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EC number: 270-336-2 | CAS number: 68425-16-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 September - 28 September 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Non GLP, guideline not stated, available as unpublished report.
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The inhibitory effect of polysulfide di-tert-dodecyl was tested by comparing cell growth of Pseudomonas putida strain in the test substance against controls. The test substance is insoluble in water and three possibilities for testing were considered: by adding direct product due in mid-cultivation; by dispersing the product in water using an ultrasonic probe; by dissolving the product in a third solvent (Dimethylformamide) miscible with water. The biomass produced was determined by turbidimetric measure after an incubation period of 16 ± 1 hour at 21 ± 1°C.
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data reported - Analytical monitoring:
- yes
- Details on sampling:
- The cell density (biomass Product-) was determined after an incubation period of 16 ± 1 hours.
- Vehicle:
- no
- Details on test solutions:
- Since polysulfide di-tert-dodecyl was insoluble in water, 3 methods of introduction of polysulfide di-tert-dodecyl were used:
1- polysulfide di-tert-dodecyl was introduced directly in 100 mL of test medium by fractions from 10 mg to 1000mg. Flasks were placed under agitation during 16 hours at 21°C.
2- Formation of initial emulsion 5g/L of deionised water; by ultra sounds. Different dilutions of this emulsion were prepared until 1000mg/L. Flasks were placed under agitation during 16 hours at 21°C.
3- Dissolution of polysulfide di-tert-dodecyl in DMF(diméthylformamide) forming an initial solution at 5g/L. In the different dilutions of this initial solution, the limit of DMF must be < 0.2% , above this limit the DMF have an inhibitory effect > 10% to bacteria. Flasks were placed under agitation during 16 hours at 21°C. - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- A preculture was seeded by a bacterial suspension on levied miliu agar (under the age of 7 days) and incubated for 7 hours to obtain an exponential growth phase. The bacterial suspension with a turbidity of 30 to 60 NTU thus obtained served as inoculum for seeding the test medium.
- Test type:
- not specified
- Water media type:
- freshwater
- Total exposure duration:
- 16 h
- Remarks on exposure duration:
- Sterile water was used
- Post exposure observation period:
- No data reported
- Hardness:
- No data reported
- Test temperature:
- 21˚C
- pH:
- 7.2 + 0.2
- Dissolved oxygen:
- No data reported
- Salinity:
- No data reported
- Nominal and measured concentrations:
- 0; 10; 13; 17; 22; 29 and 37 mg/L.
- Details on test conditions:
- Orbital shaker flasks (100 rpm) incubated in the dark, thermostated at 21 ± 1°C, for 16 ± 1 hours. Inoculum was obtained from a preculture in logarithmic phase of coissance, having reached a turbidity of 30 to 50 NTU. Concentration 'test: maximum dose tested polysulfide di-tert-dodecyl: 10g/L (10,000 mg/L). Measurement of bacterial biomass: by turbidimetry expressed in NTU (nephelometric turbidity units). Controls used but unclear whether replicates used.
- Reference substance (positive control):
- yes
- Remarks:
- 3, 5 dichlorophenol
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- not specified
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Solvent added
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 g/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- not specified
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Ultrasonic method
- Details on results:
- Direct addition to the middle culture: In general, turbidity was of same order or higher than those in the controls. The product tended to form oily globules in the bottom of the flask and adsorbed on the wall when in turmoil. It has not been possible to determine an inhibitory effect up to a dose of 10 g/L for which it was found that bacterial growth was superior to that of controls.
Ultrasonic dispersion: The turbidity of the emulsion obtained at the limit of testing reads more or less as random. Emulsion diluted in the test medium and put into agitation for 16 hours seems to have been stable (formation of oily droplets, adsorption on the wall), and yet an inhibitory effect was not observed under a concentration of 1000 mg/L.
Dissolution in dimethylformamide: The solution was added into the middle of culture. The test was limited by turbidity characteristic of the product and the contribution of the solvent, with the maximum concentration tested being 10 m/L. A significant inhibitory effect was not observed. - Results with reference substance (positive control):
- No data reported
- Reported statistics and error estimates:
- No data reported
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The toxicity of polysulfide di-tert-nonyl to micro-organisms has been read across from polysulfide di-tert-dodecyl. The study attempts to test an insoluble substance in different types of solutions. No effect concentrations ranged 10mg/L (solvent added) - 10000 mg/L (ultrasonic method); direct addition of polysulfide in the GST middle culture did not determine an inhibitory effect up to a dose of 10 g/L but found that bacterial growth was superior to that of controls.
- Executive summary:
The toxicity of polysulfide di-tert-nonyl to micro-organisms has been read across from polysulfide di-tert-dodecyl. Hervouet (1989) is 16 hour, freshwater study on Pseudomonas putida following the method ISO/TC 147/SC 5 WG 1(N111). Due to the low solubility of the substance, several solutions were prepared, leading to a range of No Effect Concentrations from 10mg/L to 10 g/L. The study is considered suitable for use for this endpoint.
Reference
Description of key information
The toxicity of polysulfide di-tert-nonyl to micro-organisms has been read across from polysulfide di-tert-dodecyl. The study attempts to test an insoluble substance in different types of solutions. No effect concentrations ranged from 10mg/L (solvent added) to 10000 mg/L (ultrasonic method); direct addition of polysulfide in the GST middle culture did not determine an inhibitory effect up to a dose of 10 g/L but found that bacterial growth was superior to that of controls.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 10 g/L
Additional information
The toxicity of polysulfide di-tert-nonyl to micro-organisms has been read across from polysulfide di-tert-dodecyl. Hervouet (1989) is 16 hour, freshwater study on Pseudomonas putida following the method ISO/TC 147/SC 5 WG 1(N111). Due to the low solubility of the substance several solutions were prepared, leading to a range of No Effect Concentrations from 10mg/L to 10 g/L. The study is considered suitable for use for this endpoint.
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