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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 266-719-9 | CAS number: 67564-91-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- biochemical or cellular interactions
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
- Principles of method if other than guideline:
- In-vitro study on acetylcholinesterase activity inhibition with plasma from rat and dog and pure AChE using structural analogues of the parent and a metabolite. I50 determinaton for most potent inhibitor.
- GLP compliance:
- no
- Type of method:
- in vitro
- Endpoint addressed:
- neurotoxicity
Test material
Constituent 1
- Specific details on test material used for the study:
- Storage with silica gel at 4-10°C
Administration / exposure
- Vehicle:
- other: 50 mM phosphate buffer pH 7.2
- Details on study design:
- Results are a mean of ten measurements
The negative control was performed by adding water instead of the test substance.
The three test substances were each applied at a concentration of 4 mM.
Two test substances were structural analogues to a metabolite of the target substance.
One test substance was a structural analogue to the target substance.
The analogues were chosen to retain the structural groups considered relevant for AChE activity. The target substance itself could not be tested as such as it lacked sufficient solubility. There was no method to synthesize the metabolite.
Examinations
- Examinations:
- Cholinesterase activity was determined using a kit by Boehringer Mannheim, Germany which is based on the Elman method. Acetylthiocholine iodide is used as substrate and 5,5´-dithiobisnitrobenzoic acid (0.25 mmol/l) as indicator. The test is performed at 25°C.
The substrate concentrations applied were from 0.00001 to 0.01 mol/L.
Results and discussion
- Details on results:
- The analogue to the target substance did not inhibit rat and dog plasma cholinesterase activity and had a slight inhibitory effect on electrophorus electricus AChE activity.
Both metabolite analogues had a significant inhibitory effect on rat and dog (ca -65%) and on electrophorous electricus (ca - 82%) AChE activity.
The I50 concentrations were 3.5 mM for e.e. AChE and 0.425 mM for plasma AChE. The test substances are reversible competitive inhibitors of AChE.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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