Benzenemethanol, 3-hydroxy-.alpha.-[[methyl(phenylmethyl)amino]methyl]-, (.alpha.S)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-[(1S)-2-[benzyl(methyl)amino]-1-hydroxyethyl]phenol

Cas Number:

286426-31-1

Molecular formula:

C17 H20 O2

IUPAC Name:

3-[(1S)-2-[benzyl(methyl)amino]-1-hydroxyethyl]phenol

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Physical state: solid
- Analytical purity: 99.7 %
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Age at study initiation : 6 - 23 hours
- Method of breeding: The daphnia were bred in the laboratorie sof IBACON under similar temperature an light conditions as in the test, and in
reconstituted water of a similar quality regarding to pH, compounds of the main ions and total hardness as the test water
used in the test. The test organisms were not the first brood progeny.
-sex : female

ACCLIMATION
- Acclimation period: 6 h
- Acclimation conditions : same as test conditions

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

250.00 mg/L

Test temperature:

galss beaker 21 ° C

pH:

7.8 - 8.8

Dissolved oxygen:

at least 8.5 m/L or higher

Nominal and measured concentrations:

The analytically determined mean test item concentartion in the analysed test media varied in the range from 93 % - 103 % of the nominal values.

Details on test conditions:

TEST SYSTEM
- Test vessel: beaker
- Material, size, headspace, fill volume: glass, 100 mL volumen with 75 mL test medium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Alkalinity: 0.8 mmol/L


OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark
- Light intensity: 500 lux


TEST CONCENTRATIONS
- Range finding study : The test concentartions were based on the results of a range-finding test.
- Test concentrations: 2.1, 4.6, 10, 21, 46 and 100 mg test item/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

75.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

63.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 7

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: The mortality of daphnia magna in the control did not exceed 10 % and no Daphnia magna were trapped at the surface.
- Influence of L-Benzyladrianol Base on the mobility of Daphnia magna : see table 1

Any other information on results incl. tables

Table 1 Influence of L-Benzyladrianol Base on the mobility of Daphnia magna

Concentration of the test item mg/L	No. of Daphnia tested	No. of immobilized Daphnia after 24 h	No. of immobilized Daphnia after 48 h	% of immobilized Daphina after 24 h	% of immobilized Daphina after 24 h
control	20	0	0	0	0
2.1	20	0	0	0	0
4.6	20	0	0	0	0
10.0	20	0	0	0	0
21.0	20	1	1	5	5
46.0	20	0	1	0	5
100.0	20	18	20	90	100

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 24-hour EC50 of the test item was calculated to be 75.2 mg test item/L with 95 % confidence limits from 57.4 to 98.4 mg test item/L.
The 24-hour EC0 was 10 mg test item/L, the EC100 was higer than 100 mg test item/L.
The 48-hour EC50 of the test item was calculated to be 63.9 mg test item/L with 95 % confidence limits from 55.5 to 73.5 mg test item/L.
The 48-hour EC0 and the 48-hour NOEC was 10 mg test item/L, sice no significant immobilization rate and no other signs of intoxication were
observed at the test animals up to and including this test concentration.The 48-hour EC100 amounted to 100 mg test item/L.