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EC number: 206-022-9 | CAS number: 288-88-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From Jul. 5, 1985 to Nov. 7, 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study (OECD 474). no GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- no
- GLP compliance:
- no
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 1,2,4-triazole
- EC Number:
- 206-022-9
- EC Name:
- 1,2,4-triazole
- Cas Number:
- 288-88-0
- Molecular formula:
- C2H3N3
- IUPAC Name:
- 1H-1,2,4-triazole
- Details on test material:
- - Source : no data
- Lot/batch No.: 40
- Storage condition of test material: 4 ºC in the darkness.
- Physical state: Orange/yellow flakes
- Analytical purity: 97%
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River UK Limited, Margate, Kent, UK.
- Age : no data
- Weight at study initiation: 22 to 24 g.
- Housing: Animals kept in a plastic disposable cage.
- Diet (e.g. ad libitum): Scientific Feeds (Labsure) LAD 1 diet ad libitum
- Water (e.g. ad libitum): Ad libitum
- Acclimation period: 4 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ºC
- Humidity : no data
- Air changes (per hr): 30
- Photoperiod (hrs dark / hrs light): 12 hours/12 hours
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- Aqueous 1% methyl cellulose
- Details on exposure:
- All animals in all groupes were dosed with the standard volume of 20 ml/kg bw.
- Duration of treatment / exposure:
- Single dose administration
- Frequency of treatment:
- Single dose administration
- Post exposure period:
- Animals were sacrificed 24, 48, and 72 hours after administration.
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1200 mg/kg bw
Basis:
actual ingested
- No. of animals per sex per dose:
- 15 animals/sexe in control (vehicle) and test article group.
5 animals/sexe in positive control group. - Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Mitomycin C
- Route of administration: intraperitoneal injection
- Doses / concentrations: 4 mg/kg bw (0.2 mg/mL)
Examinations
- Tissues and cell types examined:
- Bone marrow cells.
- Details of tissue and slide preparation:
- The animals were killed and both femurs were dissected out from each animal. The femurs were cleared of tissue and one epiphysis removed from each bone. A direct bone marrow smear was made onto a slide (one smear/femur) and the prepared smears were air-dried, fixed in methanol, and stained.
- Evaluation criteria:
- The stained smears were examined by light microscopy to determine the incidence of micronucleated cells/1000 polychromatic erythrocytes/animal. The ratio of polychromatic to normochromatic erythrocytes for each animal was assessed by examination of at least 1000 erythrocytes.
- Statistics:
- Wilcoxon test
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- A preliminary toxicity test had been carried out to determine the toxicity of 1,2,4-triazole. From the results it was estimated that a dosage of 1200 mg/kg would be expected to kill approximately 10% of the animals within 72 hours of dosing.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
No evidence of mutagenic potential or bone marrow cell toxicity was noted in this study. - Executive summary:
In this assessment of the effect of 1,2,4 -triazole on the incidence of micronucleated polychromatic erythrocytes in mice, a dosage of 1200 mg/kg bw was administered by oral gavage. A negative and a positive controls were used. Bone marrow smears were obtained from the negative control and test compound groups at 3 sampling times; these being 24, 48 or 72 hours after dosing.
At all sampling times, mice treated with 1,2,4 -triazole showed no significant increase in the frequency of micronucleated polychromatic erythrocytes. There was no significant decrease in the ratio of polychromatic to normochromatic erythrocytes after treatment of the animals with 1,2,4 -triazole. The positive control produced large highly significant increases in the frequency of micronucleated polychromatic erythrocytes together with decreases in the ratio of polychromatic to normochromatic erythrocytes.
It is concluded from the results obtained that 1,2,4 -triazole shows no evidence of mutagenic potential or bone marrow cell toxicity when administered orally in this in vivo test procedure.
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