Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 208-253-0 | CAS number: 518-47-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Based on mortality of test organism Rainbow trout, Channel catfish and Bluegill, the 24, 48 and 96 hrs LC50 values was given-
Rainbow trout: LC50 values at
24 hrs - 4198 mg/l
48 hrs - 3420 mg/l
96 hrs –1372 mg/l
Channel catfish: LC50 values at
24 hrs -3828 mg/l
48 hrs -2826 mg/l
96 hrs –2267 mg/l
Bluegill: LC50 values at
24 hrs -5000 mg/l
48 hrs -4898 mg/l
96 hrs –3433 mg/l
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 372 mg/L
Additional information
Various studies from peer reviewed journals for the target chemical Fluorescein sodium (Cas no. 518-47-8) were reviewed to summarize the following information:
1.The Progressive Fish – Culturist, Vol. 31 (3), Pg. no. 139-142, 1969:
Short term toxicity study to 3 different species of fish was carried out for 96 hrs.The test organism include-
1. Rainbow trout (Salmo gairdneri)
2. Channel catfish (Ictalurus punctatus) and
3. Bluegill (Lepomis macrochirus)
The dye fluorescein sodium (9-(0-carboxyphenyl)-6-hydroxy-3-isoxanthenone) was acquired from Matheson, Coleman, and Bell in a dry powder in a grade.The dye for the toxicity study was weighed separately for each vessel.Stock solutions of the water-soluble dye was prepared for concentrations of 5 and 100 p.p.m. in the antimycin-dye mixtures. Because antimycin is relatively insoluble in water, it was first dissolved in acetone and then diluted with water.The conc. of test chemical used for the study was 5 and 100 mg/l (5 and 100 ppm), respectively.
The toxicity study was performed at temperature of 21°C. LC50 value was determined at 24, 48 and 96 hrs.Based on mortality of test organism Rainbow trout, Channel catfish and Bluegill, the 24, 48 and 96 hrs LC50 values was given-
Rainbow trout:LC50 values at
24 hrs - 4198 mg/l
48 hrs - 3420 mg/l
96 hrs –1372 mg/l
Channel catfish:LC50 values at
24 hrs -3828 mg/l
48 hrs -2826 mg/l
96 hrs –2267 mg/l
Bluegill:LC50 values at
24 hrs -5000 mg/l
48 hrs -4898 mg/l
96 hrs –3433 mg/l
2. The Journal of Toxicological Sciences, Vol. 3, Pg. no. 205-214, 1978:
Short term toxicity study toOryzias latipeswas carried out for 48 hrs and survival rate (in %) was determined. As no mortality of test animal was observed after 48 hrs, the LC0 value was found to be 3,000 mg/l. Thus, the survival rate of fish after 48 hrs was found to be 100%.
3. The Journal of Toxicological Sciences, Vol. 3, Pg. no. 205-214, 1978 and 1979:
Short term toxicity study toOryzias latipeswas carried out for 48 hrs and TLm (median tolerance limit) value was determined. The TLm value was found to be 3,000 mg/l at 48 hrs.
TLm (median tolerance limit) value consider to be median lethal concentration (LC50) thus based on mortality of test fishes, the 48 hr LC50 value was found to be 3,000 mg/l.
4. Environmental Monitoring and Assessment, Volume 38, Issue 1, pp 75-96; 1995:
Ecological assessments for both acute and chronic effects for the 3 life forms of fish was conduct.During experimentThe Median Lethal Concentration which is estimated to be lethal to 50% of fish in 96 hours exposure to the test chemical (LC50) was found to be 2200mg/l.
5. Hydrogeology Journal (2001) 9:321–325:
Ecotoxicological assessments were based on the determination of acute toxicity to fish according to DIN 38412, Part 31 (Deutsches Institut für Normung e.V. 1989). For the fish test, zebra fish were used as a modification of the guideline.
The Median Lethal Concentration which is found to be lethal to 0% of zebrafish in a 96 hours exposure to the test chemical (LC0) was found to be greater than 10mg/l
The fluorescent dyes that showed no effect upon either the genotoxicity or the ecotoxicity tests were classified by the Working Group as SAFE for use in water tracing thus Uranine is classified as safe for use in water tracing.
Thus on the basis of the above all experimental results from peer reviewed journals concluded that the target chemical Fluorescein sodium (Cas no.518-47-8) can be non- hazardous to fish.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.