Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-943-8 | CAS number: 7779-88-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Repeated dose toxicity: other routes
Administrative data
- Endpoint:
- repeated dose toxicity: other route
- Remarks:
- oropharyngeal aspiration
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Reliability:
- other: not rated according to Klimisch et al.
- Rationale for reliability incl. deficiencies:
- other:
- Remarks:
- The references contained in this summary entry represent in vivo experiments with investigat ions on repeated dose toxicity (route: oropharyngeal aspiration) with very limited value for risk assessment purposes. The references do not fulfil the criteria for quality, reliability and adequacy of experimental data for the fulfilment of data requirements under REACH and hazard as sessment purposes (ECHA guidance R4 in conjunction with regulation (EC) 1907/2006, Annexes VIIX). The information contained therein were included for information purposes only.
Data source
Reference
- Reference Type:
- publication
- Title:
- Pulmonary and hemostatic toxicity of multi-walled carbon nanotubes and zinc oxide nanoparticles after pulmonary exposure in Bmal1 knockout mice
- Author:
- Luyts, K. et al.
- Year:
- 2 014
- Bibliographic source:
- Particle and Fibre Toxicology 11:61.
Materials and methods
- Principles of method if other than guideline:
- Luyts, K. et al. (2014):
Test substance: zinc oxide nanoparticles, (BASF Z-Cote; zinkite, uncoated, primary size: 100 nm, NM110) obtained from the European Commission Joint Research Centre Nanomaterials Repository; X-ray diffraction size: 70 to >100 nm (by TEM analysis); size: 20-250/50-350 nm; Brunauer-Emmett-Teller (BET) surface area: 14 m²/g
Doses/Concentrations: 6.4 or 12.8 μg per aspiration (concentrations: 256 or 512 μg/mL)
No. of animals per sex per dose: not specified
Exposure duration: 5 consecutive weeks
Exposure frequency: once a week
Negative control: sterile water containing 2 vol% mouse serum
Positive control: not specified
Method of aerosol generation: not specified
Observation period: not specified
Parameters investigated: analysis of the number of macrophages, neutrophils, eosinophils and lymphocytes in the broncho-alveolar lavage (BAL) fluid by the DiffQuick method (Medical Diagnostics,
Düdingen, Germany); GSSG/GSH measurements (reduced (GSH) and oxidized (glutathione disulfide, GSSG)) in the left lung by UPLC/MS-MS and LC/MS-MS analysis; cytokine measurements in the remaining lobes of the right lung using the MSD mouse pro-inflammatory 7-plex (IFN-γ, IL-1β, IL-6, IL-10, IL-12p70, KC/GRO, TNF-α); measurement of partial thromboplastin time (aPTT), the prothrombin time (PT) and plasma levels of coagulation factors (F) VII, FVIII and fibrinogen in the blood serum; histological examination of the superior lobe of the right lung and heart, analysis of the arc/bow of the aortic tissues by incubation with antibodies and the biotinyl tyramide kit.
Test material
- Reference substance name:
- Zinc oxide
- EC Number:
- 215-222-5
- EC Name:
- Zinc oxide
- Cas Number:
- 1314-13-2
- Molecular formula:
- OZn
- IUPAC Name:
- oxozinc
- Test material form:
- solid: nanoform
Constituent 1
Test animals
- Species:
- other: Luyts, K. et al. (2014): Bmal1−/− and Bmal1+/+ mice (C57BL/6 J)
- Sex:
- male/female
Administration / exposure
- Route of administration:
- other: Luyts, K. et al. (2014): oropharyngeal aspiration
Results and discussion
Effect levels
- Remarks on result:
- other:
- Remarks:
- Luyts, K. et al. (2014): ZnO NPs seemed to suppress the inflammatory (decreased neutrophils in Bmal1−/− mice) and oxidative response (increased total glutathione in Bmal1−/− mice), but were also procoagulant with a significant increase of the coagulation factor FVIII. The procoagulant effects, as well as the significant correlations between the pulmonary endpoints (inflammation and oxidative stress) and hemostasis parameters were more pronounced in Bmal1−/− mice than in Bmal1+/+ mice.
Applicant's summary and conclusion
- Conclusions:
- No conclusion can be drawn from the above publications due to lack of quality, reliability and adequacy of the experimental data for the fulfilment of data requirements under REACH.
The references contained in this summary entry represent in vivo experiments with investigations on repeated dose toxicity with very limited value for risk assessment purposes. All references do not fulfil the criteria for quality, reliability and adequacy of experimental data for the fulfilment of data requirements under REACH and hazard assessment purposes (ECHA guidance R4 in conjunction with regulation (EC) 1907/2006, Annexes VII-X). The information contained therein were included for information purposes only.
Luyts, K. et al. (2014):
The non-physiological route of administration via oropharyngeal aspiration is not guideline conform and not suitable to assess acute inhalation toxicity. Only two dose groups were tested which does not allow a dose-response related analysis. Furthermore, the number of test animals per sex per dose are not stated. Also, the experimental design is insufficiently documented (e. g. information about the test material (purity), test animals (initial body weight, acclimation period) and the environmental conditions (temperature, humidity)). Only selected parameters investigated (coagulation, inflammatory and oxidative stress parameters) and the investigated endpoints are not required for building an expert judgment and further assessment of the test substance under REACH (ECHA guidance R4 in conjunction with regulation (EC) 1907/2006, Annex VII-X).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)