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EC number: 262-108-6 | CAS number: 60209-82-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 24 Feb 1997
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented study report which meets basic scientific principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
- Principles of method if other than guideline:
- HET-CAM assay with 6 eggs.
- GLP compliance:
- not specified
Test material
Constituent 1
Test animals / tissue source
- Species:
- other: Chicken
- Strain:
- other: White leghorn
- Details on test animals or tissues and environmental conditions:
- TEST EGGS
- Source: INRA-PAP, 37380 Nouzilly,France
- Age at study initiation: 10 d
- Weight at study initiation: 50 - 60 g
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 37 - 38
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: yes, negative control eggs treated with 0.9% NaCl solution, positive control eggs treated with 3% sodium lauryl sulfate
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied: 0.2 mL - Duration of treatment / exposure:
- 20 sec
- Observation period (in vivo):
- up to 5 min after exposure
- Number of animals or in vitro replicates:
- 6 eggs (test substance)
3 eggs (0.9% NaCl solution; negative control)
3 eggs (3 % sodium lauryl sulphate; positive control) - Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): 5 mL saline (37 °C)
- Time after start of exposure: 20 sec.
SCORING SYSTEM:
Observations were scored according to table 1.
Classification was done according to table 2
Results and discussion
In vitro
Results
- Irritation parameter:
- other: Primary irritancy index
- Run / experiment:
- mean out of 6 eggs after 30 sec, 2 min or 5 min
- Value:
- 0
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: scores depending on the time point of reaction for the first time.
- Other effects / acceptance of results:
- No further effects reported.
Any other information on results incl. tables
Table 3: Irritation scoring - results
|
Hyperaemia |
Hemorrhage |
Coagulation |
Summed scores |
Egg No. 1 |
0 |
0 |
0 |
0 |
Egg No. 2 |
0 |
0 |
0 |
0 |
Egg No. 3 |
0 |
0 |
0 |
0 |
Egg No. 4 |
0 |
0 |
0 |
0 |
The test substance had no potential to cause irritation in the HET-CAM test.
The negative control, 0.9% NaCL solution, tested in parallel, did not cause irritation in any of the eggs (IP-CAM = 0.0)
The positive control, 3% SDS, induced clear irritating phenomena on every membrane (hyperaemia and haemorrhage observed after 30 s in all 3 eggs, coagulation observed after 5 min in 1 egg). A primary irritation index, IP-CAM, of 13.7 was obtained.
Applicant's summary and conclusion
- Interpretation of results:
- other: not corrosive
- Conclusions:
- There is regulatory acceptance in the EU that a substance can be considered a severe eye irritant (Serious eye damage Category 1/R41) based on a positive result in one of the available in vitro test methods, e.g. the Hen's Egg Test on Chorioallantoic Membrane (HET-CAM) test. Negative in vitro corrosivity responses are not conclusive with respect to non-classification or classification as irritant (Category 2/R36) and shall therefore be subject to further evaluation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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