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EC number: 205-355-7 | CAS number: 139-13-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- genetic toxicity in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Referenceopen allclose all
- Title:
- No information
- Author:
- IARC
- Year:
- 1 999
- Bibliographic source:
- IARC NTA mono73-19 (1999)
- Title:
- No information
- Author:
- Anonymous
- Year:
- 2 000
- Bibliographic source:
- IUCLID4 data set for trisodiumnitrilotriacetate, 18 February 2000, page 173-217 and 260-261
Materials and methods
Test guideline
- Guideline:
- other: see summary
- GLP compliance:
- not specified
- Type of assay:
- other: a number of different in vitro and in vivo tests
Test material
- Reference substance name:
- Nitrilotriacetic acid
- EC Number:
- 205-355-7
- EC Name:
- Nitrilotriacetic acid
- Cas Number:
- 139-13-9
- Molecular formula:
- C6H9NO6
- IUPAC Name:
- 2-[bis(carboxymethyl)amino]acetic acid
Constituent 1
Results and discussion
Test results
- Species / strain:
- other: see summary below
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The IARC monograph on nitrilotriacetic acid (volume 73, chapter 19) from 1999, which is attached in section 13, summarizes the genotoxic effects as follows (see also table 1 of the monograph). The cytotoxicity studies can be found on page 260 -261 of the IUCLID4 data set which is attached in section 13, the genotoxicity studies on page 173 -217.
Nitrilotriacetic acid did not induce reverse mutation in Escherichia coli and did not
induce gene mutation in either Saccharomyces cerevisiae or Schizosaccharomyces pombe.
It did not induce sex-linked recessive lethal mutation or dominant lethal mutation but
induced aneuploidy in Drosophila melanogaster. It did not induce sister chromatid
exchange or chromosomal aberrations in Chinese hamster cells in vitro. It did not induce
dominant lethal mutation but induced aneuploidy in mice in vivo.
Nitrilotriacetic acid, disodium salt did not induce gene mutation in mouse lymphoma
L5178Y tk+/– cells or sister chromatid exchange in Chinese hamster lung V79 cells
in vitro. It did not induce oxidative DNA damage in rat kidney cells in vivo.
Nitrilotriacetic acid, trisodium salt gave negative results in the bacterial SOS DNA
repair assay but induced differential toxicity in various repair-deficient Escherichia coli
WP2 strains. It did not induce gene mutation in Salmonella typhimurium TA100, TA1535,
TA1537, TA1538 or TA98 or in E. coli WP2 uvrA with or without exogenous metabolic
activation. It did not induce gene conversion, crossing-over, forward mutation or aneuploidy
in yeast and fungi without exogenous metabolic activation or gene conversion or
forward mutation with exogenous metabolic activation. Nitrilotriacetic acid, trisodium salt
induced micronuclei and chromosomal aberrations in plant cells, but it did not give rise to
micronuclei in Chinese hamster lung cells in vitro. It weakly induced somatic mutation in
D. melanogaster. It did not induce unscheduled DNA synthesis in rat primary hepatocytes
in the absence of metabolic activation. Nitrilotriacetic acid, trisodium salt did not induce
gene mutation at the hprt locus of Chinese hamster lung V79 cells without exogenous
metabolic activation or at the tk locus of mouse lymphoma L5178Y cells with or without
exogenous metabolic activation. It did not induce sister chromatid exchange in Chinese
hamster ovary cells or mouse lymphocytes in vitro. Nitrilotriacetic acid, trisodium salt
induced chromosomal aberrations in rat-kangaroo kidney cells in vitro without exogenous
metabolic activation and gene mutation in human cells in vitro. It did not induce sister
chromatid exchange or chromosomal aberration in human lymphocytes in vitro. It did not
induce micronuclei or aneuploidy in vivo in mice treated with a single intraperitoneal
injection.
No data were available on the genetic and related effects of nitrilotriacetic acid or its
salts in humans. Nitrilotriacetic acid and its disodium and trisodium salts were not genotoxic
in experimental systems in vivo, except that the acid induced aneuploidy in mouse
germ cells. Neither the acid nor its salts were genotoxic in mammalian cells in vitro and
they were not mutagenic to bacteria.
Urothelial cytotoxicity and regenerative hyperplasia were seen in male and female rats but not in mice,
and only at doses higher than those that produced nephrotoxicity. The mechanism is unclear but appears to
involve cellular Ca++ depletion secondary to the chelating effect of nitrilotriacetic acid. Urinary microcrystals were also produced.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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