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EC number: 201-166-9 | CAS number: 79-00-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: oral
Administrative data
- Endpoint:
- sub-chronic toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1985
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Study design comparable to guideline study , but without histopathological evaluation.
Data source
Reference
- Reference Type:
- publication
- Title:
- Toxicology of 1,1,2-trichloroethane in the mouse
- Author:
- White, K.L. Jr., Sanders, V.M., Barnes, D.W., Shopp, G.M.|Jr., Munson, A.E.
- Year:
- 1 985
- Bibliographic source:
- Drug Chem Toxicol., 8|(5): 333-355.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 408 (Repeated Dose 90-Day Oral Toxicity Study in Rodents)
- Deviations:
- yes
- Remarks:
- The test conditions during test and prior testing not indicated; no evaluation of the clinical signs and histopathological findings were not reported.
- Principles of method if other than guideline:
- Method: other: not mentioned (described in White and al. Immunotoxicological investigations in the mouse. General approach and methods, Drug Chem. Toxicol., (1985))
- GLP compliance:
- no
- Limit test:
- yes
Test material
- Reference substance name:
- 1,1,2-trichloroethane
- EC Number:
- 201-166-9
- EC Name:
- 1,1,2-trichloroethane
- Cas Number:
- 79-00-5
- Molecular formula:
- C2H3Cl3
- IUPAC Name:
- 1,1,2-trichloroethane
- Details on test material:
- Name of the test substance (as cited in the test report) : 1,1,2-trichloroethane.
- Analytical purity: 95%
- Stability under test conditions: no stabilizers were present with this chemical
- Storage condition of test material: no preservatives were present with this chemical
- Batch number: 070177 and 061197
- Source: Aldrich Chemical Co.
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- no data
Administration / exposure
- Route of administration:
- oral: drinking water
- Vehicle:
- water
- Details on oral exposure:
- Test substance was diluted in deionised water to achieve the desire concentration. The solutions were maintained in amber-coloured bottles with stainless-stell pouts fitted through cork stoppers. bottles were changed twice weekly.
- Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- Less than 10% of test substance was lost during the 3 or 4 days between bottle changes, as measured by GLC with head space analysis.
- Duration of treatment / exposure:
- 90 days
- Frequency of treatment:
- Continuously
Doses / concentrations
- Remarks:
- Doses / Concentrations:
20, 200, 2000 mg/l (calculated daily doses, female mice: 3.9; 44; 384 mg/kg/day; male mice: 4.4; 46; 305 mg/kg/day)
Basis:
nominal in water
- No. of animals per sex per dose:
- 32/dose/sex, except control group: 48/dose/sex
- Control animals:
- yes
- Details on study design:
- - Dose selection rationale: based on the results of the 14-day gavage study (White et al. 1985)
- Positive control:
- no
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: No data
DETAILED CLINICAL OBSERVATIONS: No data
BODY WEIGHT: Yes
- Time schedule for examinations: approximately twice weekly
WATER CONSUMPTION AND COMPOUND INTAKE (if drinking water study): Yes
- Time schedule for examinations: twice weekly
OPHTHALMOSCOPIC EXAMINATION: No data
HAEMATOLOGY: Yes
- Time schedule for collection of blood: 90 days
- How many animals: 14-16 per dose group, 23-24 in control
- Parameters examined: hematocrit, haemoglobin, leucocytes, erythrocytes, platelets, fibrinogen, prothrombin time, activated partial prothrombin time
CLINICAL CHEMISTRY: Yes
- Time schedule for collection of blood: 90 days
- Animals fasted: No data
- How many animals: 14-16 per dose group, 24 in control
- Parameters examined: calcium, phosphorous, sodium, chloride, potassium, protein, glucose, , cholesterol, serum glutavic oxaloacetic transaminase (SGOT), serum alkaline phosphatase (SAP), lactate deshydrogenase (LDH), serum glutamic pyruvic transaminase (SGPT), blood urea nitrogen (BUN), creatinine, glutathione
URINALYSIS: No data
NEUROBEHAVIOURAL EXAMINATION: No data
OTHER: Hepatic microsomal activities,
Parameters examined at the end of the 90-d exposure in 8 mice per sex/group: microsomal proteins, cytochrome p450, cytochrome b5, aminopyrine N-demethylase, aminopyrine hygroxylase. - Sacrifice and pathology:
- Organ weight: brain, testes, liver, kidneys, spleen, lungs, thymus
- Other examinations:
- No data
- Statistics:
- -Statistical Evaluation:
If a one-way analysis of variance of the means showed treatment effects, a Duncan's Multiple Range Test was performed. Values which differ from vehicle control at p < 0.05 were considered statistically significant. Each of the values represents the mean +/-SE.
Results and discussion
Results of examinations
- Clinical signs:
- not specified
- Mortality:
- not specified
- Body weight and weight changes:
- effects observed, treatment-related
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- effects observed, treatment-related
- Ophthalmological findings:
- not specified
- Haematological findings:
- effects observed, treatment-related
- Clinical biochemistry findings:
- effects observed, treatment-related
- Urinalysis findings:
- not specified
- Behaviour (functional findings):
- not specified
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Gross pathological findings:
- not specified
- Histopathological findings: non-neoplastic:
- not specified
- Histopathological findings: neoplastic:
- not specified
- Details on results:
- - Body weight: strongly low body weight gain was observed in males at 2 mg/mL.
- Fluid consumption: there was a 30% decrease in fluid consumption in male mice drinking the highest concentration of 1,1,2-trichloroethane over the 90 day period. However, there was no 1,1,2-trichloroethane-related change in fluid consumption for the female mice.
- Organ weights:
* Males, there were no significant changes in weights for the brain, spleen, lungs, and thymus. On a mg basis, liver weights in male mice exposed to 0.2 and 2.0 mg/mL 1,1,2-trichloroethane were 11 and 12% smaller than the control group. This decreased liver weight was proportional to the decreased body weight; therefore, no significant changes could be measured on a percent of body weight basis. Similarly, kidneys weights were decreased of 12 and 16% on a mg basis for the groups receiving 0.2 and 2.0 mg/mL. Testicular weights of males exposed to the two highest concentrations increased 11 and 12% above control, but only on a percent of body weight basis
* Females responded differently in that the group receiving 2.0 mg/mL 1,1,2-trichloroethane had a 32% increase in absolute liver weight and a 26% increase when expressed as percent of body weight. Spleen and kidney weights of females exposed to the highest concentration were significantly increased on a mg basis only
- Hematology: there were few 1,1,2-trichloroethane exposure-related alterations in the hematological or blood coagulation parameters in the male mice . Male mice exposed to the highest concentration of 1,1,2-trichloroethane had decreased percent of polymorphonuclear leukocytes and increased percent of lymphocytes. Hemoglobin and hematocrit values of female mice exposed to 2.0 mg/mL 1,1,2-trichloroethane decreased by 6 and 5% (p < 0.05), as compared to the mice receiving deionized water. Erythrocytes also decreased, but not significantly. Platelet counts increased in all groups, but not in a dose-dependent fashion. Fibrinogen levels increased in females exposed to all three exposure levels, while prothrombin times decreased in the two highest exposure groups.
- Clinical chemistry: 14 serum chemistry parameters were evaluated. Some serum chemistry values were changed in males, which could be considered exposure-related regarding the 28% increase in cholesterol levels and the 61% increase in SAP activity in mice exposed to the highest concentration of 1,1,2-trichloroethane. The exposure-related alterations in the females included a 36% increase in cholesterol levels and a 63% increase in SGPT activity at the highest dose level, and SGOT and SAP increases at all dose levels (not dose dependent). Liver glutathione levels decreased dose dependently in males exposed to the two highest concentrations of 1,1,2-trichloroethane by 16 and 28% . Female mice exposed to the highest concentration of 1,1,2-trichloroethane showed a 13% increase in glutathione levels.
- Hepatic microsomal activities: In males, there were no perturbations of microsomal protein, cytochrome p-450 and b5 content. Microsomal enzymes that demethylate aminopyrine or hydroxylate aniline were not affected . Changes did occur with some of these parameters in the female mice. Cytochrome p-450 content and aniline hydroxylase activity were reduced dose dependently, while microsomal protein, cytochrome b5, and aminopyrine demethylase activity were unaffected.
Effect levels
open allclose all
- Dose descriptor:
- NOAEL
- Effect level:
- 3.9 mg/kg bw (total dose)
- Sex:
- female
- Dose descriptor:
- NOAEL
- Effect level:
- 4.4 mg/kg bw (total dose)
- Sex:
- male
- Dose descriptor:
- LOAEL
- Effect level:
- 44 mg/kg bw (total dose)
- Sex:
- male/female
- Basis for effect level:
- other: In females, reduction of cytochrome P450 content and aniline hydrolase activity. in males, reduction of liver glutathione
Target system / organ toxicity
- Critical effects observed:
- not specified
Applicant's summary and conclusion
- Conclusions:
- In a 90-day drinking water study with mice at concentrations of 0, 20, 200 or 2000 mg/L, NOAEL was considered to be 3.9 mg/kg/day (corresponding to 20 mg/L), and targen organ was determined to be the liver.
- Executive summary:
A 90 day drinking water study was performed, in which mice were administered test substance at 4.4, 46, and 305 mg/kg for males and 3.9, 44, and 384 mg/kg for females. The liver was determind to be the target organ in both sexes as demonstrated by dose-dependent alterations in hepatic microsomal enzyme activities and serum enzyme levels. The erythroid element of the female mice was also affected, as indicated by significantly decreased hematocrit and hemoglobin levels. The lowest effect level seen in females was 0.2 mg/mL (44 mg/kg), which resulted in a reduction of cytochrome p-450 levels and aniline hydroxylase activity. The no adverse effect level was 0.02 mg/mL (3.9 mg/kg) in females. In males, the lowest effect level was 0.2 mg/mL (46 mg/kg), which resulted in reduction of liver glutathione, and the no adverse effect level was 0.02 mg/mL (4.4 mg/kg).
NOAEL was derived at 3.9 mg/kg bw/d in females and 4.4 mg/kg bw/d in males, corresponding to 20 mg/L in drinking water.
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