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EC number: 442-390-9 | CAS number: 40573-09-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 Jun 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- MV 31
- IUPAC Name:
- MV 31
- Details on test material:
- - Name of test material (as cited in study report): MTDID 9580
- Substance type: pure active substance
- Physical state: liquid
- Analytical purity: 99%
- Expiration date of the lot/batch: 31 Dec 2007
- Stability under test conditions: Not completely soluble in test medium at a loading rate of 100 mg/L. Reacts slowly with oxygen, e.g. in the presence of moisture, resulting in acid fluorides, carboxylic acids and HF. Exposure to air was kept at a minimum during the preparation procedures, since the test substance was indicated to be volatile.
- Storage condition of test material: At room temperature in the dark under nitrogen
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Because of low solubility, MV 31 was added to reverse osmosis-purified water at a loading rate of 2000 mg/L, with 24 hours' stirring in closed 1-L test bottles. For the test, the loading rate brought to 1000 mg/L with activated sludge, sewage feed, and RO water.
- Controls: blanks, 3,5-Dichlorophenol as reference substance
- Chemical name of vehicle: No vehicle
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None noted
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: Sludge was collected from municipal sewage treatment plant: 'Waterschap de Maaskant', 's-Hertogenbosch, the Netherlands.The sludge was coarsely sieved, washed and diluted with ISO medium. A small amount of the sludge was weighed and dried at ca. 105°C to determine the amount of suspended solids (4.1 g/L of sludge, as used for the test). The sludge was collected one day before use. The pH was 7.5 on day of sampling and adjusted from 5.8 to 7.2 on the day of testing.
ISO medium (in reverse osmosis [RO]-purified tap water)
211.5 mg/L CaCl2∙2(H2O)
88.8 mg/L MgSO4∙7(H2O)
46.7 mg/L NaHCO3
4.2 mg/L KCl
ISO medium was used only for sludge preparation, not during the test.
- Method of cultivation: Because the sludge was used one day after collection, 50 mL of synthetic sewage feed was added to each litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not reported
- Test temperature:
- 18.2 - 20.5°C
- pH:
- 7.5 - 8.2
- Nominal and measured concentrations:
- Nominal concentration 1000 mg/L. No analytical monitoring.
- Details on test conditions:
- TEST SYSTEM
- Test vessels: all glass
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 1L, with 250 mL test solution, 16 mL synthetic sewage, and 200 mL sludge suspension plus sufficient RO water to bring total volume to 500 mL.
- Aeration: Continuous aeration with pipette
- No. of vessels per concentration (replicates): 2
- No. of vessels per reference (replicates): 4
- No. of vessels per control (replicates): 4 total (2 for test substance, 2 for reference substance). One analysed at the start of the test and one analysed at the end of the test
- Biomass loading rate: 1.64 g/L Mixed Liquor Sludge Solids
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ion-exchange cartridges
- Standard medium used: Synthetic sewage
- Detailed composition: 16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCI, 0.4 g CaCI2·2(H2O), 0.2 g MgSO4·7(H20), 2.8 g K2HPO4 dissolved in 1 L RO water, filtered and the pH adjusted to 7.0
OTHER TEST CONDITIONS
- Adjustment of pH: none
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Dissolved oxygen concentration
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Single concentration tested
- Justification for using less concentrations than requested by guideline: Limit test, MV 31 not soluble in water. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: No reduction in respiration rate was recorded at nominal 1000 mg/L
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: yes. However, report does not mention whether undissolved material was present in sample bottle after stirring.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 7.7 mg/L which is within the required range of 5 - 30 mg/L - Reported statistics and error estimates:
- No EC50 could be calculated for MV 31 because it proved to be non-toxic (No effects seen at highest nominal concentration tested, 1000 mg/L). For the reference substance the percentage of inhibition was plotted against the logarithm of the concentrations and the EC50 was determined using linear regression analysis.
Any other information on results incl. tables
Table, Oxygen concentration, oxygen consumption, percentage inhibition and pH for Negative controls (C), 3,5-dichlorophenol (R), and MV 31 (T) |
|||||
Replicate |
Concentration of substance (mg/L) |
Initial oxygen conc. (mg/L) |
Oxygen consumption rate (mg O2/L/h) |
Respiration inhibition (%) |
pH |
C1 |
0 |
7.4 |
46 |
8.0 |
|
C2 |
0 |
8.3 |
43 |
8.0 |
|
Mean C1+C2 |
44.5 |
||||
R1 |
1.0 |
7.8 |
42 |
5.6 |
8.1 |
R2 |
3.2 |
8.6 |
30 |
33 |
8.3 |
R3 |
10 |
8.7 |
20 |
55 |
8.2 |
R4 |
32 |
9.0 |
9 |
80 |
8.2 |
C3 |
0 |
7.3 |
48 |
7.8 |
|
C4 |
0 |
7.7 |
44 |
7.7 |
|
Mean C3+C4 |
46 |
||||
T1 |
1000 |
5.7 |
52 |
-13 |
7.5 |
T2 |
1000 |
5.7 |
54 |
-16 |
7.6 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- ontrol respiration rate variability < 15%. Reference substance 3-h EC50 7.7 mg/L (within 5-30 mg/L)
- Conclusions:
- MV 31 is not inhibitory to activated sludge respiration (3-h EC50 >1000 mg/L)
- Executive summary:
The toxicity of MV 31 to activated sludge organisms was assessed in an assay performed according to OECD TG209. The test solution was made by stirring MV 31 for 24 hours in a closed vessel at a loading rate of 2000 mg/L, with subsequence dilution to 1000 mg/L for the test. No inhibition was observed upon exposure to MV 31 at the loading rate. The 3-hour EC50 to sludge is therefore >1000 mg/L.
The study was conducted in accord with GLP criteria and satisfies the guideline requirements for test method OECD209, activated sludge respiration inhibition test. It is classified as reliable without restrictions and is suitable for Risk Assessment, Classification & Labelling, and PBT Analysis.
Results Synopsis
Test Type: Static
EC50: >1000 mg/L 95% C.I.: not applicable
NOEC: 1000 mg/L Probit Slope: not applicable
Endpoint(s) Effected: Respiration inhibition
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