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EC number: 203-372-4 | CAS number: 106-20-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05. Jun 1967 - 13. Jun 1967
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable well documented report which meets basic scientific princples.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 967
- Report date:
- 1967
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- BASF-Test:
Four animals were treated for 1, 5, 15 min or 20 hours using occlusive conditions. An application site of 2.5 x 2.5 cm was covered with the liquid test substance. The animals were observed for 8 days and skin changes were recorded daily. The report describes findings after 24 hours and at the end of the observation period (8 days). For a final evaluation, the findings after 48 and 72 hours from the raw data were taken into account. - GLP compliance:
- no
Test material
- Reference substance name:
- Bis(2-ethylhexyl)amine
- EC Number:
- 203-372-4
- EC Name:
- Bis(2-ethylhexyl)amine
- Cas Number:
- 106-20-7
- Molecular formula:
- C16H35N
- IUPAC Name:
- bis(2-ethylhexyl)amine
- Details on test material:
- - Name of test material (as cited in study report): di-(2-aethylhexyl)-amin
- Analytical purity: > 99 %
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 3.1 kg (mean)
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- not specified
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- no data
- Duration of treatment / exposure:
- 1, 5, 15 min or 20 h
- Observation period:
- 8 days
- Number of animals:
- 4
- Details on study design:
- TEST SITE
- Area of exposure: 2.5 x 2.5 cm
REMOVAL OF TEST SUBSTANCE
- Washing (if done): concentrated Lutrol and 50% Lutrol
- Time after start of exposure: 1min, 5 min, 15 min and 20 h, respectively
SCORING SYSTEM: The original BASF grading was converted into the numerical grading according to the OECD Draize system.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Remarks:
- animal 1-4
- Time point:
- 24/48/72 h
- Score:
- 2.7
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 8 days
- Irritation parameter:
- edema score
- Basis:
- mean
- Remarks:
- animal 1-4
- Time point:
- 24/48/72 h
- Score:
- 1.7
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks:
- 8 days
- Irritation parameter:
- other: necrosis
- Basis:
- animal: 1-4
- Time point:
- 24/48/72 h
- Remarks on result:
- other: 15 min treatment: full thickness necrosis (1/4 animals): 7 and 8 days after treatment; necrosis - not full thickness (2/4): 6, 7 and 8 days after treatment and 7 and 8 days after treatment, respectively
- Irritant / corrosive response data:
- Exposure times of 1, 5 and 15 min caused reddening of the dorsal rabbit skin and severe edema beyond the area of exposure. One week later slight reddening (score 2), rhagades and slight necrosis (1 min and 5 min exposures: not full thickness necrosis), severe necrosis (15 min exposure: full thickness necrosis) and induration of the application site were observed. An application of undiluted bis(2-ethylhexyl)amine, 20 h exposure, led to slight reddening, severe edema and severe necrosis (full thickness necrosis). After 8 days, severe necrosis (full thickness necrosis) and induration of the application site were observed. For details see tables below.
Any other information on results incl. tables
Erythema and edema scores for each animal:
Exposition: | 1 min | Comment | ||
Animal | Reading | Erythema | Edema | |
1 | 1 min | 0 | 0 | |
2 | 1 min | 2 | 0 | |
3 | 1 min | 2 | 0 | |
4 | 1 min | 1 | 2 | |
1 | 24 h | 3 | 0 | bleedings |
2 | 24 h | 3 | 2 | |
3 | 24 h | 4 | 3 | bleedings |
4 | 24 h | 3 | 3 | |
1 | 48 h | 3 | 0 | bleedings |
2 | 48 h | / | / | not examined |
3 | 48 h | / | / | not examined |
4 | 48 h | 3 | 2 | bleedings |
1 | 72 h | 2 | 0 | |
2 | 72 h | / | / | not examined |
3 | 72 h | / | / | not examined |
4 | 72 h | 2 | 2 | bleedings |
1 | 8 days | 0 | 0 | necrosis (not full thickness), fissures |
2 | 8 days | 3 | 0 | |
3 | 8 days | 0 | 0 | necrosis (not full thickness), induration |
4 | 8 days | 2 | 0 | bluish with yellow regions, induration |
mean (animal 1 & 4)* | 24 - 48 - 72 h | 2.7 | 1.2 | |
*no mean calculation possible for animals 2 and 3, because scores on the 48 or 72 h readings are missing | ||||
Exposition: | 5 min | Comment | ||
Animal | Reading | Erythema | Edema | |
1 | 5 min | 0 | 0 | |
2 | 5 min | 2 | 0 | |
3 | 5 min | 2 | 0 | |
4 | 5 min | 1 | 2 | |
1 | 24 h | 3 | 0 | bleedings |
2 | 24 h | 3 | 2 | |
3 | 24 h | 3 | 3 | |
4 | 24 h | 3 | 3 | |
1 | 48 h | 3 | 0 | bleedings |
2 | 48 h | / | / | not examined |
3 | 48 h | / | / | not examined |
4 | 48 h | 2 | 2 | bleedings |
1 | 72 h | 2 | 0 | |
2 | 72 h | / | / | not examined |
3 | 72 h | / | / | not examined |
4 | 72 h | 2 | 0 | |
1 | 8 days | 0 | 0 | necrosis (not full thickness), fissures |
2 | 8 days | 3 | 2 | necrosis (not full thickness) |
3 | 8 days | 0 | 0 | anemic necrosis (not full thickness), induration |
4 | 8 days | 2 | 0 | bluish with yellow regions, induration |
mean (animal 1 & 4)* | 24 - 48 - 72 h | 2.5 | 0.8 | |
*no mean calculation possible for animals 2 and 3, because scores of the 48 or 72 h readings are missing | ||||
Exposition: | 15 min | Comment | ||
Animal | Reading | Erythema | Edema | |
1 | 15 min | 1 | 2 | |
2 | 15 min | 3 | 0 | |
3 | 15 min | 2 | 0 | |
4 | 15 min | 1 | 2 | |
1 | 24 h | 3 | 0 | bleedings |
2 | 24 h | 3 | 3 | |
3 | 24 h | 3 | 3 | bleedings |
4 | 24 h | 3 | 3 | |
1 | 48 h | 3 | 0 | bleedings |
2 | 48 h | / | / | not examined |
3 | 48 h | / | / | not examined |
4 | 48 h | 2 | 2 | bleedings |
1 | 72 h | 3 | 3 | bleedings |
2 | 72 h | / | / | not examined |
3 | 72 h | / | / | not examined |
4 | 72 h | 2 | 2 | bleedings |
1 | 8 days | 0 | 0 | full thickness necrosis, induration |
2 | 8 days | 0 | 0 | necrosis (not full thickness), induration |
3 | 8 days | 0 | 0 | anemic necrosis (not full thickness), induration |
4 | 8 days | 2 | 0 | bluish with yellow regions, induration |
mean (animal 1 & 4)* | 24 - 48 - 72 h | 2.7 | 1.7 | |
*no mean calculation possible for animals 2 and 3, because scores of the 48 or 72 h readings are missing | ||||
Exposition: | 20 h | Comment | ||
Animal | Reading | Erythema | Edema | |
1 | 24 h | 2 | 3 | bleedings |
2 | 24 h | 3 | 3 | induration |
3 | 24 h | 2 | 3 | anemic, necrosis (not full thickness) |
4 | 24 h | 0 | 3 | full thickness necrosis |
1 | 48 h | 2 | 3 | bleedings |
2 | 48 h | / | / | not examined |
3 | 48 h | / | / | not examined |
4 | 48 h | 0 | 3 | anemic |
1 | 72 h | 2 | 2 | anemic necrosis (not full thickness) |
2 | 72 h | / | / | not examined |
3 | 72 h | / | / | not examined |
4 | 72 h | 2 | 2 | full thickness necrosis |
1 | 8 days | 2 | 0 | anemic necrosis (not full thickness), bluish with yellow regions, induration |
2 | 8 days | 2 | 0 | full thickness necrosis |
3 | 8 days | 3 | 0 | full thickness necrosis, induration |
4 | 8 days | 0 | 0 | full thickness necrosis, induration, fissures |
mean (animal 1 & 4)* | 24 - 48 - 72 h | 1.3 | 2.7 | |
*no mean calculation possible for animals 2 and 3, because scores of the 48 or 72 h readings are missing |
Applicant's summary and conclusion
- Interpretation of results:
- Category 1B (corrosive) based on GHS criteria
- Conclusions:
- Due to the appearance of full thickness necrosis on day six after treatment (exposure period 15 min) lasting until the end of the observation period of eight days, it can be concluded that the test substance has a corrosive potential.
- Executive summary:
Four animals were treated for 1, 5, 15 min or 20 hours using occlusive conditions. An application site of 2.5 x 2.5 cm was covered with the liquid test substance. The animals were observed for 8 days and skin changes were recorded daily. The report describes findings after 24 hours and at the end of the observation period (8 days). For a final evaluation, the findings after 48 and 72 hours from the raw data were taken into account. Due to the appearance of full thickness necrosis on day six after treatment (exposure period 15 min) lasting until the end of the observation period of eight days, it can be concluded that the test substance has a corrosive potential.
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