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Diss Factsheets
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EC number: 433-480-9 | CAS number: 623-53-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: no official guideline method, GLP without certificate, good documentation, identity of substance clear and with details on its purity
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
Materials and methods
- Principles of method if other than guideline:
- guideline that is proposed by the JMHW (Japanese Ministry of Health and Welfare) and JMOL
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- -
- EC Number:
- 433-480-9
- EC Name:
- -
- Cas Number:
- 623-53-0
- Molecular formula:
- C4H8O3
- IUPAC Name:
- ethyl methyl carbonate
- Details on test material:
- Test material as stated in study report ("Name"): Ethylmethyl carbonate
Purity: 99.8 %
Molecular weight: 104
Melting point: -14.5 °C
Boiling point: 109.2 °C
Appearance at ambient temperature: colorless and transparent liquid
Stability: stable at normal temperature
Solubility: soluble in dimethylsulfoxide and acetone more than 5 wt%
Lot No.: MEC-27452
Remark of the author of this IUCLID dossier concerning the substance identity:
Every page of the copy of the study report contains an annotation referring to the data sharing agreement in place between the licensee that uses this study report and the data owner. In this data sharing agreement the identity of the chemical substance is specified with "Ethyl Methyl Carbonate - EC No. 433-480-9, CAS Nr. 623-53-0".
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 1537
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Solvent control, 10, 50, 100, 500, 1000, 5000 µg per plate
- Vehicle / solvent:
- The test substance was dissolved in dimethylsulfoxide.
Controls
- Untreated negative controls:
- yes
- Remarks:
- Dimethylsulfoxide (= solvent control)
- Positive controls:
- yes
- Positive control substance:
- furylfuramide
- Remarks:
- Migrated to IUCLID6: and: sodium azide, 9-Aminoacridine, Benzo[a]-pyrene, 2-Aminoanthracene
- Details on test system and experimental conditions:
- The following procedure was carried out with each bacterial strain:
A portion (0.1 mL) of the test substance was placed in a glass tube. Then 0.1 mL of the subculture, and 0.5 mL of S9 mix or 0.5 mL of 0.1 M sodium phosphate buffer (pH 7.4) was added to the test substance and incubated at 37 °C for 20 minutes with shaking. The suspension was then thoroughly mixed with 2.0 mL of the top agar and overlaid onto the minimum glucose agar plate. After 48 hours' incubation at 37 °C, revertant colonies were counted. Negative and positive controls were simultaneously studied. Preliminary reverse mutation test and reverse mutation test were conducted in duplicate. Toxicity of the test substance was observed with a stereoscopic microscope.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- With the test substance, increase in the number of revertant colonies was not observed in any bacterial strain used. The number of revertant colonies in positive controls increased markedly as compared with the negative control. This result indicated that this test was carried out properly. The environmental factor that might influence reliability of this test was not detected.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
other: negative mutagenic potential of the test substance
It is concluded from the foregoing results that the test substance is considered as negative for mutagenic potential under the test conditions employed (bacteria mutagenicity).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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