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EC number: 300-346-5 | CAS number: 93925-43-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 - 17 Feb 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Food and Consumer Product Safety Authority (VWA), Utrecht, The Netherlands
- Analytical monitoring:
- no
- Remarks:
- No suitable analytical method could be established to quantify the test substance in test medium.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Preparation of test solutions started with individually prepared loading rates of 1.0, 10 and 100 mg/L applying a 1 h period of magnetic stirring. The obtained mixtures containing undissolved material were allowed to settle for a period of 40 min after which the Water Accommodated Fractions were siphoned off and used as test solutions.
- Eluate: no
- Differential loading: yes
- Controls: yes, test medium control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No, test solutions were clear and colorless. - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: carp
- Source: Zodiac, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands
- Length at study initiation: 2.5 ± 0.2 cm
- Weight at study initiation: 0.43 ± 0.08 g
ACCLIMATION
- Acclimation period: at least 12 d after delivery
- Acclimation conditions (same as test or not): same as test
- Type and amount of food during acclimation: pelleted fish food (Cyprico Crumble Excellent (300-500 µm), Coppens International bv, Helmond, The Netherlands)
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): mortality < 5%
FEEDING DURING TEST
- No feeding from 24 h prior to the test and during the total test period. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 180 mg CaCO3
- Test temperature:
- 21.1 - 22.8 °C
- pH:
- 7.6 - 7.8
- Dissolved oxygen:
- 6.5 - 9.5 mg O2/L
- Nominal and measured concentrations:
- nominal: control, 0.1, 10, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all-glass, 10 L, headspace: 1 L, fill volume: 9 L (control and highest loading rate); all-glass, 6.5 L, headspace: 1.5 L, fill volume: 4 L (lower loading rates)
- Aeration: no
- No. of organisms per vessel: 7 (control and highest loading rate), 3 (lower loading rates)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.32 g fish/L (control and highest loading rate), 0.33 g fish/L (lower loading rates)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Aadjusted ISO medium
- Culture medium different from test medium: no, same as test
- Intervals of water quality measurement: Daily in the control and the highest concentration, beginning at the start of the test (day 0).
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
EFFECT PARAMETERS MEASURED
- Mortality was recorded after 2¼, 24, 48, 72 and 96 h following the start of exposure. In addition, every afternoon from day 0 and every morning from day 1 to observe for any dead or severely distressed fish.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- yes
- Remarks:
- pentachlorophenol
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: Not observed
- Mortality of control: 0% - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality: 0% at 0.1 mg/L, 20% at 0.22 mg/L, 100% at 0.46 mg/L
- LC50: 0.25 mg/L (95% CI 0.20 - 0.41 mg/L)
- Other: Tested from 05 - 09 Jan 2012 - Reported statistics and error estimates:
- Not applicable since no mortality was recorded.
- Sublethal observations / clinical signs:
No mortality was recorded in any of the treatments.
Analytical measurements
The test substance is an organic compound with the element tin (Sn) in its molecular structure. An ICP-MS method was developed for the determination of the test substance based on the element Sn. Validation of the analytical assay was carried out, however, it was found that the accuracies on test samples prepared with the test substance were inadequate. The ICP-MS method developed was considered to be the best possible for the determination of the test substance:
- Oxygen and helium gas were used to remove the organic and tetrahydrofuran (THF) content of the test substance and accuracy samples.
- The analytical sequence had a normal number of samples and the instrument was cleaned prior to use.
- The substance must be dissolved in THF since it was not directly soluble in (aqueous) strong acids such as HNO3.
According to the results obtained it was concluded that it is not possible to use the ICP-MS method for quantification of test samples to support physico-chemical- and (eco)toxicologal studies. Other analytical techniques such as GC, HPLC and UV-Vis were not suitable for the determination of the test substance due to its hydrophobic molecular structure.
- Validity criteria fulfilled:
- yes
Reference
Description of key information
LL50 (96 h) > 100 mg/L (nominal, OECD 203)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
One experimental study is available investigating the effects of the substance to freshwater fish. The study was performed according to OECD 203 (GLP) with Cyprinus carpio under static conditions. Test solutions were prepared as loading rates of 1.0, 10.0 and 100 mg/L by adding appropriate amounts of the substance to test medium followed by 1 h stirring, 40 min settling and siphoning off the soluble fraction to be used for testing. All test solutions were clear and colorless throughout the test. Analytical measurements were not possible to be conducted since no suitable analytical method could be established (see also IUCLID section 5.1.2). No mortality was recorded after 96 h resulting in a LL50 (96 h) of > 100 mg/L (nominal). No behavioral abnormalities and sub-lethal effects were observed during testing.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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