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EC number: 203-213-9 | CAS number: 104-55-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: cytogenicity / chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Gene mutation in vivo toxicity study of the test chemical
- Author:
- Sasaki et al
- Year:
- 1 990
- Bibliographic source:
- Mutation research
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- Mutagenic effect of the test compound was evaluated in a mouse micronucleus test.
- GLP compliance:
- not specified
- Type of assay:
- other: Mouse bone marrow micronucleus test
Test material
- Reference substance name:
- Cinnamaldehyde
- EC Number:
- 203-213-9
- EC Name:
- Cinnamaldehyde
- Cas Number:
- 104-55-2
- Molecular formula:
- C9H8O
- IUPAC Name:
- 3-phenylacrylaldehyde
- Details on test material:
- - Name of test material (as cited in study report): Cinnamaldehyde
- Molecular weight: 132.1612 g/mol
- Molecular formula: C9H8O
- Substance type: Organic
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: ddY mouse
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Details on test animals and env conditions
TEST ANIMALS
- Source: Japan SLC lnc. (Hamamatsu, Japan)
- Age at study initiation: 8 weeks (when purchased)
- Weight at study initiation: No data available
- Assigned to test groups randomly: [no/yes, under following basis: ] No data available
- Fasting period before study: No data available
- Housing: No data available
- Diet (e.g. ad libitum): No data available
- Water (e.g. ad libitum): No data available
- Acclimation period: No data available
ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data available
- Humidity (%):No data available
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): No data available
IN-LIFE DATES: From: To: No data available
Administration / exposure
- Route of administration:
- oral: unspecified
- Vehicle:
- - Vehicle(s)/solvent(s) used: Olive oil (Kosakai Seiyaku Co., Tokyo, Japan).
- Justification for choice of solvent/vehicle: The test chemical was soluble in oliv oil
- Concentration of test material in vehicle: 0, 250, 313 or 500 mg/kg with an administration volume of 10 ml/kg.
- Amount of vehicle (if gavage or dermal): No data available
- Type and concentration of dispersant aid (if powder): No data available
- Lot/batch no. (if required): No data available
- Purity: No data available - Details on exposure:
- For oral route
PREPARATION OF DOSING SOLUTIONS: The test chemical was dissolved in olive oil at a concentration of 0, 250, 313 or 500 mg/kg
DIET PREPARATION
- Rate of preparation of diet (frequency): No data available
- Mixing appropriate amounts with (Type of food): No data available
- Storage temperature of food: No data available - Duration of treatment / exposure:
- 24 hrs
- Frequency of treatment:
- No data available
- Post exposure period:
- 0, 3, 6, 9, 12, 15, 18 hours
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 250, 313 or 500 mg/kg
Basis:
no data
- No. of animals per sex per dose:
- No data available
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Positive controls
No data available
- Justification for choice of positive control(s): No data available
- Route of administration: No data available
- Doses / concentrations: No data available
Examinations
- Tissues and cell types examined:
- Bone marrow cells
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: No data available
TREATMENT AND SAMPLING TIMES ( in addition to information in specific fields): Bone marrow cells were sampled 24 h after irradiation with X-rays. The cells were at a time interval of 0, 3, 6, 9, 12, 15, 18 hours.
DETAILS OF SLIDE PREPARATION: No data available
METHOD OF ANALYSIS: No data available
OTHER: Mice were irradiated with X-rays at 200 rad using a Hitachi X-ray machine with an adjusted Victreen dosimeter. After irradiation the flavoring was administered orally at 0, 250, 313 or 500 mg/kg. Bone marrow cells were sampled 24 h after the irradiation. In the time-course study, cinnamaldehyde at 500 mg/kg was given to mice immediately after the irradiation and bone marrow cells were sampled periodically. The micronucleus assay was performed according to the methods described by Schmid (1976). - Evaluation criteria:
- The frequencies of polychromatic erythrocytes with micronuclei (MNPCEs) per 1000 polychromatic erythrocytes (PCEs), and PCEs per 1000 red blood cells per mouse were determined.
- Statistics:
- Student's t-test
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- valid
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- RESULTS OF RANGE-FINDING STUDY
- Dose range: No data available
- Solubility: No data available
- Clinical signs of toxicity in test animals: No data available
- Evidence of cytotoxicity in tissue analyzed: No data available
- Rationale for exposure: No data available
- Harvest times: No data available
- High dose with and without activation: No data available
- Other: No data available
RESULTS OF DEFINITIVE STUDY
- Types of structural aberrations for significant dose levels (for Cytogenetic or SCE assay): No data available
- Induction of micronuclei (for Micronucleus assay): The frequencies of polychromatic erythrocytes with micronuclei (MNPCEs) per 1000 polychromatic erythrocytes (PCEs), and PCEs per 1000 red blood cells per mouse were determined
- Ratio of PCE/NCE (for Micronucleus assay): No data available
- Appropriateness of dose levels and route: No data available
- Statistical evaluation: Student's t-test
Applicant's summary and conclusion
- Conclusions:
- The test chemical did not induce gene mutation in vivo in male ddY mice as observed by the mouse micronucleus test.
- Executive summary:
A mouse micronucleus test was conducted to evaluate the mutagenic nature of the test chemical in vivo in male ddYmice. The test compound was dissolved in olive oil and given to male mice at a dose range of 0, 250, 313 or 500 mg/kg. Mice were irradiated with X-rays at 200 rad using a Hitachi X-ray machine with an adjusted Victreen dosimeter. After irradiation the flavoring was administered orally at the above-mentioned dosage. Bone marrow cells were sampled 24 h after the irradiation. In the time-course study, the test chemical at 500 mg/kg was given to mice immediately after the irradiation and bone marrow cells were sampled periodically at 0, 3, 6, 9, 12, 15, 18 hours. X-ray-induced chromosome aberrations were suppressed when the test chemical was given orally to mice after X-ray irradiation. Chromosome aberrations were monitored by the occurrence of polychromatic erythrocytes with micronuclei in bone marrow cells. The frequency of micronuclei was depressed about 55-60% without toxicity of the test compounds to the bone marrow. Based on the results of the current study, it is proven that the test chemical did not induce gene mutation in vivo in male ddY mice as observed by the mouse micronucleus test.
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