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Diss Factsheets
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EC number: 220-120-9 | CAS number: 2634-33-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage)
- Principles of method if other than guideline:
- Method: other: INVITTOX Protocol No 124. In-vitro Bovine Corneal Opacity and Permeability Assay (BCOP).
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,2-benzisothiazol-3(2H)-one
- EC Number:
- 220-120-9
- EC Name:
- 1,2-benzisothiazol-3(2H)-one
- Cas Number:
- 2634-33-5
- Molecular formula:
- C7H5NOS
- IUPAC Name:
- 1,2-benzisothiazol-3(2H)-one
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- Batch LHW 1355
>99%
Test animals / tissue source
- Species:
- other: In-vitro Bovine Corneal Opacity and Permeability Assay (BCOP)
Test system
- Vehicle:
- physiological saline
- Amount / concentration applied:
- other: 7500, 750 and 75 ppm
- Duration of treatment / exposure:
- 2 hour(s)
- Details on study design:
- The eye irritancy potential of 1,2-benzisothiazolin-3-one was assessed using the in-vitro BCOP assay at concentrations of 75, 750 and 7500 ppm. 1,2-benzisothiazolin- 3-one was found to be non-irritant using the in-vitro scoring method at the dilutions tested. This is comparable to the minimal score in the corresponding Dralze assay. A 7500 ppm solution of 1,2-benzisothiazolin-3-one was prepared as its sodium salt. The pH of the solution was adjusted to 8.43, being the pH nearest neutral at which this concentration of BIT was soluble. BIT was tested at 7500 ppm, 750 ppm and 75 ppm. Each dilution was tested in the experimental system on a separate day (n=9) against negative (saline) and positive (ethanol) controls. The In-vitro Scores obtained were related to the Dralze classification system.
Comment: other: the procedure was conducted 9 times at each concentration
Results and discussion
In vitro
Resultsopen allclose all
- Irritation parameter:
- in vitro irritation score
- Run / experiment:
- 7500 ppm
- Value:
- 3.012
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Irritation parameter:
- in vitro irritation score
- Run / experiment:
- 750 ppm
- Value:
- 0.666
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Irritation parameter:
- in vitro irritation score
- Run / experiment:
- 75 ppm
- Value:
- -0.207
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
BIT was found to be non-irritant using the in-vitro scoring method when tested at dilutions of 75 ppm, 750 ppm and 7500 ppm. This is comparable to the minimal score in the corresponding Draize assay.
Applicant's summary and conclusion
- Conclusions:
- BIT was found to be non-irritant using the in-vitro scoring method when tested at dilutions of 75 ppm, 750 ppm and 7500 ppm. This is comparable to the minimal score in the corresponding Draize assay.
- Executive summary:
The acute eye irritation potential of the substance was determined according to a method similar to OECD Guideline 437. The in vitro bovine corneal opacity assay was performed with the substance at concentrations of 0, 75, 750 and 7500 ppm. The in vitro irritation scores of the test concentrations were found to be 3.012, 0.666 and -0.207 respectively for 7500, 750 and 75 ppm. Under the study conditions, no prediction of eye irritation could be made based on the score of 3.012 at the highest test concentration (Smith, 2003).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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