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Diss Factsheets
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EC number: 231-218-6 | CAS number: 7450-69-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-06-01 - 2012-09-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to an internationally accepted guideline. All study parameters are based on the specific guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Phenyl diamidophosphate
- EC Number:
- 231-218-6
- EC Name:
- Phenyl diamidophosphate
- Cas Number:
- 7450-69-3
- Molecular formula:
- C6H9N2O2P
- IUPAC Name:
- [(diaminophosphoryl)oxy]benzene
- Test material form:
- solid: crystalline
- Details on test material:
- Name PPDA
Composition phosphoric acid phenylester diamide
CAS No. 7450-69-3
EINECS-No. 231-218-6
Molecular formula C6H9N2O2P
Molecular weight 172.1 g/mol
Purity 97.77% (HPLC)
Constituent 1
Test animals
- Species:
- other: human skin model EpiDermTM
Test system
- Type of coverage:
- open
- Vehicle:
- other: DPBS-buffer
- Amount / concentration applied:
- Tissue Amount
1 24.8 mg
2 25.0 mg
3 23.6 mg - Duration of treatment / exposure:
- 60 min.
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- other: other: % Formazan production
- Value:
- 93.8
- Remarks on result:
- other:
- Remarks:
- Basis: mean. Time point: 60 min. Max. score: 100.0. Reversibility: no data. (migrated information)
Any other information on results incl. tables
Designation |
PPDA |
Positive Control |
% Formazan production (Tissue 1) |
93.4% |
5.7% |
% Formazan production (Tissue 2) |
93.4% |
6.0% |
% Formazan production (Tissue 3) |
94.7% |
5.7% |
% Formazan production Mean |
93.8% |
5.8% |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The test item is considered not irritant.
- Executive summary:
One valid experiment was performed.
Three tissues of the human skin model EpiDermTMwere treated with PPDA for 60 minutes.
In average, 24.5 mg of the solid test item (wetted with 25 µL DPBS-buffer) were applied to each tissue and spread to match the tissue size (0.63 cm2; as indicated by supplier).
DPBS-buffer was used as negative control, 5% SDS-solution was used as positive control.
After treatment with the negative control, the absorbance values were within the required acceptability criterion of 1.0 < mean OD < 2.5. The positive control showed clear irritating effects. Variation within tissues was acceptable (< 18%).
After the treatment with the test item, the relative absorbance values were reduced to 93.8 %. This value is well above the threshold for irritation potential (50%). Therefore, PPDA is considered as
not irritant in the Human Skin Model Test.
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