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EC number: 204-886-1 | CAS number: 128-44-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of test chemical
- Justification for type of information:
- Experimental data of test chemical is from handbooks and peer reviewed journal
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- WoE report is prepared based on toxicity to microorganism studies:
1, 2, 3 and 4th study - GLP compliance:
- no
- Analytical monitoring:
- not specified
- Details on sampling:
- 1st study: - Concentrations: 4 g L−1
- Sampling method: A synthetic feed composed of easily available nutrients was fed to activated sewage sludge together with the test substance or reference substance (3,5 dichlorophenol) and the mixture was aerated for 30 min.
- Sample storage conditions before analysis: No data available - Vehicle:
- yes
- Details on test solutions:
- 1. PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: Feed
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Synthetic feed composed of easily available nutrients - Test organisms (species):
- other: 2. activated sludge of a predominantly domestic sewage, 3, 4. Unadapted biological waste water treatment plants, 5. Tetrahymena pyriformis
- Details on inoculum:
- 1. - Laboratory culture: No
- Method of cultivation: Municipal sludge was obtained from the aeration tanks of wastewater treatment plants treating mostly household sewage.
- Preparation of inoculum for exposure: Test mixture was transferred to Karlsruhe bottles, an oxygen electrode was inserted to prevent headspace formation over the liquid. The oxygen content was recorded for 10 min.
- Pretreatment: No data available
- Initial biomass concentration: No data available - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 17 h
- Post exposure observation period:
- 2nd study: 10 min
5th study: 60.0 hours - Details on test conditions:
- 1. TEST SYSTEM
- Test vessel: Karlsruhe bottles
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: An oxygen electrode was inserted to prevent headspace formation over the liquid.
- Aeration: Yes, for 30 min
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: No data available
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal sludge was obtained from the aeration tanks of wastewater treatment plants treating mostly household sewage in Delmenhorst (Germany).
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Respiration inhibition were observed - Reference substance (positive control):
- yes
- Remarks:
- 1. 3,5-dichlorophenol
- Duration:
- 10 min
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: 1st study
- Duration:
- 17 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 2nd study
- Duration:
- 17 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3 800 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 3rd study
- Key result
- Duration:
- 60 h
- Dose descriptor:
- IC50
- Effect conc.:
- 387.7 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 5th study
- Reported statistics and error estimates:
- 1. Statistical analysis was performed by using Shapiro–Wilk and Bartlett tests to confirm normality and equal variance, respectively. For those data, which met these criteria, a one way analysis of variance (ANOVA) was performed to identify differences between treatment levels and controls.
- Validity criteria fulfilled:
- no
- Conclusions:
- 1. NOECs was considered to be 1000 mg/L when Activated Sludge was treated with test chemical for 10 minutes.
2. Based on the 17 hrs exposure study of bacteria with the test chemical, the EC50 was determine to be 7000 mg/l on the basis of growth rate inhibition.
3. Based on the growth rate inhibition of bacteria by the test chemical for 17 hrs, the EC50 was 3800 mg/l.
4. Inhibition concentration IC50 to 50% of Tetrahymena pyriformis when expeosed to test chemical for 60 h is 387.7 mg/L. - Executive summary:
Various studies available for the test chemical were reviewed to determine the toxic nature of test chemical on the growth and other activity of microorganisms. The studies are as mentioned below:
In a Respiration Inhibition Test, Activated Sludge treated with test chemical in the synthetic feed composed of easily available nutrients for 10 min. no effect were observed on respiration rate of treated Activated Sludge as compared to control. Therefore, NOECs was considered to be 1000 mg/L when Activated Sludge were treated with test chemical.
First study supported by second study. Study was carried out to evaluate the nature of chemical on the bacterial growth. Study was conducted for 17 hrs in which unadapted biological waste water treatment plants were exposed with chemical. Based on the 17 hrs exposure study of bacteria with the test chemical, the EC50 was determine to be 7000 mg/l on the basis of growth rate inhibition.
Aim of this next study was to determine the nature of test chemical on the growth of bacteria. Study was conducted for 17 hrs in which unadapted biological waste water treatment plants were exposed with chemical. Based on the growth rate inhibition of bacteria by the test chemical for 17 hrs, the EC50 was 3800 mg/l. Thus the chemical was not highly toxic to the growth of bacteria.
Similar study was carried out to detrmine the effects of test chemical on micro-orgnaisms test was carried out under static condition for 60 hours. Decreasing trend of Population growth rate was measured during the test. Inhibition concentration IC50 to 50% of Tetrahymena pyriformis when expeosed to test chemical for 60 h was determine to be 387.7 mg/L.
Thus based on the overall data for the test chemical, it was consider that the chemical was not toxic to microorganisms.
Reference
1. Obtained LOECs for target compounds towards investigated test systems.
LOEC [mg L−1] |
|
|
Activated sludge respiration inhibition |
Test chemical |
>1000 |
Description of key information
1. NOECs was considered to be 1000 mg/L when Activated Sludge was treated with test chemical for 10 minutes.
2. Based on the 17 hrs exposure study of bacteria with the test chemical, the EC50 was determine to be 7000 mg/l on the basis of growth rate inhibition.
3. Based on the growth rate inhibition of bacteria by the test chemical for 17 hrs, the EC50 was 3800 mg/l.
4. Inhibition concentration IC50 to 50% of Tetrahymena pyriformis when expeosed to test chemical for 60 h is 387.7 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 387.7 mg/L
Additional information
Various studies available for the test chemical were reviewed to determine the toxic nature of test chemical on the growth and other activity of microorganisms. The studies are as mentioned below:
In a Respiration Inhibition Test, Activated Sludge treated with test chemical in the synthetic feed composed of easily available nutrients for 10 min. no effect were observed on respiration rate of treated Activated Sludge as compared to control. Therefore, NOECs was considered to be 1000 mg/L when Activated Sludge were treated with test chemical.
First study supported by second study. Study was carried out to evaluate the nature of chemical on the bacterial growth. Study was conducted for 17 hrs in which unadapted biological waste water treatment plants were exposed with chemical. Based on the 17 hrs exposure study of bacteria with the test chemical, the EC50 was determine to be 7000 mg/l on the basis of growth rate inhibition.
Aim of this next study was to determine the nature of test chemical on the growth of bacteria. Study was conducted for 17 hrs in which unadapted biological waste water treatment plants were exposed with chemical. Based on the growth rate inhibition of bacteria by the test chemical for 17 hrs, the EC50 was 3800 mg/l. Thus the chemical was not highly toxic to the growth of bacteria.
Similar study was carried out to detrmine the effects of test chemical on micro-orgnaisms test was carried out under static condition for 60 hours. Decreasing trend ofPopulation growth rate was measured during the test.Inhibition concentration IC50 to 50% of Tetrahymena pyriformis when expeosed to test chemical for 60 h was determine to be 387.7 mg/L.
Thus based on the overall data for the test chemical, it was consider that the chemical was not toxic to microorganisms.
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