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EC number: 849-166-3 | CAS number: 83841-00-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Three nominal concentrations were tested: 10, 100 and 1000 mg/L; single test vessels were tested for the two lower concentrations, and three replicates were tested for the concentration of 1000 mg/L. A solubility pre-experiment showed that the test substance was soluble at a concentration of at least 5 g/L in deionised water, before and after adjustment of the pH from 2.2 to 7.9. A stock solution with a concentration of 5 g/L was prepared and the pH was adjusted from 2.2 to 7.3 with a sodium hydroxide solution. The test solutions were then prepared from the stock solution by dilution with deionised water.
The positive reference substance 3,5-dichlorophenol was tested at concentrations of 3.2, 10 and 32 mg/L (one vessel per concentration). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
ARA Birs, Birsfelden, Switzerland
- Method of cultivation: wet sludge was suspended in chlorine free tap water to achieve a concentration of 3 g dry material per litre. During the holding period of one day before use, the sludge was fed wonce with 50 mL synthetic sewage per litre
- Preparation of inoculum for exposure: before use on the next day, the pH of the activated sludge inoculum was adjusted from pH 6.9 to pH 7.5 with a 1M NaOH solution
- Initial biomass concentration: 3 g dry material per litre - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19 to 21 °C
- pH:
- Control vessels: 7.4 to 7.5 at start and 7.9 to 8.0 at end
Test substance vessels: 7.4 to 7.5 at start and 7.9 to 8.1 at end
Reference substance vessels: 7.2 to 7.5 at start and 7.9 to 8..1 at end - Dissolved oxygen:
- Control vessels: 8.5 to 8.6 mg/L at start and 7.8 to 8.0 mg/L at end
Test substance vessels: 8.2 to 8.5 mg/L at start and 7.8 to 8.2 mg/L at end
Reference substance vessels: 8.4 to 8.6 mg/L at start and 8.4 to 8.9 mg/L at end - Nominal and measured concentrations:
- Test substance: 10, 100, 1000 mg/L
Positive control: 3.2, 10, 32 mg/L - Details on test conditions:
- Synthetic sewage feed was prepared to contain 16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.4 g CaCl2 x 2H2O, 0.2 g MgSO4 x 7H20 and 2.8 g K2HPO4 per litre. The pH of the solution was 7.2.
Control vessels were prepared by using 16 mL synthetic sewage feed and making up to 250 mL with ionised water. Then 250 mL activated sewage sludge concentration with a nominal concentration of 3 g dry material per litre were added.
The test vessels were prepared by diluting proper volumes of the stock solution with deionised water, then adding 16 mL synthetic sewage feed and 250 mL of the activated sewage sludge inoculum.
The positive control vessels were prepared by mixing appropriate aliquots of a 3,5-DCP stock solution at a concentration of 500 mg/L with deionised water, synthetic sewage feed and inoculum in the respective test vessels to obtain the desired test concentrations. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The specific respiration rates for the two blank control replicates prepared at the beginning of the exposure period were 36 and 34 mg O2 per dry weight of activated sludge per hour and for two blank controls prepared at the end of the test were 34 and 39 mg O2/gh. All four values are higher than the 20 mg O2/gh stipulated in the guidelines.
The resulting coefficient of variation of oxygen uptake rate in the control replicates has a value of 6% and is therefore, as stipulated in the guidelines, not more than 30%. - Results with reference substance (positive control):
- The 3-hour EC50 of the reference item 3,5-dichlorophenol was calculated to be 6.6 mg/L with 95% confidence limits from 6.2 to 6.9 mg/L. The 3-hour EC50 is within the guidelines-recommended range of 2 to 25 mg/L, confirming the suitability of the activated sludge used.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 3-hour NOEC for the inhibitory effect on the respiration of activated sludge inoculum in the study was 1000 mg/L.
- Executive summary:
The inhibitory effect of the substance on the respiration rate of aerobic wastewater micro-organisms of activated sludge was investigated under GLP in accordance with OECD TG 209 in a 3-hour respiration inhibition test. A range finding test using concentrations of 10, 100 and 1000 mg test substance/L was performed. Single test vessels were used for the two lower concentrations, and three test vessel replicates were used at 1000 mg/L. The positive reference substance 3,5-dichlorophenol was also tested under the same conditions, at concentrations of 3.2, 10 and 32 mg/L. Four blank controls, containing an equal volume of activated sludge and synthetic sewage medium but no test or reference substances, were included in the test. The vessels were prepared in this order: two blank controls, positive reference substance vessels (with ascending concentrations, one vessel per concentration), test substance vessels (with ascending concentrations), and finally two blank controls. The test temperature was between 19 and 21 °C, the pH value in all test vessels was between 7.2 and 7.5 at the start and between 7.9 and 8.1 at the end, and the dissolved oxygen concentration ranged from 8.2 to 8.6 mg/L at the start and 7.8 to 8.9 mg/L at the end. All validity criteria of the test were fulfilled: the specific respiration rate of the blank controls was between 34 and 39 mg oxygen per gram dry weight of sludge per hour (thus >20 mg O2/g.h), the coefficient of variation of oxygen uptake rate in the control replicates was 6% (thus <30%), and the 3-hour EC50 of the positive reference substance was 6.6 mg/L (thus in the acceptable range from 2 to 25 mg/L). The test substance had no statistically significant inhibitory effect on the respiration rate of the activated sludge. The 3-hour NOEC in this study was 1000 mg/L.
Reference
Table 1: Results of the study
Vessel no. |
Nominal test concentration (mg/L) |
Oxygen consumption rate |
Inhibition % |
pH values |
Oxygen concentration (mg O2/L) |
|||
R in mg O2/L.h |
Rs in mg O2/g.h |
start |
end |
start |
end |
|||
Control |
||||||||
1 |
0 |
54.74 |
36.49 |
|
7.5 |
7.9 |
8.5 |
8.0 |
2 |
0 |
50.86 |
33.91 |
|
7.5 |
7.9 |
8.5 |
8.2 |
11 |
0 |
50.77 |
33.85 |
|
7.4 |
8.0 |
8.6 |
8.1 |
12 |
0 |
57.90 |
38.60 |
|
7.4 |
8.0 |
8.5 |
7.8 |
Mean Standard deviation Coefficient of variation |
53.57 3.43 6.40% |
35.71 2.29 6.40% |
|
|
|
|
|
|
Test substance |
||||||||
6 |
10 |
53.45 |
35.63 |
0.2 |
7.5 |
8.0 |
8.4 |
7.9 |
7 |
100 |
50.56 |
33.71 |
5.6 |
7.5 |
7.9 |
8.2 |
8.0 |
8 |
1000 |
49.04 |
32.69 |
8.5 |
7.5 |
8.1 |
8.3 |
7.8 |
9 |
1000 |
49.75 |
33.17 |
7.1 |
7.4 |
8.1 |
8.5 |
8.2 |
10 |
1000 |
54.00 |
36.00 |
-0.8 |
7.4 |
7.9 |
8.5 |
7.8 |
Positive reference substance |
||||||||
3 |
3.2 |
38.10 |
25.40 |
28.9 |
7.2 |
8.1 |
8.6 |
8.4 |
4 |
10 |
19.91 |
13.27 |
62.8 |
7.5 |
8.0 |
8.5 |
8.5 |
5 |
32 |
6.00 |
4.00 |
88.8 |
7.4 |
7.9 |
8.4 |
8.9 |
Description of key information
3-hour NOEC = 1000 mg/L (GLP, OECD TG 209)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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