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EC number: 279-935-3 | CAS number: 82338-76-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro DNA damage and/or repair study
- Remarks:
- Type of genotoxicity: DNA damage and/or repair
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed publication
Data source
Reference
- Reference Type:
- publication
- Title:
- The genotoxicity of a variety of aniline derivatives in a DNA repair test with primary cultured rat hepatocytes
- Author:
- Naoki Yoshimi, Shigeyuki Sugie, Hitoshi Iwata, Kenji Niwa, Hideki Mori, Chise Hashida and Hidesuke Shimizu
- Year:
- 1 988
- Bibliographic source:
- Mutation Research, 206 (1988) 183-191
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- Hepatocyte/DNA repair test was performed to determine the mutagenic nature of N,N-Diethylaniline
- GLP compliance:
- not specified
- Type of assay:
- DNA damage and repair assay, unscheduled DNA synthesis in mammalian cells in vitro
Test material
- Reference substance name:
- N,N-diethylaniline
- EC Number:
- 202-088-8
- EC Name:
- N,N-diethylaniline
- Cas Number:
- 91-66-7
- Molecular formula:
- C10H15N
- IUPAC Name:
- N,N-diethylaniline
- Details on test material:
- - Name of test material (as cited in study report): N,N-Diethylaniline
- Molecular formula (if other than submission substance): C10H15N
- Molecular weight (if other than submission substance): 149.236 g/mol
- Substance type: organic
- Physical state: solid
- Purity: > 99%
- Impurities (identity and concentrations): no data available
Constituent 1
- Specific details on test material used for the study:
- - Name of test material: N,N-Diethylaniline
- Molecular formula: C10H15N
- Molecular weight: 149.236 g/mol
- Substance type: organic
- Physical state: solid
- Purity: > 99%
- Impurities: < 1%
Method
- Target gene:
- No data available
Species / strain
- Species / strain / cell type:
- hepatocytes: ACI Rat hepatocytes
- Details on mammalian cell type (if applicable):
- No data available
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- No data
- Metabolic activation:
- not specified
- Metabolic activation system:
- No data available
- Test concentrations with justification for top dose:
- 10-3, 10-4, 10-5, 10-6 M
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: N-2-fluorenylacetamide
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Preincubation period: No data available
- Exposure duration: 20 hrs
- Expression time (cells in growth medium):
- Selection time (if incubation with a selection agent):
- Fixation time (start of exposure up to fixation or harvest of cells): No data available
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: No data available
NUMBER OF CELLS EVALUATED: No data available
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available
OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available
OTHER: The test was performed in accordance with the method of Williams et al. Hepatocytes were isolated from the livers of male ACI rats weighing 200-250 g. The isolated hepatocytes were allowed to attach for 2 h on plastic coverslips in primary culture using Williams' Medium E. The cultures were then washed and exposed to the test chemical and [Me- 3H]thymidine (10 µCi/ml; 49 Ci/mmole) for 20 h. It was logarithmically diluted before addition to the cultures. At the end of incubation, the cultures were washed, and the coverslips were mounted on glass slides. The slides were dipped in Sakura NR-M2 photographic emulsion and exposed for 14 days. Autoradiographic grains were counted on a television screen (Olympus, type S) with a microscopic attachment.
The data were expressed as the average net counts/nucleus for 3 coverslips + the standard deviation (50 cells/coverslip). Two experiments were performed for the test compound for the assays with rat hepatocytes. - Rationale for test conditions:
- No data
- Evaluation criteria:
- The test chemical was considered positive when the mean net nuclear grain count was more than 5 grains above background and statistically greater than that of controls (unpaired t-test; P < 0.01).
- Statistics:
- Mean ± standard deviation
Results and discussion
Test results
- Species / strain:
- hepatocytes: ACI Rat hepatocytes
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- No data available
- Remarks on result:
- other: No mutagenic effect were observed
Applicant's summary and conclusion
- Conclusions:
- N,N-Diethylaniline(91-66-7) failed to induce mutation in ACI rat hepatocytes and hence is negative in the rat hepatocyte/ DNA repair test.
- Executive summary:
The hepatocyte/DNA repair test which measures unscheduled DNA synthesis (UDS) is known to be sensitive to various classes of DNA-reactive carcinogens and is regarded as a reliable short-term test for the detection of chemical carcinogens. In this study, the genotoxicity of N,N-Diethylaniline was examined by a DNA repair test with rat hepatocytes.
The test was performed basically in accordance with the method of Williams et al. The test material was dissolved in DMSO and used at dose level of 10-3, 10-4, 10-5, 10-6 M and the positive control used was N-2-fluorenylacetamide.
N,N-Diethylaniline failed to induce mutation in ACI rat hepatocytes and hence is negative in the rat hepatocyte/ DNA repair test.
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