2-(2-heptadec-8-enyl-2-imidazolin-1-yl)ethanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Specific details on test material used for the study:

- Physical state: liquid
- Analytical purity: common grade
- Batch No.: EN 46033.52
- Storage condition of test material: room temperature

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 0.302 g Amine O were dissolved in and made up to 25 mL with DMF
- Chemical name of vehicle: DMF

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: West Aquarium, 37431 Bad Lauterberg, Germany
- Length at study initiation (mean, range): 26 mm (23-29 mm)
- Weight at study initiation (mean and range, SD): 0.16 g (0.09-0.20 g)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions: same as test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

171 mg/L (as CaCO3)

Test temperature:

22 ± 1 °C

pH:

7.8 - 8.4

Dissolved oxygen:

84 - 100%

Nominal and measured concentrations:

- Nominal test concentrations: control, 0.1, 0.18, 0.32, 0.58, 1.0 mg/L
- Measured concentrations: not verified.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, nominal volume, fill volume: glass aquaria, 36/22/25 cm, 20 L, 15 L
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.11 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Intervals of water quality measurement: oxygen, pH, temperature daily

OTHER TEST CONDITIONS
- Photoperiod: 16 hours daily
- Light intensity: flourescent light

EFFECT PARAMETERS MEASURED: mortality and symtoms after 24, 48, 72, 96 hours

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.3

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 0.25 - 0.37 mg/L

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

0.58 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Abnormal swimming behaviour during the test period
- Mortality of control: none
- Mortality of vehicle control: none

Description of key information

The substance is acutely very toxic to fish. The 96-h LC50 value is 0.3 mg/L in zebrafish (D. rerio).

Additional information

The short-term toxicity to freshwater fish was determined in a study according to OECD TG 203 (BASF, 1989). In this study, zebrafish (D. rerio) were exposed to nominal test concentrations of 0 (control), 0.1, 0.18, 0.32, 0.58 and 1.0 mg/L for 96 hours under static conditions. DMF was used as vehicle in the preparation of test solutions. Test concentrations were not analytically verified. Mortality and symptoms were determined at test start and after 24, 48, 72 and 96 hours. The fish showed abnormal swimming behaviour during the test period. No mortality was observed in the controls (blank and vehicle). The 96-h LC50 for the test substance was determined to be 0.3 mg/L (nominal).