the validity criteria mentioned in the relevant test guideline. The reference substance was degraded to 91 % of the ThODNH4 after 28 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable, but moderate biodegradation was observed
- Conclusions:
- Because the biodegradation rate was < 60 % of the ThODNH4 after 28 days, the test substance cannot be considered to be readily biodegradable under test conditions. However, moderate biodegradation was observed (34% within 28 days)
- Executive summary:
The test substance 1,3,3-trimethyl-N-(2-methylpropylidene)-5-[(2-methylpropylidene)amino]cyclohexanemethylamine was tested in a manometric respirometry test according to the OECD Guideline for Testing of Chemicals, No. 301 F for 28 days to determine whether the test substance is readily biodegradable. At the end of the test period a biodegradation rate of 34 % of the ThODNH4was measured. Because the biodegradation rate was < 60 % of the ThODNH4 after 28 days, the test substance cannot be considered to be readily biodegradable under test conditions. As reference substance (procedure control) sodium benzoate in a concentration of 100 mg/L was used. After 14 days the reference substance was sufficiently degraded to 88 % of the ThODNH4, thus confirming the suitability of the used activated sludge inoculum and fulfilling the validity criteria mentioned in the relevant test guideline. The reference substance was degraded to 91 % of the ThODNH4 after 28 days.
Reference
Oxygen consumption and degradation rates
|
oxygen consumption [mg/L] |
degradation rates [%] |
|||||||
ThODNH4 |
|
287.3 |
166.7 |
287.3 |
454 |
|
|
|
|
time (days) |
mean value blank control |
mean value 100 mg/L abiotic control |
mean value 100 mg/L sodium benzoate |
mean value 100 mg/L test substance |
mean value 100 mg/L * toxicity control |
100 mg/L abiotic control |
100 mg/L sodium benzoate |
100 mg/L test substance |
100 mg/L * toxicity control |
0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0 |
0 |
0 |
0 |
1 |
0.1 |
-3.1 |
42.9 |
15.0 |
45.3 |
-1 |
26 |
5 |
10 |
2 |
2.1 |
-2.0 |
70.4 |
46.4 |
80.8 |
-1 |
41 |
15 |
17 |
3 |
3.7 |
-2.0 |
111.0 |
64.8 |
110.0 |
-1 |
64 |
21 |
23 |
4 |
4.9 |
-2.0 |
124.0 |
75.0 |
163.0 |
-1 |
71 |
24 |
35 |
5 |
6.1 |
-2.0 |
133.0 |
81.9 |
181.0 |
-1 |
76 |
26 |
39 |
6 |
7.1 |
-2.0 |
138.5 |
88.7 |
198.5 |
-1 |
79 |
28 |
42 |
7 |
8.0 |
-2.0 |
143.0 |
94.8 |
218.5 |
-1 |
81 |
30 |
46 |
8 |
8.9 |
-2.0 |
146.0 |
98.8 |
231.0 |
-1 |
82 |
31 |
49 |
9 |
9.7 |
-2.0 |
148.0 |
102.7 |
239.0 |
-1 |
83 |
32 |
50 |
10 |
10.7 |
-2.0 |
151.5 |
105.7 |
245.5 |
-1 |
84 |
33 |
52 |
11 |
11.3 |
-2.0 |
154.0 |
108.0 |
252.0 |
-1 |
86 |
34 |
53 |
12 |
12.3 |
-2.0 |
157.0 |
110.0 |
255.5 |
-1 |
87 |
34 |
54 |
13 |
12.8 |
-2.0 |
158.0 |
110.7 |
257.0 |
-1 |
87 |
34 |
54 |
14 |
13.6 |
-2.0 |
159.5 |
112.0 |
258.5 |
-1 |
88 |
34 |
54 |
15 |
14.0 |
-3.1 |
162.0 |
112.3 |
260.5 |
-1 |
89 |
34 |
54 |
16 |
14.7 |
-3.1 |
163.0 |
113.0 |
260.5 |
-1 |
89 |
34 |
54 |
17 |
15.2 |
-2.0 |
164.0 |
113.7 |
260.5 |
-1 |
89 |
34 |
54 |
18 |
15.6 |
-3.1 |
165.0 |
113.0 |
263.5 |
-1 |
90 |
34 |
55 |
19 |
16.4 |
-2.0 |
165.5 |
115.0 |
262.0 |
-1 |
89 |
34 |
54 |
20 |
16.5 |
-1.0 |
166.5 |
115.0 |
263.5 |
0 |
90 |
34 |
54 |
21 |
16.8 |
-2.0 |
167.5 |
115.0 |
263.5 |
-1 |
90 |
34 |
54 |
22 |
17.3 |
-2.0 |
168.5 |
115.7 |
265.0 |
-1 |
91 |
34 |
55 |
23 |
17.6 |
-1.0 |
169.0 |
116.3 |
265.0 |
0 |
91 |
34 |
54 |
24 |
17.9 |
-1.0 |
169.5 |
115.7 |
265.0 |
0 |
91 |
34 |
54 |
25 |
18.3 |
-1.0 |
170.0 |
117.0 |
265.0 |
0 |
91 |
34 |
54 |
26 |
18.3 |
0.0 |
170.5 |
116.3 |
265.0 |
0 |
91 |
34 |
54 |
27 |
18.7 |
1.1 |
171.0 |
117.7 |
265.0 |
0 |
91 |
34 |
54 |
28 |
18.8 |
2.1 |
171.0 |
117.7 |
266.5 |
1 |
91 |
34 |
55 |
* each with test substance and reference substance
Description of key information
The test substance was tested in a manometric respirometry test according to the OECD Guideline for Testing of Chemicals, No. 301 F for 28 days to determine whether the test substance is readily biodegradable. At the end of the test period a biodegradation rate of 34 % of the ThODNH4 was measured. Because the biodegradation rate was < 60 % of the ThODNH4 after 28 days, the test substance cannot be considered to be readily biodegradable under test conditions. However, the present study indicates that moderate biodegradation presumably will occur in the environment.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
Although 1,3,3-trimethyl-N-(2-methylpropylidene)-5-[(2-methylpropylidene)amino]cyclohexanemethylamine has to be considered as not readily biodegradable a significant degradation potential could be observed (34% in 28 days).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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