Tetrahydrothiophene

Specific investigations: other studies

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biochemical or cellular interactions

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: data from iCSS CompTox Dashboard. Data Quality 100%. Data manually curated with highest confidence

Principles of method if other than guideline:

Using a high-throughput robotic screening system, THT was assessed for its potential to disrupt biological pathways (DNA binding, growth factor, nuclear receptor (non-steroidal and steroidal), cell cycle and cell morphology) that may result in toxicity.

Type of method:

in vitro

Specific details on test material used for the study:

Sample: TP0001179C11

Vehicle:

DMSO

Remarks:

1.25 to 200µM

Details on study design:

See enclosed excel file

Details on results:

242 assays were performed, tetrahydrothiophene did not induce a positive response. Therefore, there is no evidence that tetrahydrothiophene could interfere with the expression of the screened genes.

242 assays were performed, all results are displayed in the enclosed excel file.

Tetrahydrothiophene did not induce a positive response (see attached figure).

Executive summary:

Using a high-throughput robotic screening system, THT was assessed for its potential to disrupt biological pathways (DNA binding, growth factor, nuclear receptor (non-steroidal and steroidal), cell cycle and cell morphology) that may result in toxicity. THT did not induce a positive response in any of the 242 assays. Therefore, there is no evidence that THT could interfere with the expression of the screened genes.

Reference 2

Endpoint:

biochemical or cellular interactions

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Principles of method if other than guideline:

The PubChem BioAssay database was searched for chemogenomic, medicinal chemistry and functional genomics biological activities

Type of method:

in vivo

Specific details on test material used for the study:

PubChem CID: 1127
PubChem SID: 103463040 and 251919825

Details on results:

62 bioassays were retrieved from the PubChem BioAssay data base. THT was negative in 52 assays, inconclusive in 6 assays and 3 assays were unspecified. THT was reported as active in1 bioassays: AID 322956 (Inhibition of synthetic amyloid beta42 oligomer formation by Western blot), but it is a mistake because the tested compounds were derivatives of thioflavin-T (ThT) .

The list of all the assays performed is displayed in the enclosed excel file.

Executive summary:

The PubChem BioAssay database was searched for chemogenomic, medicinal chemistry and functional genomics biological activities. Sixty-one bioassays performed with THT were retrieved from the PubChem BioAssay data base. THT was negative in 52 assays, inconclusive in 6 assays and 3 assays were unspecified. THT had no activity on cell viability assays, no agonist and/or antagonist activities on the H2AX, AP-1, constitutive androstane receptor (CAR), hypoxia (HIF-1), RXR, thyroid stimulating hormone receptor (TSHR), androgen receptor (AR), estrogen receptor alpha (ER-alpha),retinoic acid receptor (RAR) and retinoid-related orphan receptor gamma (ROR-gamma) signaling pathways.

Description of key information

Using a high-throughput robotic screening system (US EPA, 2017), THT was assessed for its potential to disrupt biological pathways (DNA binding, growth factor, nuclear receptor (non-steroidal and steroidal), cell cycle and cell morphology) that may result in toxicity. THT did not induce a positive response in any assay. Therefore, there is no evidence that THT could interfere with the expression of the screened genes.

The PubChem BioAssay database was searched for chemogenomic, medicinal chemistry and functional genomics biological activities (NCBI, 2017). Sixty-one bioassays performed with THT were retrieved from the PubChem BioAssay data base. THT was negative in 52 assays, inconclusive in 6 assays and 3 assays were unspecified. THT had no activity on cell viability assays, no agonist and/or antagonist activities on the H2AX, AP-1, constitutive androstane receptor (CAR), hypoxia (HIF-1), RXR, thyroid stimulating hormone receptor (TSHR), androgen receptor (AR), estrogen receptor alpha (ER-alpha), retinoic acid receptor (RAR) and retinoid-related orphan receptor gamma (ROR-gamma) signaling pathways.