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EC number: 205-426-2 | CAS number: 140-66-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- In accordance with Regulation (EC) 1907/2006 Annex XI (1.5) and the relevant ECHA guidance documents, the substances detailed in the table below are grouped for the purposes of read across to reduce the need for unnecessary repeat testing on the basis that the substances are similar on the basis of a common functional groups.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.901 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.08 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Reported statistics and error estimates:
- - NOEC and LOEC determined by one-way analysis of variance (ANOVA)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The read across for 4-tert-octylphenol (CAS: 140-66-9); is based upon the analogous substances to which basic form, degree of substitution of functional groups is not considered to effect the proposed read across for the endpoint of Toxicity to aquatic plants other than algae. Based on the information available for the read across substances, the substance is expected to be toxic to aquatic plants other than algae.
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- not provided
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Klimisch 2b. Guideline study with acceptable restrictions which do not impair the overall conclusion from the data.
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM 1991. E1415-91 Standard Guide for Conducting Static Toxicity Tests with Lemna gibba.
- Deviations:
- yes
- Remarks:
- Test was performed with alternate species. Plants at 2-frond stage were used rather than three or four frond stage recommended by ASTM. ASTM requirements for duration, frond production, light intensity or uniformity were not met.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: stock solutions generated with a liquid/liquid equilibrator
- Controls: dilution water control - Test organisms (species):
- Lemna minor
- Details on test organisms:
- TEST ORGANISM
- Common name: common duckweed
- Source: ERL-D culture
- Age: two-frond stage
- Nutrients: supplied throughout test
ACCLIMATION
- Culturing media and conditions: grown with nutrient media in static culture chamber with weekly nutrient additions - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- - Total hardness: 175 +/- 28.7 mg/L as CaCO3
- Test temperature:
- - Temperature: 22.8 +/- 0.3 °C
- pH:
- - pH: 8.25 +/- 0.13
- Dissolved oxygen:
- - Dissolved O2: 7.6 +/- 0.6 mg/L
- Salinity:
- NA
- Nominal and measured concentrations:
- - Test concentrations: <0.088, 0.109, 0.375, 0.901, and 2.080 mg/L measured
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: 2.4 L test solution per chamber
- Type of flow-through: proportional mini-diluter system
- No. of colonies per vessel: 22 frond plants per exposure concentration
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: City of Superior, WI municipal water supply. Dechlorinated by passing through activated charcoal column and addition of sodium sulphite. Passed through cation exchange resin to remove heavy metals.
- Alkalinity: 105 +/- 22.6 mg/L as CaCO3
OTHER TEST CONDITIONS
- Light intensity and quality: did not meet ASTM
EFFECT PARAMETERS MEASURED
- Determination of frond number: manual counting daily - Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.901 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.08 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Reported statistics and error estimates:
- - NOEC and LOEC determined by one-way analysis of variance (ANOVA)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC for inhibition of frond development was 0.901 mg nonylphenol/L. The LOEC for inhibition of frond development was 2.08 mg nonylphenol/L. No further conclusions drawn.
- Executive summary:
Brooke (1993).EPA: Klimisch 2b.
In a 96 hour acute toxicity study, Lemna minor (ERD-L culture) was exposed to nonylphenol at final measured test concentrations of <0.088, 0.109, 0.375, 0.901, and 2.080 mg nonylphenol/L under flow through conditions following ASTM guidelines. This test did not meet all ASTM requirements for duration or frond development, and light intensity, but was conducted for a sufficient period of time to observe effects. The NOEC for inhibition of frond development was 0.901 mg nonylphenol/L. The LOEC for inhibition of frond development was 2.08 mg nonylphenol/L.
There were no compound related phytotoxic effects.
Brook (1993) is classified as acceptable and satisfies the guideline requirements for a supporting acute Lemna minor toxicity test. The NOEC and LOEC values reported in Brooke (1993) are not comparable toEC50and other point estimates of effects. The NOEC and LOEC information is provided to support the findings in key studies Scholz (1992) and Ward and Boeri (1990).
Results Synopsis
Test Organism: Lemna minor– two frond stage
Test Type: Flow through
96 hEC50: none 95% C.I. : none
96 h EC50/IC50: none 95% C.I. : none
NOEC: 0.901 mg nonylphenol/L Probit Slope: none
LOEC: 2.08mg nonylphenol/L
Endpoint(s) Effected: Frond development
Brook (1993 ) was selected as a supporting study because nonylphenol toxicity information is presented for a common aquatic plant species other than algae in addition to the algae Selenastrum capricornutum. Results of this study support the finding of Key Study Ward and Boeri (1990).
Referenceopen allclose all
Description of key information
The NOEC for inhibition of frond development was 0.901 mg nonylphenol/L. The LOEC for inhibition of frond development was 2.08 mg nonylphenol/L. No further conclusions drawn.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater plants:
- 0.901 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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