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EC number: 433-480-9 | CAS number: 623-53-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Feb 13 - Feb 15, 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study was performed in compliance with the Good Laboratory Practice (GLP) regulations (revised in 1997, ENV/MC/CHEM(98)17). The method followed that described in the OECD Guidelines for Testing of Chemicals (Adopted: 4 April 1984) No 202 "Daphnia sp., Acute Immobilisation Test and Reproduction Test".
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- None
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 433-480-9
- EC Name:
- -
- Cas Number:
- 623-53-0
- Molecular formula:
- C4H8O3
- IUPAC Name:
- ethyl methyl carbonate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: A volume of test sample was diluted with acetone to give a final theoretical concentration of 20 mg/l.
- Sample storage conditions before analysis: n.a.
Test solutions
- Vehicle:
- no
- Details on test solutions:
i) Stock Solutions
a) CaC12.2H20 11.76 g/l
b) MgSO4.7H20 4.93 gll
c) NaHC03 2.59 g/l
d) KCI 0.23 g/l
For preparation, an aliquot (25 ml) of each of solutions a-d was added to each litre (final volume) of deionised water .
After preparation, the reconstituded water was aerated until the dissolved oxygen concentration was approximately air-saturation value.
- Hardness: about 14° dH (about 250 mg/L CaCO3)
- pH: 7.8 ± 0.2, after an aeration for 24 hours.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Adult Daphnia magna were maintained in polypropylene vessels containing approximately 2 litres of reconstituted water in a temperature controlled room at 21°C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods. Each culture was fed daily with a suspension of algae (Chlarella sp.). Culture conditions ensured that reproduction was by parthenogenesis. Gravid adults were isolated the day before initiation of the test, such that the young daphnids produced overnight were less than 24 hours old. These young were removed from the cultures and used for testing. The diet and diluent water are considered not to contain any contaminant that would affect the integrity or outcome of the study.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 14 ° dH (about 250 mg/L CaCO3)
- Test temperature:
- 21-22 °C
- pH:
- 7.8 +/- 0.2
- Dissolved oxygen:
- at least 87 %
- Salinity:
- see test solution details
- Nominal and measured concentrations:
- measured 100 mg/L
- Details on test conditions:
- 1) Range finding test
Dose level: 0.1, 1, 10, 100 mg/L
Number of daphnids: 40 (4 x 10)
Vessel: 300 mL glass vessel
Volume: 300 mL reconstituted water
Temperature: 21 °C
Photoperiod: 16 hours light and 8 hours darkness
Exposure. 48 h
Food during exposure: No
Aearation during exposure: no
Observation period: 24 and 48 hours
2) Main study
Dose level: 100 mg/L
Replicates: 4
Number of daphnids: 40 (4 x 10)
Vessel: 300 mL glass vessel
Volume: 300 mL reconstituted water
Temperature: 21 °C
Photoperiod: 16 hours light and 8 hours darkness
Exposure. 48 h
Food during exposure: No
Aearation during exposure: no
Observation period: 24 and 48 hours
GC Analysis: 0, 48 hours - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- There was no immobilisation in 40 daphnids exposed to a test concentration of 100 mg/l for a period .
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EC50 of greater than 100 mg/l. Correspondingly the No Observed Effect Concentration was 100 mg/l.
- Executive summary:
- Purpose
The purpose of this assay was to identify the aquatic toxicity potential of the test material in Daphnia magna to provide a rational basis for hazard estimation for the test item in aquatic environments
Study Design
Following a preliminary range-funding study, forty daphnids (4 replicates of 10 animals) were exposed to an aqueous solution of the test material at a concentration of 100 mg/l for 48 hours under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 24 and 48 hours.
Results
The 24 and 48-hour EC50 for the test material to Daphnia magna based on nominal test concentrations was greater than 100 mg/l and correspondingly the No Observed Effect Concentration was 100 mg/l at 24 and 48 hours.
Nominal concentration Immobilized/exposed daphnids [mg/L] 24 hours 48 hours 100 0/40 0/40
Analysis of the test solutions at 0 and 48 hours showed measured test concentrations to be near nominal and so the results are based on nominal test concentrations only.
24 h EC50 > nominal 100 mg/L
48 h EC50 > nominal 100 mg/L
24 h NOEC > nominal 100 mg/L
48 h NOEC > nominal 100 mg/L
Conclusion
The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been investigated and gave a 24- and 48-hour EC50 of greater than 100 mg/l. Correspondingly the No Observed Effect Concentration was 100 mg/l at 24 and 48 hours.
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