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EC number: 201-166-9 | CAS number: 79-00-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2001 (April 25 to June 12)
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Results issued from reviewed document but only the summary was available.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 1,1,2-trichloroethane
- EC Number:
- 201-166-9
- EC Name:
- 1,1,2-trichloroethane
- Cas Number:
- 79-00-5
- Molecular formula:
- C2H3Cl3
- IUPAC Name:
- 1,1,2-trichloroethane
- Details on test material:
- - Name of test material : 1,1,2-trichloroethane
- Analytical purity: 97.5%
- Impurities (identity and concentrations): unknown
- Lot/batch No.: 01404MQ
- Source: Sigma-Aldrich Japan Co.
- Stability under test conditions: stabilizer 2-propanol (0.5%) was used
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: Crj: CD-1 (ICR)
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 9 weeks old
IN-LIFE DATES: From: April 25 To: june 12 2001 (48 days including preliminary study)
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- Olive oil
- Details on exposure:
- PREPARATION OF DOSING SOLUTIONS: no data
- Duration of treatment / exposure:
- 24 and 48 hours
- Frequency of treatment:
- Single administration
- Post exposure period:
- 48 days including preliminary study
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0 mg/kg bw (total dose)
- Remarks:
- control
- Dose / conc.:
- 100 mg/kg bw (total dose)
- Remarks:
- nominal
- Dose / conc.:
- 200 mg/kg bw (total dose)
- Remarks:
- nominal
- Dose / conc.:
- 400 mg/kg bw (total dose)
- Remarks:
- nominal
- No. of animals per sex per dose:
- 5 males per dose
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- cyclophosphamide
Examinations
- Tissues and cell types examined:
- bone marrow
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION:
In preliminary experiments (5 males and 5 females per dose: 100, 200, 400 and 600 mg/kg), the MTD was determined to be 600 mg/kg without clear sex differences.
DETAILS OF SLIDE PREPARATION:
Staining of bone marrow cells was performed using acridine orange fluorescence dye on slide glass - Evaluation criteria:
- no data
- Statistics:
- The incidences of micronucleated polychromatic erythrocytes in negative control group and positive control group were analyzed whether they were within the range of the background variance. The significances between the treatment and negative control groups were analyzed by Fisher's exact test, following to Bonderroni's correction. Dose dependency was analyzed by Cochran-Armitage trend test.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Remarks:
- This chemical did not induce micronuclei in bone marrow cells up to 400 mg/kg (the maximum tolerated dose), although it suppressed their proliferation at 400 mg/kg
- Toxicity:
- no effects
- Remarks:
- Toxic dose > 400 mg/kg
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- See remarks on results
Any other information on results incl. tables
- The preliminary study had revealed that 1,1,2-trichloroethane induced hypoactivity in mice at doses of 400 mg/kg and above.
- Statistical results: No significant changes between negative control group and any chemical treatment group.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
No increase of micronucleated polychromatic erythrocyte counts in 2000 polychromatic erythrocytes in bone marrow cells were observed in any dose groups at 24 and 48 hours after administration. Based on these results, 1,1,2-trichloroethane was considered not genotoxic in vivo. - Executive summary:
To elucidate the genotoxic potential of 1,1,2-trichloroethane in vivo, a mammalian erythrocyte micronucleus test according to OECD test guideline 474 was conducted. 1,1,2-trichloroethane was administered by gavage in olive oil to male CD-1 mice at 100, 200 and 400 mg/kg bw, based on severe toxicity at 600 mg/kg and no sex differences on the toxicity in a previous range-finding study. The number of micronucleated PCEs in 2000 PCEs of bone marrow cells was counted at 24 and 48 h after the administration.
The 1,1, 2-trichloroethane exposed male mice showed low locomotor activity at 400 mg/kg.
Cyclophosphamide, a positive control, induced significant increase of micronucleated PCEs. No increase in the number of micronucleated PCEs in bone marrow cells were observed both 24 and 48 h after administration in any treated groups.
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