Dibutyltin dichloride

Short-term toxicity to aquatic invertebrates

Description of key information

The following studies have been submitted to address the short-term toxicity to aquatic invertebrates endpoint:
Hooftman RN and De Wolf JM (2003b). Dibutyldichlorostannane (CAS # 683-18-1): Static acute toxicity test with the crustacean species Daphnia magna. Testing laboratory: TNO, Project Organisation, Ecotoxicology, Utrechtseweg 48, P. O. Box 370, 3700 AJ Zeist, The Netherlands. Report no.: V2495/02. Owner company: Organotin Environmental Programme (ORTEP) Association, Stabilizer Task Force. Study number: 01-2495/02. Report date: 2003-08-15.
Hooftman RN and De Wolf JM (2004). Dibutyldichlorostannane (CAS # 683-18-1): Static acute toxicity test with the crustacean species Daphnia magna. Testing laboratory: TNO, Project Organisation, Ecotoxicology, Utrechtseweg 48, P. O. Box 370, 3700 AJ Zeist, The Netherlands. Report no.: V2495/02. Owner company: Organotin Environmental Programme (ORTEP) Association, Stabilizer Task Force. Study number: 01-2495/02. Report date: 2004-11-11.
Thom RM et al (1991). Static acute 48-hour toxicity test of dibutyltin dichloride (DBT) to oyster larvae. Testing laboratory: Batelle Pacific Northwest Laboratories, Marine Sciences Laboratory, 439 W. Sequim Bay Road, Sequim, Washington, 98382, USA. Report no.: 12716. Owner company: The consortium of TBT Manufacturers, Atochem North America, Inc. and Sherex Chemical Co., Inc., 900 First Avenue, P. O. Box 1536, King of Prussia, PA 19406-0018, USA. Report date: 1991-03-28.
Vighi M & Calamari D (1985). QSARs FOR ORGANOTIN COMPOUNDS ON DAPHNIA MAGNA. Chemosphere, Vol.14, No.11/12, pp 1925-1932.
The Hooftmann and De Wolf references have been allocated a Klimisch score of 2; the Thom et al (1991) and Vighi and Calamari (1985) have been allocated Klimisch scores of 3 and 4 respectively. The Hooftmann and De Wolf references have been determined to be the key studies for this endpoint.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.843 mg/L

Additional information

Hooftman RN and De Wolf JM (2003/2004) was the key study for fulfilment of the data requirement. The study was performed in compliance with GLP and to current internationally accepted guidelines (OECD 202 and EU Method C.2). The study was assigned a reliability score of 2 (reliable with restrictions) as there were unavoidable deficiencies present in the study in relation to the analytical methods employed in the study, however due to the physical properties of the substance, derivatisation is the most appropriate method available. The study was a static acute toxicity test with the crustacean species Daphnia Magna (TNO study number: 01-2495/02) the test material was found to have a 48h EC50 value of 0.843 mg.l^-1. Correspondingly the No Observed Effect Concentration was 0.44 mg.l^-1. A study was performed to assess the acute toxicity of the test material to Daphnia magna.The method followed that described in the OECD Guidelines for Testing of Chemicals (April 2004) No 202," Daphnia sp, Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No 440/2008.

 

Thom RM et al (1991) and Vighi M & Calamari D (1985) were provided as supporting studies and were assigned reliability scores of 3 and 4 respectively.

Thom RM et al (1991), In a Static acute 48-hour toxicity test of dibutyltin dichloride (DBT) to oyster larvae (BNW project number: 12716,) performed to the guideline EPA OPP 72-3 (Estuarine/Marine Fish, Mollusk, or Shrimp Acute Toxicity Test), the test material was determined to have an LC50 of 66.9µg/L and an EC50 of 55.5µg/L. The study was assigned a reliability score of 3 (not reliable) as the methodology contained deficiencies which were considered to have an impact on the accuracy of the test.

Vighi M & Calamari D (1985) was performed according to OECD 202 (and EU Method C.2), however the reporting of the study was insufficient for accurate assessment of the quality of the data, the study was therefore assigned a reliability score of 4 (not assignable). The study was a static acute toxicity test with the crustacean species Daphnia Magna. The test material was found to have an EC50 of 0.9 mg/l.