Potassium hydrogen -5-amino-4-hydroxybenzene-1,3-disulphonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

similar to OECD 203

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Potassium hydrogen -5-amino-4-hydroxybenzene-1,3-disulphonate

EC Number:

280-719-6

EC Name:

Potassium hydrogen -5-amino-4-hydroxybenzene-1,3-disulphonate

Cas Number:

83763-34-2

Molecular formula:

C6H7NO7S2.K

IUPAC Name:

potassium hydrogen -5-amino-4-hydroxybenzene-1,3-disulphonate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION OF TEST SOLUTION
Preparation of the test solution: direct dispersion in water

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: Parkwood Trout Farm, Kent, UK
- Length at study initiation:4.0 ± 0.5 cm
- Weight at study initiation: 0.96 ± 0.37 g
- Method of breeding: Holding was performed at the testing facility at 14 ± 1°C. The water was changed at least once per week. Trouts with at least 7 days of acclimatisation and mortality < 1 % within these days before the study started were used in the test.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Observations were carried out during exposure after 24, 48, 72 and 96 hours.

Test conditions

Hardness:

Total hardness: appr. 50 mg CaCO3/L

Test temperature:

14 ± 1°C

Nominal and measured concentrations:

In a range finding study nominal concentrations 1.0, 10 and 100 mg/L with two animals respectively were tested. No mortalities were observed and a limit test with the highest concentration - 100 mg/l - was conducted.

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 L, aeration

- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Biomass loading rate: fish density in the tanks was 0.48 g per litre test solution.


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Details on results:

- There was no mortality in control as well as in treated fish at any observation time point.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In this study the test item did not cause mortality to rainbow trout after 96 h.

Executive summary:

The acute toxicity of the test item to fish (rainbow trout) was determined similar to OECD-Guideline for Testing of Chemicals No. 203. A semi-static limit test with the nominal concentration of 100 mg/L was conducted. Duration of the test was 96 h. Two replicates with 10 test organisms respectively were exposed to the test item solution and one replicate serves as control. No test item analysis was carried out.

The solution of the test item didn´t show lethality at all observation time points up to 96 hours. Therefore, the LC₅₀ was laid down as > 100 mg/L (based on the nominal concentration).

The test substance has not to be classified for acute aquatic toxicity according to regulation (EC) no 1272/2008.