2-Thiophenecarboxylic acid, 3-amino-2,5-dihydro-4-methyl-, methyl ester, 1-oxide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-Thiophenecarboxylic acid, 3-amino-2,5-dihydro-4-methyl-, methyl ester, 1-oxide

EC Number:

603-029-2

Cas Number:

125088-75-7

Molecular formula:

C7H11NO3S

IUPAC Name:

2-Thiophenecarboxylic acid, 3-amino-2,5-dihydro-4-methyl-, methyl ester, 1-oxide

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

The test medium of the only test concentration of nominal 100 mg test item/L was prepared by dissolving 40.0 mg of the test item homogeneously in 400 mL test water by intense stirring for 15 minutes. The test medium was prepared just before introduction of the Daphnia (= start of the test).

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The toxic effect of the test item Dihydrothiophene Ester Sulfoxide to Daphnia magna was assessed in a static limit test. The 48-hour NOEC was determined to be > 100 mg test item/L. The 48-hour LOEC was determined to be ≥ 100 mg test item/L and the 48-hour EC50 value was determined to be > 100 mg test item/L.

Executive summary:

The purpose of this study was to determine the influence of the test item Dihydrothiophene Ester Sulfoxide on the mobility ofDaphnia magna. Young Daphnia (< 24 hours old) were exposed in a static limit test for 48 hours to test water containing the test item at the concentration of nominal 100 mg test item/L. This limit test was performed in compliance with the test guidelines to demonstrate that the test item has no toxic effect on Daphnia up to at least this concentration.

The test method of application and the test speciesDaphnia magnaare recommended by the test guidelines.

The purpose of the analytical part of this study was to verify the concentration of the test item in the test medium.

This study encompassed two treatment groups (one test item concentration at nominal 100 mg/L and one control) each containing 20 individuals. The mobility of the Daphnia was determined in a static 48-hour test by visual observation after 24 and 48 hours.

After 48 hours of exposure no immobilisation of the test animals was observed in the control and the only test item concentration of 100 mg test item/L.

The quantification of the test item Dihydrothiophene Ester Sulfoxide was performed using liquid chromatography with MS/MS detection.

At the start of the test 89 % of the nominal test concentration was found (average of the only test concentration). After 48 hours test duration, 88 % of the nominal value was determined (average of the only test concentration). During the test the daphnids were exposed to a mean of 88 % of nominal.Therefore, all reported results refer to nominal concentration.

The toxic effect of the test item Dihydrothiophene Ester Sulfoxide to Daphnia magna was assessed in a static limit test. The 48-hour NOEC was determined to be > 100 mg test item/L. The 48-hour LOEC was determined to be ≥ 100 mg test item/L and the 48-hour EC50 value was determined to be > 100 mg test item/L.