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Diss Factsheets
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EC number: 233-333-7 | CAS number: 10124-41-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Specific investigations: other studies
Administrative data
- Endpoint:
- specific investigations: other studies
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well-documented short publication. Supplementary data with respect to the mechanism of cell transformation.
Data source
Reference
- Reference Type:
- publication
- Title:
- Transformation of Syrian hamster embryo cells by sodium bisulfite.
- Author:
- DiPaolo, J.A.; et al.
- Year:
- 1 981
- Bibliographic source:
- Cancer Letters, 12, 203- 208
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Transformation of Syrian hamster embryo cells (HEC) by bisulfite and after treatment with UV irradiation. Two-day-old secondary hamster cultures
were treated 24 h after plating for 15 min with varying concentrations of sodium bisulfite in PBS (0, 1, 5, 10, or 20 mM). With this procedure, all bisulfite treatment was at neutral pH. After treatment incubation was for 6 more days before determination of cell survival of HEC and transformation. - GLP compliance:
- no
- Type of method:
- in vitro
- Endpoint addressed:
- carcinogenicity
Test material
- Reference substance name:
- Sodium hydrogensulfite
- EC Number:
- 231-548-0
- EC Name:
- Sodium hydrogensulfite
- Cas Number:
- 7631-90-5
- IUPAC Name:
- sodium hydrogen sulfite
- Reference substance name:
- sodium bisulfite
- IUPAC Name:
- sodium bisulfite
- Details on test material:
- - Name of test material (as cited in study report): sodium bisulfite
- Molecular formula (if other than submission substance): NaHSO3
- Molecular weight (if other than submission substance): 104.06 g/mol
- Physical state: solid
- Analytical purity: Fisher certified mreagent sodium bisulfite was purchased from Fisher Scientific Co., Fairlawn, NJ
Constituent 1
Constituent 2
Test animals
- Details on test animals or test system and environmental conditions:
- not applicable
Administration / exposure
- Details on exposure:
- - Two-day-old secondary hamster cultures were treated 24 h after plating for 15 min with varying concentrations of sodium bisulfite in PBS (0, 1, 5, 10, or 20 mM).
- With this procedure, all bisulfite treatment was at neutral pH.
- After treatment incubation was for 6 more days before determination of cell survival of HEC and transformation.
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
1 mM K2S2O5
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
5 mM K2S2O5
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
10 mM K2S2O5
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
20 mM K2S2O5
Basis:
nominal conc.
- No. of animals per sex per dose:
- not applicable
- Details on study design:
- - Fresh HEC cells from foetuses 13-14 days in gestation were used.
- Primary and subsequent passage cells were grown as monolayers in Petri dishes in complete medium at 37 °C in 11 % CO2 humified atmosphere.
- The medium consisted of Dulbecco’s modification of Eagle’Sminimal essential medium with 10 % foetal bovine serum supplemented with hypoxanthine and thymidine.
- In all experiments, 2-day old hamster cultures obtained by seeding 2.5 x 10E6 cell/100 mm-dish, were used.
- Fort he quantitative assay, 300 cells were co-cultivated with 6 x 10E4 X-irradiation killed HEC in 60 mm dishes.
- Cells cultures were treated 24 h after being seeded by first removing the medium and incubation 15 min with varying concentrations of sodium bisulfite in PBS. With this procedure, all bisulfite treatment was at neutral pH.
- After treatment, the bisulfite solution was removed, complete medium with 15 %foetal bovine serum was added and incubation was continued for an additional 6 days prior tot he staining of cells.
- Transformation and cell survival of HEC were determined in the same dishes. Colony morphology was determined with a microscope at x10 – x50. The non-transformed colonies had a regularly oriented arrangement of cells whereas the transformed ones exhibited a random criss-cross piling up of cells not observed in untreated controls.
- Transformation was calculated both per dish and relative to the number of colonies surviving. The cloning efficiency (CE) was determined by dividing the average number of colonies by the number of cells seeded per plate, and multiplying with 100.
- Cells were UV irradiated. For UV treatment the medium was removed and the cells were irradiated through a Permanox top and covered with PBS for 15 min.
- Fort he UV and bisulfite combinations the cells were treated with bisulfite in 1 mL PBS before and after irradiation. After final treatment, the PBS was replaced with complete medium.
Results and discussion
- Details on results:
- - Concentrations of sodium bisulfite between 1 to 20 mM that caused minimal toxicity caused a dose-dependent increase in the transformation of Syrian hamster embryo cells.
- Exposure of the cells to bisulfite (up to 10 mM) either before or after UV irradiation with 3 J/m2 did not result in an increased transformation frequency.
- The results suggest that bisulfite transformation of HEC may not occur by a mechanism involving mutation.
Applicant's summary and conclusion
- Conclusions:
- From this study it may be suggested that the transformation of HEC by bisulfite did not occur by a mechanism involving mutation.
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