3-amino-2-chlor-6-methylphenol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Reference substance 001

Cas Number:

80419-48-3

Test material form:

solid

Details on test material:

beige powder

Specific details on test material used for the study:

Analogy
CAS 80419-48-3, 2-chloro-6-methyl-3-aminophenol -hydrochloride

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

Acclimatisation period: at least 5 days under housing conditions
Water: tap water ad libitum
Diet: ad libitum
Room temperature/relative humidity: approx. 20-25°C / 45 -60 %
Day/night cycle: 12 hours/12 hours
Fasting period:
Food deprivation: approx. 16 hours prior to and until 3 hours after application

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

single dose application, application volume of 20 ml/kg body weight

Doses:

group 1: 0.501 g/kg = 2.5%
group 2: 1.00 g/kg = 5.0%
group 3: 1.25 g/kg = 6.25%
group 4: 1.58 g/kg = 7.9%
group 5: 1.99 g/kg = 9.95%

No. of animals per sex per dose:

group 1, group 2, group 4, group 5 : 10 male rats
group 3: 20 male rats

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

1 360 mg/kg bw

Mortality:

deaths occured after 1-24 hours after application

Clinical signs:

other: above a dose of : 0.5 g/kg: apathetic, dizzily, accelerated breathing, later difficult respiration, prone position, urinary excretion (yellow-orange)

Any other information on results incl. tables

Mortality - male rats

group number	dose g/kg	number of animals	number of dead animals	number of animals with sypmtoms
1	0.501	10	0	10
2	1.00	10	1	10
3	1.25	20	9	20
4	1.58	10	8	10
5	1.99	10	9	10

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

Based on the given results 2 -chlor-6-methyl-3-aminophenol-hydrochloride has to be considered as harmful.

Executive summary:

The acute oral toxicity of 2 -chlor-6-methyl-3-aminophenol-hydrochloride was tested in young TNO - Wistar rats, supplier Winkelmann, Borchen. 10 - 20 male animals with a mean body weight of 200 g were used per dose. The test compound was administered by gavage in aqua dest. as solvent and an application volume of 20 ml/kg body weight.

The LD50 for male rats was 1.36 (1.21 - 1.54) g/kg.