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EC number: 249-008-8 | CAS number: 28407-37-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- data is from peer-reviewed journal
Data source
Referenceopen allclose all
- Reference Type:
- other: authoritative database
- Title:
- Acute oral toxicity study of Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (28407-37-6) in rat
- Author:
- U.S. National Library of Medicine
- Year:
- 2 017
- Bibliographic source:
- Chemidplus Database,U.S. National Library of Medicine,2017
- Reference Type:
- review article or handbook
- Title:
- Acute oral toxicity study of Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (28407-37-6) in rat
- Author:
- S. Gangolli
- Year:
- 1 999
- Bibliographic source:
- The Dictionary of Substance and their effects,2nd edition,vol.2 (part 6)
- Reference Type:
- secondary source
- Title:
- Acute Oral Toxicity Study
- Author:
- National Technical Reports Library
- Year:
- 1 972
- Bibliographic source:
- National Technical Reports Library, Fiche No. OTS 215154,1972
- Reference Type:
- secondary source
- Title:
- Acute oral toxicity study of Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (28407-37-6) in rat
- Author:
- RTECS
- Year:
- 2 018
- Bibliographic source:
- RTECS (registry of toxic effect of chemical substance database ), 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Acute Oral toxicity test was carried out to study the effects of Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (28407-37-6) on rats.
- GLP compliance:
- not specified
- Test type:
- other: no data available
- Limit test:
- no
Test material
- Reference substance name:
- Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-)
- EC Number:
- 249-008-8
- EC Name:
- Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-)
- Cas Number:
- 28407-37-6
- Molecular formula:
- C32H16Cu2N6O16S4.4Na
- IUPAC Name:
- Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-)
- Details on test material:
- - Name of test material (IUPAC name): Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI)
- Common name: Direct Blue 218
- Molecular formula: C32H20Cu2N6Na4O16S4
- Molecular weight: 1087.82 g/mol
- Smiles notation: c12c3c(c(S(=O)(=O)[O-])cc1cc(S(=O)(=O)[O-])cc2N)N=Nc1ccc(cc1O[Cu]O3)c1cc2c(N=Nc3c(cc4c(c3O[Cu]O2)c(cc(c4)S(=O)(=O)[O-])N)S(=O)(=O)[O-])cc1.[Na+].[Na+].[Na+].[Na+]
- InChl: 1S/C32H24N6O16S4.2Cu.4Na/c33-19-11-17(55(43,44)45)5-15-9-25(57(49,50)51)29(31(41)27(15)19)37-35-21-3-1-13(7-23(21)39)14-2-4-22(24(40)8-14)36-38-30-26(58(52,53)54)10-16-6-18(56(46,47)48)12-20(34)28(16)32(30)42;;;;;;/h1-12,39-42H,33-34H2,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;;;/q;2*+2;4*+1/p-8/b37-35-,38-36-;;;;;;
- Substance type: Organic
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report):Solantine Blue 10GL
- Molecular formula :C32H20Cu2N6Na4O16S4
- Molecular weight :1087.82 g/mol
- Substance type:organic
Test animals
- Species:
- rat
- Strain:
- other: Albino SASCO
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Details on test animal
TEST ANIMALS
- Weight at study initiation: 190 to 304 grams
- Fasting period before study: the animals were fasted overnight
- Housing: The animals were individually housed in metal, wire-bottomed cages elevated above the droppings.
- Diet (e.g. ad libitum): Purina Laboratory Chow was provided ad libitum
- Water (e.g. ad libitum): water was provided ad libitum
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: deionized water
- Details on oral exposure:
- Details on exposure
VEHICLE
- Amount of vehicle (if gavage): 50% w/w
MAXIMUM DOSE VOLUME APPLIED: 6330 mg/kg bw
DOSAGE PREPARATION (if unusual):test substance was soluble in deionized water - Doses:
- 2520, 3170, 3990, 5020 and 6330 mg/kg bw
- No. of animals per sex per dose:
- Total: 25 animals
- Control animals:
- not specified
- Details on study design:
- Details on study design
- Duration of observation period following administration: 14 days
- Frequency of observations :
Gross pathology: The animals were observed for gross effect at regular intervals on the day of dosing and daily thereafter for 14 days.
- Frequency of weighing: Following the observation period, all surviving animals were weighed.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology - Statistics:
- The acute oral LD50 calculated by the method of Weil, C.S.
Results and discussion
- Preliminary study:
- Preliminary test:
the actual LD50 determination, exploratory doses were administered to six rats to estimate the order of toxicity of the test material. Based on the results of the preliminary assay, groups of five rats were dosed at levels designed to blanket the toxicity range as follows: 2520, 3170, 3990, 5020 and 6330 mg/kg bw.
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 290 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 2 720 - 3 980
- Remarks on result:
- other: 50% mortality was observed
- Mortality:
- 50% mortality was observed at dose 3290 mg/kg bw in treated animals
- Clinical signs:
- other: the following clinical signs of toxicity were observed Prostration, labored breathing, proneness, malaise, blue-black diarrhea, ptosis, morbidity, lethargy and weakness. These appeared between 45 minutes and 6 hours after dosing .Surviving animals were n
- Gross pathology:
- Gross necropsy of animals which succumbed showed generalized congestion of the lung,liver, kidneys and adrenal glands.
In all animals, tissues from all major organ systems and muscles were dyed blue to green by the compound. The color level was dose dependent. This dying effect was observed in muscle- and tendon.
Gross necropsy of animals sacrificed at termination showed no tissue damage from the administration of the compound except at the 5020 mg/kg body weight dosage. The two animals surviving at this level showed some gross indication of liver damage. - Other findings:
- no data
Any other information on results incl. tables
Table 1: Time of Death Following Dosage
Dosage Level gm/kg |
(Days) |
% mortality |
|||||||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
||
2.52 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0 |
3.17 |
0/5 |
1/5 |
1/5 |
1/5 |
1/5 |
1/5 |
1/5 |
1/5 |
1/5 |
1/5 |
2/5 |
2/5 |
2/5 |
2/5 |
40 |
3.99 |
0/5 |
2/5 |
4/5 |
4/5 |
4/5 |
4/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
100 |
5.02 |
0/5 |
2/5 |
3/5 |
3/5 |
3/5 |
3/5 |
3/5 |
3/5 |
3/5 |
3/5 |
3/5 |
3/5 |
3/5 |
3/5 |
60 |
6.33 |
0/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
100 |
*Specific gravity = 1.299 at 23° Centigrade
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified
- Conclusions:
- The lethal concentration (LD50) value for acute oral toxicity test was considered to be 3290 mg/kg bw,when male and female albino SASCO rats were treated with Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (28407-37-6) orally via gavage.
- Executive summary:
Acute oral toxicity study was done in Male and female albino SASCO rats (SASCO strain) weighing 190 to 304 grams .Each animal was examined and only healthy animals were used on this study. The animals were individually housed in metal, wire-bottomed cages elevated above the droppings.Prior to dosing, the animals were fasted overnight.food and water was provided ad libitum.Prior to the actual LD50 determination, exploratory doses were administered to six rats to estimate the order of toxicity of the test material. Based on the results of the preliminary assay, groups of five rats were dosed at levels designed to blanket the toxicity range as follows: 2520, 3170, 3990, 5020 and 6330 mg/kg bw.The test material was prepared as a 50% weight/weight dilution indeionized water. The animals were observed for gross effect at regular intervals on the day of dosing and daily thereafter for 14 days. Animals which succumbed were necropsied. Following the observation period, all surviving animals were weighed, sacrificed and necropsied. The following clinical signs of toxicity were observed Prostration, labored breathing, proneness, malaise, blue-black diarrhea, ptosis, morbidity, lethargy and weakness. These appeared between 45 minutes and 6 hours after dosing .Surviving animals were normal within 6 days after dosing. Final bodyweight records of survivors at termination showed weight gains within expected limits except in one animal at the 5020 mg/kg body weight level which showed no gain. Gross necropsy of animals which succumbed showed generalized congestion of the lung,liver, kidneys and adrenal glands. In all animals, tissues from all major organ systems and muscles were dyed blue to green by the compound. The color level was dose dependent. This dying effect was observed in muscle- and tendon.Gross necropsy of animals sacrificed at termination showed no tissue damage from the administration of the compound except at the 5020 mg/kg body weight dosage. The two animals surviving at this level showed some gross indication of liver damage.Hence, Thelethal concentration (LD50) valuefor acute oral toxicity testwas considered to be3290mg/kg bw,when male and female albino SASCO rats were treated with Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (28407-37-6) orally via gavage.
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