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EC number: 200-143-0 | CAS number: 52-51-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conducted in accordance with GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- yes (incl. QA statement)
- Vehicle:
- no
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- - Supplier: Earthworm farm Tacke GmbH, Broken-Burlo, Germany
- Age of the animals at the begin of exposure: ca 9 months
- Weight of animals at the begin of the exposure (mean value): 515 mg
- Choice of the test animals: animals with a weight of 500 - 600 mg and a clear visible clitellum were taken out of the storage container and used for
the test.
- Adaptation of animals: 24 h before begin of exposure in the test substrate without test substance - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 20.8 to 22.2 °C
- pH:
- 6.4
- Moisture:
- Substrate: 32.8 g/100 g of dry weight
- Details on test conditions:
- TEST SUBSTRATE
- 10 % sphagnum peat
20 % kaolin clay
69.7 % quarz sand
0.3% calcium carbonate
TEST SYTSEM
- Test vessels: 1 L glass vessels covered with a lid
- Test volume: about 750 g (wet test substrate)
- Loading: 10 animals / test vessel (earthworms will be introduced into the test vessel impartially)
- Replicates/test concentration: 4
- Total number of animals/test concentration: 40
- Feeding: none
FURTHER TEST CONDITIONS
- Illumination: continuous light (400 - 800 lux)
- Application: exposure via test substrate, according to the natural exposure of earthworms - Nominal and measured concentrations:
- Nominal test concentrations (mg/L): control, 12.8, 32, 80, 200, 500
- Reference substance (positive control):
- yes
- Remarks:
- 2-chloroacetamide
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: weight
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.8 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- 14 d LC0 = 12.8 mg/kg soil dw
14 d LC100 > 500 mg/kg soil dw
14 d LOEC = 32 mg/kg soil dw
- Further observations: at the test assays with the test concentrations of 500 mg/kg and 200 mg/kg a reduced activity of the earthworms was observed. Furthermore, by comparison with the other test concentrations, it seemed the earthworms at the test concentration of 500 mg/kg were skinnier. - Results with reference substance (positive control):
- LC50 = 12.4 mg/kg; this result was slightly below the expected range of 15 to 35 mg/kg soil. This however did not affect the validity of the present study.
Reference
Mortalities after 14 days:
--------------------------------------------------
Test Conc. (mg/kg artificial soil) /Mortality (%)
0 (control) 5% (2/40)
12.8 8% (3/40)
32 13% (5/40)
80 10% (4/40)
200 13% (5/40)
500 15% (6/40)
--------------------------------------------------
Body weight (differences between test initiation and test ending):
-------------------------------------------------------
Test Conc.(mg/kg) MWi(mg/worm) MWe (mg/worm) Di/e(%)
Control 510 (Ni=40*) 484 (Ne=38**) 5
12.8 515 (Ni= 40) 386 (Ne=37) 25
32 511 (Ni=40) 335 (Ne=35) 34
80 503 (Ni=40) 396 (Ne=36) 21
200 528 (Ni=40) 401 (Ne=35) 24
500 521 (Ni=40) 276 (Ne=34) 47
-------------------------------------------------------
MWi: mean weight at test initiation
MWe: mean weight at test ending Di/e: difference between test initiation and ending
Additional findings: At 200 and 500 mg/kg soil the earthworms showed a reducted activity. At the highest test concentration, the animals furthermore appeared to be skinnier when compared to the remaining test groups.
Reference substance: an LC50 of 12.4 mg/ kg soil was obtained for 2-Chloroacetamide.
Description of key information
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 500 mg/kg soil dw
- Long-term EC10, LC10 or NOEC for soil macroorganisms:
- 62.5 mg/kg soil dw
Additional information
The acute toxicity of Bronopol to earthworms was examined in a GLP guideline study with Eisenia foetida according to OECD 207 [BASF AG 2006]. Adult earthworms were exposed to Bronopol-treated artificial soil for 14 days. At test termination a LC50 > 500 mg/kg dry weight and a NOEC = 12.8 mg/kg dry weight was determined.
The chronic toxicity of Bronopol to earthworms was examined in a GLP guideline study with Eisenia foetida according to OECD 222 [BASF SE 2021]. Adult earthworms were exposed to Bronopol-treated artificial soil for 28 days to investigate lethal effects resulting in an EC10 of 618.3 mg/kg (dw). The adult worms were removed thereafter and the reproduction by count of juvenile worms was investigated in the following. After 56 days a EC10 of 61.5 mg/kg (dw) regarding the reproduction was observed.
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