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EC number: 924-152-0 | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (2S)-2,5-diaminopentanoic acid
- EC Number:
- 924-152-0
- Molecular formula:
- Not applicable (UVCB substance)
- IUPAC Name:
- (2S)-2,5-diaminopentanoic acid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Colony forming units in the test vessel: approx. 10E7 - 10E8 CFU/L
- Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 43 mg/L
- Based on:
- test mat.
- Initial conc.:
- 10.1 other: mg C/L
- Based on:
- other: TOC
- Details on study design:
- Duration: 28 d
Application: Once at test start
Test vessels: 5000 mL, brown glass
Volume of the test medium: 3000 mL
Test medium: Mineral salts medium acc. to OECD 301 B / CO2 Evolution Test
Test temperature: Nominal 22 ± 2 °C, actual measured 21.5 – 23 °C
Dispersion treatment: Continuous stirring
Aeration: 30 - 100 mL/min
Photoperiod: Low light conditions (brown glass bottles)
Sampling frequency: 1, 4, 6, 8, 11, 14, 18, 21, 25, 28 and 29 days
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 2
- Sampling time:
- 1 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 49
- Sampling time:
- 6 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 69
- Sampling time:
- 14 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 73
- Sampling time:
- 21 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 75
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- Functional control: 61% after 6 days, 80% after 14 days, 82% after 21 days and 82% after 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item is classified as readily biodegradable within after 28 days and complying to the 10-d-window.
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