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EC number: 205-745-7 | CAS number: 149-73-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-02-15 to 2002-05-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ISO/DIS 14593 (1997)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of activated sludge: Marl-Ost municipal sewage treatment plant
- Storage conditions: Under aeration in the dark
- Storage length: Six days
- Preparation of inoculum for exposure: Aeration of inoculum in mineral medium for six days.
- Concentration of sludge: Dry matter content in inoculum was 3.05 g/L. Content of colony forming units (CFU) was 370000 CFU/mL
- Initial cell/biomass concentration: 5.2 mg/L
- Bidestilled water was used for the preparation of the mineral medium - Duration of test (contact time):
- 28 d
- Initial conc.:
- 49.2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- inorg. C analysis
- Details on study design:
- TEST CONDITIONS
The test was carried out in accordance with Directive 92/69/EEC C.4-C, OECD 301 B and ISO/DIS 14593.
- Composition of medium: carried out to guideline, with bidest. water
- Test temperature: 20.6-22.7°C (mean 21.8°C)
- pH: Measured at the end of the test
Blank pH7.5
Sodium benzoate pH7.0
Trimethylorthoformate pH6.9
- Aeration of dilution water: sealed vessels
- Suspended solids concentration: Initial 5.2 mg/L
- Continuous darkness: yes
- Test vessles placed on an orbital shaker and shake with 180 rpm
TEST SYSTEM
- Culturing apparatus: ca 210 ml gas-tight glass vessels sealed with butyl rubber septa and aluminium crimp seals.
The test material was dosed to a defined volume of liquid mineral medium which was already inoculated with an activated-sludge inoculum.
- Number of culture flasks/concentration:
20 vessels without test item with inoculum
20 vessels containing reference item and inoculum
20 vessels containing test item and inoculum
- Measuring equipment: Evolved CO2 measured via TIC analysis in a Shimadzu carbon analyser
SAMPLING
- Sampling frequency: sampling after 0, 3, 7, 10, 14, 21 and 28 days
- Sampling method: Sacrificial vessels. TIC analysis of the bound CO2
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
No other controls
STATISTICAL METHODS:
Calculations performed with Excel - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- No preliminary test was performed
- Test performance:
- All validity criteria were fulfilled
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 88
- Sampling time:
- 10 d
- Details on results:
- Since the degradation of Trimethylorthoformate was > 60 % in the course of 10 days after the lag phase (10% biodegradation), Trimethylorthoformate is considered to be „readily biodegradable".
Trimethylorthoformat was completely mineralised (108%) over the 28 day test period. - Results with reference substance:
- The reference item sodium benzoate was completely mineralised (97 % degradation) over the 28 day test period. It can be concluded that the inoculum used had a sufficient biological activity.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Trimethylorthoformate is considered to be „readily biodegradable”.
- Executive summary:
The ready biodegradability of Trimethylorthoformate (CAS 149-73-5) was tested according to OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test, 1992) or EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test, 1992). Activated sludge from the Marl-Ost municipal sewage treatment plant was used in the test. The test material (Trimethylorthoformate, 99.5% purity) was dosed at 49.2 mg/L .
Since the degradation of of the test substance was > 60 % in the course of 10 days after the lag phase (10% biodegradation), Trimethylorthoformate is considered to be „readily biodegradable”.
The reference item sodium benzoate was completely mineralised (97 % degradation) over the 28 day test period. It can be concluded that the inoculum used had a sufficient biological activity.
Reference
Concentration: 49.2 mg/L => 22.3 mg C/L
2.9 mg C/vessel
Test duration (days) |
TIC (mg/L) |
TIC (mg/vessel) |
Degradation % |
Degradation % |
||
|
test item |
mean of the blank value |
test item |
mean of the blank value |
|
mean |
0 |
1.86 |
1.98 |
0.24 |
0.26 |
-0.69 |
-1 |
0 |
1.74 |
1.98 |
0.23 |
0.26 |
-1.03 |
|
3 |
9.86 |
1.98 |
1.28 |
0.26 |
35.17 |
36
|
3 |
10.04 |
1.98 |
1.31 |
0.26 |
36.21 |
|
7 |
17.07 |
2.00 |
2.22 |
0.26 |
67.59 |
68
|
7 |
17.40 |
2.00 |
2.26 |
0.26 |
68.97 |
|
10 |
22.62 |
2.02 |
2.94 |
0.26 |
92.41 |
88
|
10 |
20.64 |
2.02 |
2.88 |
0.26 |
83.45 |
|
14 |
23.76 |
2.20 |
3.09 |
0.29 |
96.55 |
93
|
14 |
22.29 |
2.20 |
2.90 |
0.29 |
90.00 |
|
21 |
24.87 |
1.96 |
3.23 |
0.25 |
102.76 |
101
|
21 |
23.82 |
1.96 |
3.10 |
0.25 |
98.28 |
|
28 |
27.33 |
2.22 |
3.55 |
0.29 |
112.41 |
108
|
28 |
25.23 |
2.22 |
3.28 |
0.29 |
103.10 |
Description of key information
Readily biodegradable, OECD 301B
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The guideline study of Ernst & Diefenbach (2002), which is considered to be reliable and accurate concludes that trimethylorthoformate is readily biodegradable. The study is considered reliable for classification and for use in risk assessments.
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