Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 206-761-7 | CAS number: 373-02-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for publication.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Nickel (II)-induced cytotoxicity and apoptosis in human proximal tubule cells through a ROS- and mitochondria-mediated pathway
- Author:
- Wang YF, Shyu HW, Chang YC, Tseng WC, Huang YL, Lin KH, Chou MC, Liu HL, Chen CY.
- Year:
- 2 012
- Bibliographic source:
- Toxicology and Applied Pharmacology; 259:177-186
Materials and methods
- Type of study / information:
- Cell culture study to evaluate cytotoxicity and apoptosis.
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Human proximal tubule epithelial cells were treated with nickel acetate for up to 72 hours and then evaluated for the following: cell viability, colony formation, reactive oxygen species (ROS), DNA damage, mitochondrial membrane potential, apoptosis, cytochrome c concentration, and various protein levels.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Nickel di(acetate)
- EC Number:
- 206-761-7
- EC Name:
- Nickel di(acetate)
- Cas Number:
- 373-02-4
- Molecular formula:
- Ni(CH3CO2)2
- IUPAC Name:
- nickel di(acetate)
Constituent 1
- Specific details on test material used for the study:
- As cited in paper: nickel (II) acetate
Results and discussion
Any other information on results incl. tables
Nickel-treated cells had significant reduction in cell viability in a concentration-dependent manner. Cell viability was less than 50% after 48 hours when the highest dose of nickel (480 uM) was used. Colony formation was suppressed by 50 -75% at all nickel concentrations as compared to control.
Nickel-treated cells had significant increase in DNA damage as observed from the comet assay. Intracellular ROS significantly increased after treatment for 48 hours. At the highest dose (480 uM) after 48 hours, a 208% increase was observed as measured by DCF fluourescent intensity.
Cell mitochondrial membrane potential (MMP) was reduced (as measured by reduced Rh123 staining) following nickel-treatment.
Nickel-treatment also increased apoptosis (as measured by the percent of cells in sub-G1 phase). Control had 2.15% and treated cells to 19.39% for 160 uM nickel acetate, 28.32% for 320 uM nickel acetate, and 45.21% for 480 uM nickel acetate.
Nickel-treatment significantly reduced levels of Bcl-2 and Bcl-xL proteins (which regulate the mitochondrial apoptotic pathway). However, nickel-treatment increased levels of Bad, Bcl-Xs, and Bax proteins in a concentration-dependent manner.
Nickel-treatment resulted in a significant increase in cytosolic cytochrome c, but a decrease in mitochondrial cytochrome c (indicating a dose-dependent release of cytochrome c). This is typically associated with reduction of MMP (as reported above). Also caspases were significantly activated compared to control cells.
Applicant's summary and conclusion
- Executive summary:
Study was rated by an independent reviewer.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.