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EC number: 240-970-4 | CAS number: 16919-31-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24.07.2017 - 08.08.2017
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 2006
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- 2009
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ammonium hexafluorozirconate
- EC Number:
- 240-970-4
- EC Name:
- Ammonium hexafluorozirconate
- Cas Number:
- 16919-31-6
- Molecular formula:
- F6Zr.2H4N
- IUPAC Name:
- ammonium hexafluorozirconate
- Test material form:
- solid: particulate/powder
1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- 10 mL of blank control and 0.32 mg/L were acidified with 100 µL HNO3 (65 %) and measured undiluted.
2 mL of 1.0 mg/L were acidified with 100 µL HNO3 (65 %) and filled up on 10 mL. Accordingly, the samples were diluted 1:5.
1 mL of 3.2 mg/L were acidified with 100 µL HNO3 (65 %) and filled up on 10 mL. Accordingly, the samples were diluted 1:10.
100 µL of 10 and 32 mg/L were acidified with 100 µL HNO3 (65 %) and filled up on 10 mL. Accordingly, the samples were diluted 1:100.
100 µL of 100 mg/L were acidified with 200 µL HNO3 (65 %) and filled up on 20 mL. Accordingly, the samples were diluted 1:200.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- For each treatment, 200 mL of the respective test item solution was mixed with the necessary amount of algal pre-culture (1.170 mL) to achieve a cell concentration of 3.5 *103 cells/mL. For the blank control, 350 mL nutrient medium was used instead of test item solution and mixed with the necessary amount of algal pre-culture (1.638 mL).
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The culture of Desmodesmus subspicatus was obtained in January 2016 by MBM Sciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- pH:
- Nominal Concentration in mg/L 0 h 72 h
Blank control 7.6 8.5
0.32 7.6 8.1
1.0 7.5 8.4
3.2 7.4 7.9
10 7.2 7.7
32 6.7 7.1
100 5.2 5.2 - Nominal and measured concentrations:
- 0.32 / 1.0 / 3.2 / 10 / 32 / 100 mg/L nominal concentration
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- < 0.32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17.09 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.02 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Reported statistics and error estimates:
- Calculation of results was performed with the help of validated software ((Microsoft Excel®). The estimation of the biological data was accomplished using the software ToxRat® Professional,version 3.2.1.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- All validity criteria were met.
No observations were made which might cause doubts concerning the validity of the study outcome. The result of the test can be considered valid. - Executive summary:
One valid experiment was performed.
The study was performed using 6 concentrations ranging from 0.32 to 100 mg/L nominal concentration. Incubation time (test systemDesmodesmus subspicatus)was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield[1]were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed in all concentrations.
At the start and at the end of the test and every 24 hours, the content of Zirconium as component of the test item (37.82 %) in the test solutions with and without algae was determined using ICP-analysis.
In the solution with algae, only in the three highest concentrated treatments Zirconium was measurable until the end of the test. That means the test item resp. Zirconium as component of the test item was present but not measurable in presence of the test organism.
In the solutions without algae, Zirconium was measurable in all test replicates until the end of the study. Nevertheless, the measured concentrations within the replicates were partly scattering. In the test solutions a decrease followed by an increase of the measured Zirconium concentration was observed. This might be caused by a reaction of the test item with the components of the test medium.
Nevertheless, the measured Zirconium concentration showed clear the presence of dis-solved test item in the test solution and the result of the algae test showed a clear dose-response relationship.
Because of these influences of the algae and the minerals in the medium, no calculation of the measured test item concentration based on measured Zirconium concentration was performed. The determination of the results was based on the nominal concentrations.
The 72h-EC50s of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
Yield (according to OECD Guideline 201) is defined as the biomass at the end of the exposure period minus the biomass at the start of the exposure period. Calculation see under 8.2 Growth and growth inhibition are quantified from measurements of the algal biomass as a function of time. Algal biomass is defined as the dry weight per volume, e.g. mg algae/L test solution. However, dry weight is difficult to measure and therefore surrogate parameters are used. Of these surrogates, cell counts are most often used.
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