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EC number: 242-355-6 | CAS number: 18472-87-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental result using OECD guidelines.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Principles of method if other than guideline:
- Biodegradation study was conducted according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test) for evaluating the percentage biodegradability of test substance 3,4,5,6-tetrachloro-2-(1,4,5,8-tetrabromo-6-hydroxy-3-oxoxanthen-9-yl)benzoic acid.
- GLP compliance:
- no
- Specific details on test material used for the study:
- CAS NO: 18472-87-2
-Chemical name: 3,4,5,6-tetrachloro-2-(1,4,5,8-tetrabromo-6-hydroxy-3-oxoxanthen-9-yl)benzoic acid
-Nature of chemical: Organic
- Purity: 96.21% (Pure dye content, by spectrophotometer)
-Stability: Stable in recommended storage conditions
-Storage conditions: 1. Ambient Temp (23 to 27 °C). Store in a cool place. Keep containers tightly closed in a dry & well - ventilated place.
2. In Refrigerator (4 °C)
3. In Freezer (-10 To 0 °C)
- Physical appearance: Red colored powder
+ Batch no: FG/15-16/0790 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: mixture of domestic waste water, surface soil and soil samples
- Details on inoculum:
- Mixed Inoculum Preparation (In-House): Domestic waste water, surface soil and soil samples from polluted sites receiving predominantly domestic waste was collected and were mixed to get diluted suspension. The inoculum was kept aerobic until being used for experiment by supplying organic and inorganic sources required by micro flora to sustain at controlled laboratory conditions. This gave the bacterial count as107to 108CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 32 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: OECD mineral medium was used for the study
- Test temperature: 20°C
- Continuous darkness: Yes
- Other: The water used in this study is deionized water.
TEST SYSTEM
- Culturing apparatus: The apparatus used in this study is BOD bottles, with glass stoppers (125 ml), BOD incubator & oxygen electrode and meter.
CONTROL AND BLANK SYSTEM
- Inoculum blank: it contains only test inoculum
- Procedure control: contains reference compound and inoculum
- Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- other: BOD and ThOD
- Value:
- 78.07
- Sampling time:
- 28 d
- Remarks on result:
- other: Other details not known
- Details on results:
- The BOD28 value of 3,4,5,6-tetrachloro-2-(1,4,5,8-tetrabromo-6-hydroxy-3-oxoxanthen-9-yl)benzoic acid (CAS No.18472-87-2) was observed to be 0.5 mgO2/mg. ThoD was determined by calculation as 0.675 mgO2/mg. % Degradation was calculated using the values of BOD and ThOD for test item and was found to be 78.07% by 32mg/l. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was found to be 84.04 %. Degradation of Sodium benzoate exceeds 78.07% after 7 days and 81.08% after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item 3,4,5,6-tetrachloro-2-(1,4,5,8-tetrabromo-6-hydroxy-3-oxoxanthen-9-yl)benzoic acid (CAS No.18472-87-2) undergoes 74.07% biodegradation after 28 days in the test condition. Thus, the test item can be inferred/considered as readily biodegradable.
- Executive summary:
28-days Closed Bottle test following the OECD guideline 301D to determine the ready biodegradability of the test item 3,4,5,6 -tetrachloro-2 -(1,4,5,8 -tetrabromo-6 -hydroxy-3 -oxoxanthen-9 -yl)benzoic acid (CAS No.18472 -87 -2). The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for the study was 32mg/l and 32mg/l, while that of inoculum was 32ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28value of3,4,5,6 -tetrachloro-2 -(1,4,5,8 -tetrabromo-6 -hydroxy-3 -oxoxanthen-9 -yl)benzoic acid (CAS No.18472 -87 -2) was observed to be 0.5mgO2/mg. ThOD was calculated as 0.675mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was found to be 74.07%. Based on the results, the test item, under the test conditions, was found to be readily biodegradable at 20 ± 1°C over a period of 28 days.
Reference
Observations and calculations:
Mean dissolved oxygen (oxygen consumed mg O2/l) from were recorded inTable 1from dissolve oxygen meter. The amount of oxygen taken up by the microbial population during biodegradation of the test item was corrected for uptake by the inoculum blank (Control) and BOD was calculated accordingly and represented inTable 2. The percent degradation of 0th, 7th, 14th, 21stand 28thwere calculated and represented inTable 3.
ThOD, BOD28and % Degradation of both reference and the test item, calculated using the formula given in OECD guideline 301D Closed Bottle Test,are summarized inTable 4. The detailed calculations of the same are given inAnnex 4 and Annex 5.
Table 5 depicts pH values of 0th, 7th, 14th, 21stand 28th day of all test vessels.
TABLE 1
DISSOLVED OXYGEN VALUES(oxygen consumed mg O2/l)
No. of Days |
Inoculum Blank (Control) |
Test Suspension
|
Procedure Control (Reference Item) |
0thDay |
8.6 |
8.4 |
8.5 |
7thDay |
6.4 |
6 |
1.1 |
14thDay |
6.5 |
4.7 |
1 |
21stday |
6.4 |
4.4 |
0.8 |
28thDay |
6.3 |
5.1 |
0.6 |
TABLE 2
BOD values (mgO2/mg)
No. of Days |
Test Suspension
|
Procedure Control (Reference Item) |
0thDay |
0 |
0 |
7thDay |
0.05 |
1.3 |
14thDay |
0.4 |
1.35 |
21stday |
0.45 |
1.375 |
28thDay |
0.5 |
1.4 |
TABLE 3
PERCENT BIODEGRDATION RESULTS
No. of Days |
Test Suspension
|
Procedure Control (Reference Item) |
0thDay |
0 |
0 |
7thDay |
7.40% |
78.07% |
14thDay |
59.25% |
81.08% |
21stday |
66.66% |
82.58% |
28thDay |
74.07% |
84.04% |
Table 4
BOD28, THOD AND % BIODEGRADATION VALUES
Method details |
BOD28(mgO2/mg) |
ThOD (mgO2/mg) |
% Biodegradation |
Test Item |
0.5 |
0.675 |
74.07% |
Reference Item |
1.4 |
1.665 |
84.04% |
TABLE 5
pH OF CLOSED BOTTLE TEST
No. of Days |
Inoculum Blank (Control) |
Test Suspension
|
Procedure Control (Reference Item) |
0thDay |
7.04 |
7.48 |
7.21 |
7thDay |
6.83 |
6.9 |
6.57 |
14thDay |
6.34 |
5.89 |
6.66 |
21stday |
6.73 |
6.73 |
6.55 |
28thDay |
6.6 |
6.93 |
6.64 |
Description of key information
The test item 3,4,5,6-tetrachloro-2-(1,4,5,8-tetrabromo-6-hydroxy-3-oxoxanthen-9-yl)benzoic acid (CAS No.18472-87-2) undergoes 74.07% biodegradation after 28 days in the test condition. Thus, the test item can be inferred/considered as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
28-days Closed Bottle test following the OECD guideline 301D to determine the ready biodegradability of the test item 3,4,5,6 -tetrachloro-2 -(1,4,5,8 -tetrabromo-6 -hydroxy-3 -oxoxanthen-9 -yl)benzoic acid (CAS No.18472 -87 -2). The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for the study was 32mg/l and 32mg/l, while that of inoculum was 32ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28value of3,4,5,6 -tetrachloro-2 -(1,4,5,8 -tetrabromo-6 -hydroxy-3 -oxoxanthen-9 -yl)benzoic acid (CAS No.18472 -87 -2) was observed to be 0.5mgO2/mg. ThOD was calculated as 0.675mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was found to be 74.07%. Based on the results, the test item, under the test conditions, was found to be readily biodegradable at 20 ± 1°C over a period of 28 days.
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