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Diss Factsheets
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EC number: 617-084-5 | CAS number: 80474-45-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20th May 2003 - 27th November 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- Two deviations occurred but didn't affect the outcome/integrity/validity of the study: It wasn't possible to issue the draft report to the proposed schedule. The inoculum pH was measured before the final dilution, not afterwards as per the protocol.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Appearance: White/off-white crystalline solid
Batch Number: H030714
Receipt Date: 26 April 2003
Storage Conditions: Room temperature, 20±10°C
Expiry Date: Not stated on recipt - Details on test solutions:
- Activate sludge was collected from one of the sludge return lines at Burley Menston sewage treatment work (Yorkshire Water), a treatment plant with a waste-water catchment that is predominantly domestic. On arrival in the laboratory, each sample was aerated by means of a compression air supply delivered through a diffuser block.
The suspended solids concentration of the activated sludge was determined by filtering a 25mL subsample through a pre-dried and pre-weighed glass microfibre filter (Whatman GF/C). The filter and retained solids were then dried at 105°C (nominal) in a conventional oven, re-weighed and the contribution made by the sludge solids were determined by difference. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The inoculum used in this study was not deliberately acclimated or adapted to Thio Acid Propionate (CCI22363) before exposure to the test substance under test conditions. The inoculum was maintained by aeration at all times and fed overnight with synthetic sewage at a rate of 50mL/L during the interval between preparation and final use.
Range-Finder:
The suspended solids concentration determined before the range-finder test was 4.0g/L, within the acceptance range of 4g/L ± 10%, and the pH was 6.02, within the acceptance range of pH 6 to 8. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21° C
- Nominal and measured concentrations:
- All mixtures comprised 16mL synthetic sewage diluted to 300mL with reverse-osmosis water and the test substance or 3,5-DCP stock solution. Inoculation entailed addition of 200mL activated sludge.
- Details on test conditions:
- The inoculum was maintained, the test mixtures incubated and the test measurements carried out in a facility where the termperature was set at a nominal 21°C. Temperatures recorded over the duration of this study were compliant with the method requirement that temperatures be maintained in the range 20±2°C.
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 9.81 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- other: 3,5-DCP
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The applied concentrations of test substance were plotted against the resulting percentage inhibition observed. No significant inhibition was observed. Consequently, the EC50 of the test substance must be greater than the highest tested concentration, 1000mg/L. Following consideration of the data, no further assessment was made. From a plot of the inhibition against dosed concentration, the EC50 for 3,5-DCP was estimated to be 12.9mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The impact of Thio Acid Propionate (CCI22363( on the respiration of activated sludge was assessed according to Guideline 209 of the Organisation for Economic Co-operation and Development (OECD). The method is identical to that described in EU Commission Directive 87/302/EEC.
Samples of activated sludge were exposed to Thio Acid Propionate (CCI22363) at a range of concentrations between 0.1 and 1000mg/L, and their respiration rates measured after 3 hours contact time. The test substance did not cause significant inhibition of the respiration rate of activated sludge at concentrations up to and including 1000mg/L. Consequently the EC50 was not determined from the results of this stud, but must be greater than 1000mg/L, the highest dose tested.
Both the Guideline validity criteria that relate to the reference inhibitor response and conformity of the control respiration rates were met. The results of this study are therefore considered valid. - Executive summary:
The impact of Thio Acid Propionate (CCI22363( on the respiration of activated sludge was assessed according to Guideline 209 of the Organisation for Economic Co-operation and Development (OECD). The method is identical to that described in EU Commission Directive 87/302/EEC.
Samples of activated sludge were exposed to Thio Acid Propionate (CCI22363) at a range of concentrations between 0.1 and 1000mg/L, and their respiration rates measured after 3 hours contact time. The test substance did not cause significant inhibition of the respiration rate of activated sludge at concentrations up to and including 1000mg/L. Consequently the EC50 was not determined from the results of this stud, but must be greater than 1000mg/L, the highest dose tested.
Both the Guideline validity criteria that relate to the reference inhibitor response and conformity of the control respiration rates were met. The results of this study are therefore considered valid.
Reference
The applied concentrations of test substance were plotted against the resulting percentage inhibition observed. No significant inhibition was observed. Consequently, the EC50 of the test substance must be greater than the highest tested concentration, 1000mg/L. Following consideration of the data, no further assessment was made. From a plot of the inhibition against dosed concentration, the EC50 for 3,5-DCP was estimated to be 12.9mg/L.
Range-Finder Test, Respiration Rate and Inhibition Data:
Treatment | Respiration Rage (mgO2/L/h) | Mean | % Inhibition |
Control | 63.331 | 68.42 | na |
70.431 | |||
72.172 | |||
67.762 | |||
1mg Thio Acid Propionate/L | 66.43 | 69.62 | 0 |
72.82 | |||
10mg Thio Acid Propionate/L | 71.57 | 70.88 | 0 |
70.20 | |||
100mg Thio Acid Propionate/L | 66.55 | 70.54 | 0 |
74.54 | |||
5mg 3,5-DCP/L | 53.76 | na | 21 |
15mg 3,5-DCP/L | 30.00 | na | 56 |
45mg 3,5-DCP/L | 9.81 | na | 86 |
1 = start-of-series control
2 = end-of-series control
na = not applicable
Description of key information
The impact of Thio Acid Propionate (CCI22363( on the respiration of activated sludge was assessed according to Guideline 209 of the Organisation for Economic Co-operation and Development (OECD). The method is identical to that described in EU Commission Directive 87/302/EEC.
Samples of activated sludge were exposed to Thio Acid Propionate (CCI22363) at a range of concentrations between 0.1 and 1000mg/L, and their respiration rates measured after 3 hours contact time. The test substance did not cause significant inhibition of the respiration rate of activated sludge at concentrations up to and including 1000mg/L. Consequently the EC50 was not determined from the results of this stud, but must be greater than 1000mg/L, the highest dose tested.
Both the Guideline validity criteria that relate to the reference inhibitor response and conformity of the control respiration rates were met. The results of this study are therefore considered valid.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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