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EC number: 440-520-9 | CAS number: 204583-39-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Repeated dose toxicity: oral
Administrative data
- Endpoint:
- short-term repeated dose toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 407 (Repeated Dose 28-Day Oral Toxicity Study in Rodents)
- GLP compliance:
- yes
- Limit test:
- no
Test material
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: HanBrhWIST (SPF)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS- Source: RCC Ltd, Biotechnology & Animal Breeding Division, Fuellinsdorf / Switzerland- Age at study initiation: 7 weeks- Weight at acclimatization: Males: 129.6 - 161.0 grams (mean 139.8 grams), Females: 109.7- 130.7 grams (mean 119.1 grams)- Housing: groups of five animals- Diet (e.g. ad libitum): Pelleted standard Provimi Kliba 3433 (batch nos. 119/01 and 05/02) rat maintenance diet (Provimi Kliba AG, Kaiseraugst/ Switzerland) was available ad libitum.- Water (e.g. ad libitum): Community tap-water from Itingen was available ad libitum in water bottles.- Acclimation period: one weekENVIRONMENTAL CONDITIONS- Temperature (°C): 22 ± 3- Humidity (%): 30-70- Air changes (per hr): 10-15- Photoperiod (hrs dark / hrs light): 12 hours fluorescent light/12 hours dark
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Remarks:
- PEG 300
- Details on oral exposure:
- PREPARATION OF DOSING SOLUTIONS: The test item was weighed into a glass beaker on a tared Mettler balance and the vehicle added. The mixtures were prepared using a magnetic stirrer and stored at room temperature (17-23°C). Homogeneity of the test item in the vehicle was maintained during the daily administration period using a magnetic stirrer.VEHICLE- Lot/batch no. (if required): 433337/1
- Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- Test item/vehicle mixtures were prepared and about 2 g (weighed to the third decimal place) of each mixture was weighed into a 100-mLvolumetric flask by RCC Ltd, Toxicology Division, Itingen. These samples for analysis of concentration and homogeneity were delivered to the analytical laboratory where they were stored deep-frozen (about -20 °C) until analysis. Storage stability samples were collected after storage (2 hours, 7 days) and then delivered to the analytical laboratory where they were stored deep-frozen until analysis.The delivered samples were dissolved in about 70 mL of acetonitrile/tetrahydrofuran (1-t-l v/v) using an ultrasonic bath. Then, the 100-mL volumetric flasks were filled to the mark with acetonitrile/tetrahydrofuran (1-1-1 v/v). Depending on the dose group, the latter sample solutions were further diluted with acetonitrile/tetrahydrofuran (l-i-l v/v) to yield concentrations within the calibration range. Finally, a defined aliquot was quantified by HPLC.
- Duration of treatment / exposure:
- 28 days
- Frequency of treatment:
- once daily
Doses / concentrationsopen allclose all
- Dose / conc.:
- 50 mg/kg bw/day (actual dose received)
- Dose / conc.:
- 200 mg/kg bw/day (actual dose received)
- Dose / conc.:
- 1 000 mg/kg bw/day (actual dose received)
- No. of animals per sex per dose:
- 5 animals; additional five animals per sex were available in groups 1 (controls) and 4 (high dose) for the assessment of reversibility of effects.
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- - Dose selection rationale: Based upon the results of a non-GLP 5-day doserange-finding study (RCC Study Number 842521) in which the test substance was administered by gavage to 2 rats per group and sex.
- Positive control:
- no
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: Yes - Time schedule: dailyDETAILED CLINICAL OBSERVATIONS: Yes - Time schedule: weeklyBODY WEIGHT: Yes - Time schedule for examinations: weeklyFOOD CONSUMPTION AND COMPOUND INTAKE:- Food consumption for each animal determined and mean daily diet consumption calculated as g food/kg body weight/day: YesHAEMATOLOGY: Yes- Time schedule for collection of blood: At the end of the dosing and the treatment-free recovery period- Anaesthetic used for blood collection: Yes, light isoflurane- Animals fasted: Yes, in metabolism cages for approximately 18 hours before blood sampling but allowed access to water ad libitum.- Parameters examined: Erythrocyte count, Hemoglobin, Hematocrit, Mean corpuscular volume, Mean corpuscular hemoglobin, Mean corpuscular hemoglobin concentration, Platelet count, Reticulocyte count, Reticulocyte fluorescence ratios, Nucleated erythrocytes (normoblasts), Heinz bodies, Methemoglobin, Total leukocyte count, Differential leukocyte count, Red blood cell morphology, Thromboplastin time (= prothrombin time), activated partial thromboplastin timeCLINICAL CHEMISTRY: Yes- Time schedule for collection of blood: At the end of the dosing and the treatment-free recovery period- Animals fasted: Yes, in metabolism cages for approximately 18 hours before blood sampling but allowed access to water ad libitum.- Parameters examined: Glucose, Urea, Creatinine, Uric Acid, Bilirubin total, Cholesterol total, Triglycerides, Phospholipids, Aspartate aminotransferase, Alanine aminotransferase, Lactate dehydrogenase, Creatine kinase, Alkaline phosphatase, Gamma-glutamyl transferase, Calcium, Phosphorus, Sodium, Potassium, Chloride, Albumin, Protein total, Globulin, Albumin/Globulin ratioURINALYSIS: Yes- Time schedule for collection of urine: at the end of the dosing and the treatment-free recovery period- Metabolism cages used for collection of urine: Yes - Animals fasted: Yes, for approximately 18 hours before sampling- Parameters examined: Volume (18-hour), Specific gravity, Osmolality, Color, Appearance, pH, Protein, Glucose, Ketone, Bilirubin, Blood, Nitrite, Urobilinogen, Urine sediment, Red blood cells, White blood cells, Crystals (Triple phosphate)OTHER:GRIP STRENGTH: Forelimb and hind limb grip strength measurements were performed using a push-pull strain gauge (Mecmesin, AGF 25N).LOCOMOTOR ACTIVITY: Locomotor (decreased or increased) activity was measured quantitatively with Activity Monitor AM 1052 system (Benwick Electronic Equipment Design Manufacture, England). Animals were randomized and monitored during the fourth treatment week for a 60-minute period and the total activity of this time period was recorded. Low beams count was reported in 15-minute intervals as well as the total activity of the measuring period.
- Sacrifice and pathology:
- GROSS PATHOLOGY: YesHISTOPATHOLOGY: Yes
- Statistics:
- The following statistical methods were used to analyze the grip strength, locomotor activity, body weight, organ weights and ratios, as well as clinical laboratory data:• The Dunnett-test (many to one t-test) based on a pooled variance estimate was applied if the variables could be assumed to follow a normal distribution for the comparison of the treated groups and the control groups for each sex.• The Steel-test (many-one rank test) was applied instead of the Dunnett-test when the data could not be assumed to follow a normal distribution.• Student's t-test was applied to grip strength and locomotor activity.• Fisher's exact-test was applied to the macroscopic findings.
Results and discussion
Results of examinations
- Clinical signs:
- no effects observed
- Description (incidence and severity):
- No test item-related clinical signs of toxicological relevance were noted during daily or weekly observations (weeks 1-3).
- Mortality:
- no mortality observed
- Description (incidence):
- All animals survived until scheduled necropsy.
- Body weight and weight changes:
- no effects observed
- Description (incidence and severity):
- No test item-related changes in body weight were noted at any dose level tested.
- Food consumption and compound intake (if feeding study):
- no effects observed
- Description (incidence and severity):
- No test item-related changes in food consumption were noted at any dose level tested.
- Haematological findings:
- no effects observed
- Description (incidence and severity):
- No test item-related changes of toxicological significance were noted after treatment or recovery in the hematology parameters at any dose level.
- Clinical biochemistry findings:
- no effects observed
- Description (incidence and severity):
- The clinical biochemistry parameters of the test item-treated rats were similar to those of the controls after the treatment and recovery periods.
- Urinalysis findings:
- no effects observed
- Description (incidence and severity):
- The urinalysis parameters of the test item-treated rats were unaffected after the treatment and recovery periods
- Behaviour (functional findings):
- effects observed, treatment-related
- Description (incidence and severity):
- FUNCTIONAL OBSERVATIONAL BATTERY: No test item-related clinical signs of toxicological relevance were noted during functional observations (week 4).GRIP STRENGTH: No test item-related differences in fore- or hindlimb grip strength values were noted at any dose level tested.LOCOMOTOR ACTIVITY: Slight reductions in mean locomotor activity were noted in both sexes treated with 1000 mg/kg/day. These differences were transient, but contributed to slightly lower total locomotor activity on both sexes and was considered to be a minor effect related to the test item. The locomotor activity values of the rats treated with 50 mg/kg/day or 200 mg/kg/day compared favorably with those of the controls.
- Organ weight findings including organ / body weight ratios:
- no effects observed
- Description (incidence and severity):
- No test item-related differences in the absolute or relative organ weights were noted at any dose level after four weeks' treatment or two weeks' recovery.
- Gross pathological findings:
- no effects observed
- Description (incidence and severity):
- No test item-related macroscopic changes were noted at any dose level after the treatment or recovery periods.
- Histopathological findings: non-neoplastic:
- no effects observed
- Description (incidence and severity):
- No test item-related microscopic changes were noted at any dose level after the treatment or recovery periods.
Effect levels
open allclose all
- Dose descriptor:
- NOEL
- Effect level:
- 200 mg/kg bw/day (actual dose received)
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- behaviour (functional findings)
- Key result
- Dose descriptor:
- NOAEL
- Effect level:
- 1 000 mg/kg bw/day (actual dose received)
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: No adverse effects observed at this dose level
Target system / organ toxicity
- Critical effects observed:
- no
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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