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Diss Factsheets
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EC number: 925-653-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Not available
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- yes
- Remarks:
- test was run for 31 days
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Somerset-Raritan Valley Sewage Authority, Bridgewater, New Jersey
- Storage length: sludge was obtained one day before the test
- Preparation of inoculum for exposure: sludge was homogenized by mixing in a blender at medium speed for 2 min. It was then allowed to settle for 30 min. The supernatent was decanted, and the filtered supernatent used to determine microbial activity
- Pretreatment: 24 L of distilled water was placed in a 50 L carboy. The following solutions were added: 24 mL of magnesium sulfate, 24 mL of calcium chloride, 240 mL phosphate buffer, 24 mL ferric chloride, and 240 mL of sludge supernatent. The carboy was then aerated for 24 h with CO free air.
- Initial cell/biomass concentration: 10^6 colony forming units/mL
- Water filtered: yes
- Type and size of filter used, if any: glass - Duration of test (contact time):
- 31 d
- Initial conc.:
- 32.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 38.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 36.1 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 24 mL of magnesium sulfate, 24 mL of calcium chloride, 240 mL phosphate buffer, 24 mL ferric chloride, and 240 mL of sludge supernatent in distilled water
- Test temperature: 22±1 °C
- Aeration of dilution water: aerated for 24 h prior to test
TEST SYSTEM
- Number of culture flasks/concentration: 3
- Measuring equipment: electrolytic respirometer from Co-ordinated Environmental Service, Kent, England
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: manometric cell/CO2 trap
SAMPLING
- Sampling frequency: hourly
- Sampling method: aerobic respirometer
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
STATISTICAL METHODS: Results were analysed using Lotus v2.2 - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 5.0 mL of a 6,115 mg C/L solution
- Parameter:
- % degradation (O2 consumption)
- Value:
- 13.8
- Sampling time:
- 4 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 60
- Sampling time:
- 11 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 74.7
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 75.9
- Sampling time:
- 31 d
- Results with reference substance:
- The reference substance was degraded > 60%, therefore the test is valid.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance biodegraded to an extent of 74.7% after 28 days with an unacclimated sewage sludge inoculum. The test substance was determined to be readily biodegradable.
- Executive summary:
The purpose of this study was to determine the ready biodegradability of Hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, 2-25% aromatics. 35 mg/L of test substance was added to test vessels inoculated with activated sludge. The oxygen consumption of the sludge was then monitored for 31 days. Sodium benzoate was used as a reference substance. The reference substance was 88.7% degraded in 28 days, therefore the test is valid. The test substance was 74.7% degraded by day 28 and met the 10 -day window requirement for >60% degradation. The test substance is readily biodegradable.
Reference
Table: Daily Percent Biodegradation
Day |
Average % Degradation of Sodium Benzoate |
Average % Degradation of Test Material |
1 |
21.5 |
0 |
2 |
55.4 |
1.52 |
3 |
64.3 |
7.81 |
4 |
72.7 |
13.8 |
5 |
77.5 |
21.9 |
6 |
80.4 |
28.8 |
7 |
83.3 |
34.2 |
8 |
87.4 |
39.8 |
9 |
94.1 |
45.8 |
10 |
99.0 |
52.9 |
11 |
101.2 |
60.0 |
12 |
102.0 |
64.3 |
13 |
101.4 |
67.1 |
14 |
99.0 |
68.0 |
15 |
96.0 |
67.7 |
16 |
92.9 |
67.2 |
17 |
91.5 |
67.6 |
18 |
90.7 |
68.1 |
19 |
90.2 |
68.8 |
20 |
89.8 |
69.4 |
21 |
89.7 |
70.2 |
22 |
89.5 |
70.9 |
23 |
89.4 |
71.6 |
24 |
89.2 |
72.3 |
25 |
89.1 |
73.0 |
26 |
88.9 |
73.6 |
27 |
88.8 |
74.1 |
28 |
88.7 |
74.7 |
29 |
88.6 |
75.2 |
30 |
88.5 |
75.6 |
31 |
88.5 |
75.9 |
Description of key information
There is no data available for Hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, aromatics (2-25%). However, data is available for structural analogue Hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, aromatics (2-25%). This data is read across to based on analogue read across and a discussion and report on the read across strategy is provided as an attachment in IUCLID Section 13.
Hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, aromatics (2-25%), biodegraded 75% after 28 days under the conditions of the study and is readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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