2-chloro-3-oxopentyl acetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

48 hours, February/March 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Test performed according to GLP rules and according to OECD guideline 202

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-chloro-3-oxopentyl acetate

EC Number:

235-930-8

EC Name:

2-chloro-3-oxopentyl acetate

Cas Number:

13051-49-5

Molecular formula:

C7H11ClO3

IUPAC Name:

2-chloro-3-oxopentyl acetate

Details on test material:

- Name of test material (as cited in study report): Chlorketone

- Physical state: liquid

- Lot/batch No.: average sample of 01199815, 01199905, 01200127,01200175

- Stability under test conditions: 8 - 15 months
- Storage condition of test material: keep away from oxidising agents

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: < -30° C
- Boiling point: 246 °C
- Vapour pressure: 1.3 mbar
- Water solubility (under test conditions): 1.0 mg/l

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

no data

Test solutions

Vehicle:

not specified

Details on test solutions:

test substance "not difficult"

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name:
- Strain: Strauss, Stamm Berlin
- Age at study initiation (mean and range, SD): 1- 24 h
- Method of breeding: EN IS0 6341
- Feeding during test
- Food type: Tetra AniminB, Scenedesmus subspicafus


ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): same
- Type and amount of food: -
- Feeding frequency: -
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

24 h

Post exposure observation period:

-

Test conditions

Hardness:

not recorded

Test temperature:

20+/- 2°C

pH:

7.6 - 8.4

Dissolved oxygen:

8.6 - 9.7

Salinity:

potable water contained: Ca2+ 31,6 mg/l, Mg2+ 5,5 mg/l, K+ 3,2 mg/l, Na+ 11,2 mg/l
added: CaCl2*2H20 177,9 mg/l, MgS04*7H20 67,4 mg/l, NaCl 17,8 mg/l

Nominal and measured concentrations:

1000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open

- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates):



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:


OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 / 8
- Light intensity:


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :


TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 76 - < 98 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL

Effect concentrations 2

Duration:

24 h

Dose descriptor:

NOELR

Effect conc.:

68 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

500 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: not found
- Observations on body length and weight: not recorded
- Other biological observations: not relevant

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50/LC50: 1.82 mg/l

Reported statistics and error estimates:

24 h EC50: 85 mg/l (95%: 76 - 98 mg/l)
24 h EC50 (ref): 1.82 mg/l (+- 0.03 mg/l)

Any other information on results incl. tables

no remarks

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 24 h EC 50 value is 85 mg/l (95% confidence limits: 76 - 98 mg/l)
NOEL (24h): 68 mg/l
EC 100 (24h): 500 mg/l

Executive summary:

A range-finding test was followed by a final EC50 test performed under static conditions for 24 hours of exposure. In the final EC50 test Daphnia were exposed for a maximum of 24 hours to 12 different concentrations

The study included a blank-control. The test was performed in quadruplicate with 5 daphnia

per vessel. The 24h EC50 was determined to 85 mg/l (95% range: 76 - 98 mg/l).

24 h EC50 (reference substance) = 1.82 mg/l