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Diss Factsheets
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EC number: 932-349-8 | CAS number: 8006-64-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Justification for type of information:
- Beta-Pinene is a main constituent of UVCB substance gum tupentine oil. Therefore, data on beta-pinene can be used for extrapolation to gum turpentine oil. See read-across justification document in section 13.
Cross-reference
- Reason / purpose for cross-reference:
- read-across source
Data source
Materials and methods
Test material
- Reference substance name:
- Pin-2(10)-ene
- EC Number:
- 204-872-5
- EC Name:
- Pin-2(10)-ene
- Cas Number:
- 127-91-3
- Molecular formula:
- C10H16
- IUPAC Name:
- 6,6-dimethyl-2-methylenebicyclo[3.1.1]heptane
- Reference substance name:
- p-mentha-1(7),2-diene
- EC Number:
- 209-081-9
- EC Name:
- p-mentha-1(7),2-diene
- Cas Number:
- 555-10-2
- Molecular formula:
- C10H16
- IUPAC Name:
- 3-isopropyl-6-methylenecyclohexene
- Reference substance name:
- 7-methyl-3-methyleneocta-1,6-diene
- EC Number:
- 204-622-5
- EC Name:
- 7-methyl-3-methyleneocta-1,6-diene
- Cas Number:
- 123-35-3
- Molecular formula:
- C10H16
- IUPAC Name:
- 7-methyl-3-methyleneocta-1,6-diene
- Reference substance name:
- Pin-2(3)-ene
- EC Number:
- 201-291-9
- EC Name:
- Pin-2(3)-ene
- Cas Number:
- 80-56-8
- Molecular formula:
- C10H16
- IUPAC Name:
- 2,6,6-trimethylbicyclo[3.1.1]hept-2-ene
- Reference substance name:
- Dipentene
- EC Number:
- 205-341-0
- EC Name:
- Dipentene
- Cas Number:
- 138-86-3
- Molecular formula:
- C10H16
- IUPAC Name:
- 4-isopropenyl-1-methylcyclohexene
- Reference substance name:
- Camphene
- EC Number:
- 201-234-8
- EC Name:
- Camphene
- Cas Number:
- 79-92-5
- Molecular formula:
- C10H16
- IUPAC Name:
- 2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane
- Reference substance name:
- 3,7,7-trimethylbicyclo[4.1.0]hept-3-ene
- EC Number:
- 236-719-3
- EC Name:
- 3,7,7-trimethylbicyclo[4.1.0]hept-3-ene
- Cas Number:
- 13466-78-9
- Molecular formula:
- C10H16
- IUPAC Name:
- 3,7,7-trimethylbicyclo[4.1.0]hept-3-ene
- Test material form:
- liquid
- Details on test material:
- Batch No.: EG12R
Purity: 95.8%
Name of test material (as cited in study report): BETA-PINENE
NOTE: Due to an industry habit inherited from the past, the test material was referenced as beta pinene whereas it was actually (-)-beta pinene.
Physical state: Colorless-slightly amber liquid
Storage condition of test material: 6 ± 3 °C, protected from light; kept under nitrogen
Expiration date: April 21, 2011
Constituent 1
impurity 1
impurity 2
impurity 3
impurity 4
impurity 5
impurity 6
Test system
- Vehicle:
- unchanged (no vehicle)
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- other: other: viability % (MTT assay)
- Value:
- 38.5
- Remarks on result:
- other:
- Remarks:
- Basis: mean. Time point: 15 min. Reversibility: no data. Remarks: ± 3.5. (migrated information)
Any other information on results incl. tables
Negative control (PBS+): mean OD = 0.821
Positive control (5% SDS): % viability (MTT assay) = 18.7 ± 3.0
Test item: % viability (MTT assay) = 38.5 ± 3.5
Table 1: MTT conversion assay in living epidermis
|
|
O.D. 1 |
O.D. 2 |
Mean |
Standard deviation |
Viability % |
Mean Viability % |
Standard deviation |
Negative control |
Epidermis 1 |
0.768 |
0.808 |
0.788 |
0.028 |
96.0 |
100 |
0.044 |
Epidermis 2 |
0.855 |
0.864 |
0.860 |
0.006 |
104.7 |
|||
Epidermis 3 |
0.819 |
0.811 |
0.815 |
0.006 |
99.3 |
|||
Positive control |
Epidermis 1 |
0.124 |
0.145 |
0.135 |
0.015 |
16.4 |
18.7 |
0.030 |
Epidermis 2 |
0.158 |
0.131 |
0.145 |
0.019 |
17.6 |
|||
Epidermis 3 |
0.191 |
0.172 |
0.182 |
0.013 |
22.1 |
|||
Test item |
Epidermis 1 |
0.359 |
0.293 |
0.326 |
0.047 |
39.7 |
38.5 |
0.035 |
Epidermis 2 |
0.301 |
0.267 |
0.284 |
0.024 |
34.6 |
|||
Epidermis 3 |
0.358 |
0.318 |
0.338 |
0.028 |
41.2 |
Applicant's summary and conclusion
- Interpretation of results:
- irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The percentage of viability is ≤ 50% and (-)-beta-pinene is classified as irritating to skin, R38, according to the criteria of Directive 67/548/EEC and in category 2 according to CLP Regulation (EC) N° 1272/2008.
- Executive summary:
An in vitro GLP study conducted with human epidermis model EPISKIN was performed to assess the irritancy potential of (-)-beta-pinene similarly to ECVAM protocol version 1.8 of February 2009. 10 µL of the test item was applied directly on 3 epidermis for 15 min. Positive control was 10 µL of 5% (w/v) SDS solution and negative control was 10 µL of PBS (each tested on 3 epidermis). MTT conversion assay was peformed to evaluate the percentage of cellular viability of the epidermis. Positive control had a percentage of cell viability of 18.7± 3.0 and test item had a percentage of cell viability of 38.5 ± 3.5. As the percentage of viability is ≤ 50%, the test item is considered to be irritating for skin.
Therefore, (-)-beta-pinene is classified as irritating to skin, R38, according to the criteria of Directive 67/548/EEC and in category 2 according to CLP Regulation (EC) n° 1272/2008.
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